Abstract
Grafting provides a simple way to generate chimeric plants with regions of different genotypes and thus to assess the cell autonomy of gene action. The technique of grafting has been widely used in other species, but in Arabidopsis, its small size makes the process rather more demanding. However, there are now several well-established grafting procedures available, which we described here, and their use has already contributed greatly to understanding of such processes as shoot branching control, flowering, disease resistance, and systemic silencing.
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References
Booker JP, Chatfield SP, Leyser O (2003) Auxin acts in xylem-associated or medullary cells to mediate apical dominance. Plant Cell 15:495–507
Furner IJ et al (1996) Clonal analysis of the late flowering fca mutant of Arabidopsis thaliana: Cell fate and cell autonomy. Development 122:1041–1050
Jenik PD, Irish VF (2000) Regulation of cell proliferation patterns by homeotic genes during Arabidopsis floral development. Development 126:1267–1276
Woodrick R et al (2000) Arabidopsis embryonic shoot fate map. Development 127:8 13–820
Rhee SY, Somerville CR (1995) Flat-surface grafting in Arabidopsis thaliana. Plant Mol Bol Rep 13:118–123
Turnbull CGN, Booker JP, Leyser HMO (2002) Micrografting techniques for testing long-distance signalling in Arabidopsis. Plant J 32:255–262
Ayre BG, Turgeon R (2004) Graft transmission of a floral stimulant derived from CONSTANS. Plant Physiol 13:2271–2278
Sorefan K et al (2003) MAX4 and RMS1 are orthologous dioxygenase-like genes that regulate shoot branching in Arabidopsis and pea. Genes Dev 17:1469–1474
Booker J et al (2004) MAX3/CCD7 is a carotenoid cleavage dioxygenase required for the synthesis of a novel plant signaling molecule. Curr Biol 14:1232–1238
An HL et al (2004) CONSTANS acts in the phloem to regulate a systemic signal that induces photoperiodic flowering of Arabidopsis. Development 131:3615–3626
Van Norman JM, Frederick RL, Sieburth LE (2004) BYPASS1 negatively regulates a root-derived signal that controls plant architecture. Curr Biol 14:1739–1746
Ragni L et al (2011) Mobile gibberellin directly stimulates Arabidopsis hypocotyl xylem expansion. Plant Cell 23:1322–1336
Green LS, Rogers EE (2004) FRD3 controls iron localization in Arabidopsis. Plant Physiol 136:2523–2531
Widiez T et al (2011) HIGH NITROGEN INSENSITIVE 9 (HNI9)-mediated systemic repression of root NO3 − uptake is associated with changes in histone methylation. Proc Natl Acad Sci USA 108:13329–13334
Lin SI et al (2008) Regulatory network of microRNA399 and PHO2 by systemic signaling. Plant Physiol 147:732–746
Pant BD et al (2008) MicroRNA399 is a long-distance signal for the regulation of plant phosphate homeostasis. Plant J 53:731–738
Xia YJ et al (2004) An extracellular aspartic protease functions in Arabidopsis disease resistance signaling. EMBO J 23:980–988
Brosnan CA et al (2007) Nuclear gene silencing directs reception of long-distance mRNA silencing in Arabidopsis. Proc Natl Acad Sci USA 104:14741–14746
Melnyk CW et al (2011) Mobile 24 nt small RNAs direct transcriptional gene silencing in the root meristems of Arabidopsis thaliana. Curr Biol 21:1678–1683
Mugford S et al (2007) The Arabidopsis transmissible wound signal. Comp Biochem Physiol Part A Mol Integr Physiol 146:S242
Wilson AK et al (1990) A dominant mutation in Arabidopsis confers resistance to auxin, ethylene and abscisic acid. Mol Gen Genet 222:377–383
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plantarum 15:473–497
Gray WM et al (1998) High temperature promotes auxin-mediated hypocotyl elongation in Arabidopsis. Proc Natl Acad Sci USA 95:7197–7202
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Bainbridge, K., Bennett, T., Crisp, P., Leyser, O., Turnbull, C. (2014). Grafting in Arabidopsis. In: Sanchez-Serrano, J., Salinas, J. (eds) Arabidopsis Protocols. Methods in Molecular Biology, vol 1062. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-580-4_7
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DOI: https://doi.org/10.1007/978-1-62703-580-4_7
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