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Methodological Approaches to Study Histamine Pharmacology in the Kidney: From Cell Culture and Cell Imaging to Functional Assays and Electron Microscopy

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Histamine Receptors as Drug Targets

Part of the book series: Methods in Pharmacology and Toxicology ((MIPT))

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Abstract

The best approach to study the complexity of renal pathophysiology is to use living organisms. However, to limit the number of animal experiments undertaken and obtain first in humans results, in vitro models can be extremely helpful options. Moreover, in vitro assays allow us to characterize a new pharmacological target, defining also the selectivity, efficacy, and potency of ligands in a specific cell type. This chapter aims to describe the steps taken to obtain a suitable model to study histamine pharmacology in renal cells, starting from the cell isolation to the morpho-functional assays. The in vitro system herein reported mostly reflects the in vivo situation as demonstrated by our previously published data.

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Authors and Affiliations

  1. Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy

    Alessandro Pini

  2. Dipartimento di Scienza e Tecnologia del Farmaco, University of Turin, Via P. Giuria 9, 10125, Turin, Italy

    Eleonora Veglia & Arianna Carolina Rosa

  3. Department of Medical Sciences, University of Turin, Turin, Italy

    Cristina Grange

Authors
  1. Alessandro Pini
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  2. Eleonora Veglia
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  3. Cristina Grange
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  4. Arianna Carolina Rosa
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Corresponding author

Correspondence to Arianna Carolina Rosa .

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Editors and Affiliations

  1. Department of Pharmacology Medical School, National and Kapodistrian University of Athens, Athens, Greece

    Ekaterini Tiligada

  2. Centre for Experimental Medicine, School of Medicine, Dentistry and Biomedical Sciences, Queen’s University Belfast, Belfast, Northern Ireland, United Kingdom

    Madeleine Ennis

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Pini, A., Veglia, E., Grange, C., Rosa, A.C. (2017). Methodological Approaches to Study Histamine Pharmacology in the Kidney: From Cell Culture and Cell Imaging to Functional Assays and Electron Microscopy. In: Tiligada, E., Ennis, M. (eds) Histamine Receptors as Drug Targets. Methods in Pharmacology and Toxicology. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6843-5_10

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  • DOI: https://doi.org/10.1007/978-1-4939-6843-5_10

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