Articles

1. Fluorine-19 labeling of the tryptophan residues in the G protein-coupled receptor NK1R using the 5-fluoroindole precursor in Pichia pastoris expression

   • Benxun Pan
   • Canyong Guo
   • Kurt Wüthrich

   Article 30 March 2024

   

2. Enabling site-specific NMR investigations of therapeutic Fab using a cell-free based isotopic labeling approach: application to anti-LAMP1 Fab

   • Arthur Giraud
   • Lionel Imbert
   • Jérôme Boisbouvier

   Article 28 March 2024

   

3. Prediction of order parameters based on protein NMR structure ensemble and machine learning

   • Qianqian Wang
   • Zhiwei Miao
   • Maili Liu

   Article 26 March 2024

   

4. DNP-assisted solid-state NMR enables detection of proteins at nanomolar concentrations in fully protonated cellular milieu

   • Whitney N. Costello
   • Yiling Xiao
   • Kendra K. Frederick

   Article 23 March 2024

   

5. Decorating phenylalanine side-chains with triple labeled ¹³C/¹⁹F/²H isotope patterns

   • Giorgia Toscano
   • Julian Holzinger
   • Roman J. Lichtenecker

   Article Open access 21 March 2024

   

6. Reconstitution and resonance assignments of yeast OST subunit Ost4 and its critical mutant Ost4V23D in liposomes by solid-state NMR

   • Bharat P. Chaudhary
   • Jochem Struppe
   • Smita Mohanty

   Article 29 February 2024

   

7. Fluorine labelling for in situ ¹⁹F NMR in oriented systems

   • Kieran T. Cockburn
   • Brian D. Sykes

   Brief Report 26 February 2024

   

8. A comprehensive assessment of selective amino acid ¹⁵N-labeling in human embryonic kidney 293 cells for NMR spectroscopy

   • Ganesh P. Subedi
   • Elijah T. Roberts
   • Adam W. Barb

   Brief Report 26 February 2024

   

9. Beyond slow two-state protein conformational exchange using CEST: applications to three-state protein interconversion on the millisecond timescale

   • Ved Prakash Tiwari
   • Debajyoti De
   • Pramodh Vallurupalli

   Article 03 January 2024 Pages: 39 - 60

   

10. The NMR signature of maltose-based glycation in full-length proteins

    • Pauline Defant
    • Christof Regl
    • Mario Schubert

    Article Open access 20 December 2023 Pages: 61 - 72

    

11. Short and long range 2D ¹⁵N–¹⁵N NMR correlations among peptide groups by novel solid state dipolar mixing schemes

    • Sungsool Wi
    • Conggang Li
    • Lucio Frydman

    Article 16 December 2023 Pages: 19 - 30

    

12. ¹⁹F-NMR studies of the impact of different detergents and nanodiscs on the A_2A adenosine receptor

    • Francisco Mendoza-Hoffmann
    • Canyong Guo
    • Kurt Wüthrich

    Article 10 December 2023 Pages: 31 - 37

    

13. Uniform [¹³C,¹⁵N]-labeled and glycosylated IgG1 Fc expressed in Saccharomyces cerevisiae

    • Alexander R. Davis
    • Elijah T. Roberts
    • Adam W. Barb

    Article 21 November 2023 Pages: 9 - 18

    

14. Efficient determination of the accessible conformation space of multi-domain complexes based on EPR PELDOR data

    • Sina Kazemi
    • Anna Lopata
    • Volker Dötsch

    Article Open access 15 November 2023 Pages: 261 - 269

    

15. 5D solid-state NMR spectroscopy for facilitated resonance assignment

    • Alexander Klein
    • Suresh K. Vasa
    • Rasmus Linser

    Article Open access 09 November 2023 Pages: 229 - 245

    

16. Paramagnetic NMR to study iron sulfur proteins: ¹³C detected experiments illuminate the vicinity of the metal center

    • Leonardo Querci
    • Deborah Grifagni
    • Mario Piccioli

    Article Open access 18 October 2023 Pages: 247 - 259

    

17. Synthesis of a ¹³C-methylene-labeled isoleucine precursor as a useful tool for studying protein side-chain interactions and dynamics

    • Theresa Höfurthner
    • Giorgia Toscano
    • Robert Konrat

    Article Open access 11 October 2023 Pages: 1 - 8

    

18. Breaking boundaries: TINTO in POKY for computer vision-based NMR walking strategies

    • Andrea Estefania Lopez Giraldo
    • Zowie Werner
    • Woonghee Lee

    Article 07 October 2023 Pages: 217 - 228

    

19. A thermosensitive gel matrix for bioreactor-assisted in-cell NMR of nucleic acids and proteins

    • Matej Dzurov
    • Šárka Pospíšilová
    • Jan Ryneš

    Article Open access 09 September 2023 Pages: 203 - 215

    

20. Strategies for acquisition of resonance assignment spectra of highly dynamic membrane proteins: a GPCR case study

    • Evan J. van Aalst
    • Jun Jang
    • Benjamin J. Wylie

    Article 26 July 2023 Pages: 191 - 202

    

21. Improved spectral resolution of [¹³C,¹H]-HSQC spectra of aromatic amino acid residues in proteins produced by cell-free synthesis from inexpensive ¹³C-labelled precursors

    • Damian Van Raad
    • Thomas Huber
    • Gottfried Otting

    Article Open access 20 June 2023 Pages: 183 - 190

    

22. E. coli “Stablelabel” S30 lysate for optimized cell-free NMR sample preparation

    • Roman Levin
    • Frank Löhr
    • Frank Bernhard

    Article Open access 13 June 2023 Pages: 131 - 147

    

23. Studying micro to millisecond protein dynamics using simple amide ¹⁵N CEST experiments supplemented with major-state R₂ and visible peak-position constraints

    • Nihar Pradeep Khandave
    • Ashok Sekhar
    • Pramodh Vallurupalli

    Article 10 June 2023 Pages: 165 - 181

    

24. Efficient 18.8 T MAS-DNP NMR reveals hidden side chains in amyloid fibrils

    • Alons Lends
    • Nicolas Birlirakis
    • Kong Ooi Tan

    Article 08 June 2023 Pages: 121 - 130

    

25. ¹H-detected characterization of carbon–carbon networks in highly flexible protonated biomolecules using MAS NMR

    • Salima Bahri
    • Adil Safeer
    • Marc Baldus

    Article Open access 08 June 2023 Pages: 111 - 119

    

26. Non-uniform sampling of similar NMR spectra and its application to studies of the interaction between alpha-synuclein and liposomes

    • Alexandra Shchukina
    • Thomas C. Schwarz
    • Krzysztof Kazimierczuk

    Article Open access 26 May 2023 Pages: 149 - 163

    

27. A methyl-TROSY based ¹³C relaxation dispersion NMR experiment for studies of chemical exchange in proteins

    • Vitali Tugarinov
    • James L. Baber
    • G. Marius Clore

    Article 25 April 2023 Pages: 83 - 91

    

28. NMR detection and conformational dependence of two, three, and four-bond isotope shifts due to deuteration of backbone amides

    • Andrei T. Alexandrescu
    • Aurelio J. Dregni
    • Carolyn M. Teschke

    Article 24 April 2023 Pages: 93 - 109

    

29. Sparse pseudocontact shift NMR data obtained from a non-canonical amino acid-linked lanthanide tag improves integral membrane protein structure prediction

    • Kaitlyn V. Ledwitch
    • Georg Künze
    • Jens Meiler

    Article 04 April 2023 Pages: 69 - 82

    

30. Assessing the applicability of ¹⁹F labeled tryptophan residues to quantify protein dynamics

    • Christina Krempl
    • Remco Sprangers

    Article Open access 14 January 2023 Pages: 55 - 67

    

31. Three segment ligation of a 104 kDa multi-domain protein by SrtA and OaAEP1

    • Stephan B. Azatian
    • Marella D. Canny
    • Michael P. Latham

    Article Open access 21 December 2022 Pages: 25 - 37

    

32. Water irradiation devoid pulses enhance the sensitivity of ¹H,¹H nuclear Overhauser effects

    • V. S. Manu
    • Cristina Olivieri
    • Gianluigi Veglia

    Article 19 December 2022 Pages: 1 - 14

    

33. Efficiently driving protein-based fragment screening and lead discovery using two-dimensional NMR

    • Chen Peng
    • Andrew T. Namanja
    • Andrew M. Petros

    Article 13 December 2022 Pages: 39 - 53

    

34. Investigation of lipid/protein interactions in trifluoroethanol-water mixtures proposes the strategy for the refolding of helical transmembrane domains

    • Vladislav V. Motov
    • Erik F. Kot
    • Konstantin S. Mineev

    Article 30 November 2022 Pages: 15 - 24

    

35. Band-selective universal 90° and 180° rotation pulses covering the aliphatic carbon chemical shift range for triple resonance experiments on 1.2 GHz spectrometers

    • Stella Slad
    • Wolfgang Bermel
    • Burkhard Luy

    Article Open access 24 November 2022 Pages: 185 - 195

    

36. Pure shift amide detection in conventional and TROSY-type experiments of ¹³C,¹⁵N-labeled proteins

    • Jens D. Haller
    • Andrea Bodor
    • Burkhard Luy

    Article Open access 18 November 2022 Pages: 213 - 221

    

37. Optimizing frequency sampling in CEST experiments

    • Nicolas Bolik-Coulon
    • D. Flemming Hansen
    • Lewis E. Kay

    Article 04 October 2022 Pages: 167 - 183

    

38. Characterization of conformational heterogeneity via higher-dimensionality, proton-detected solid-state NMR

    • Ekaterina Burakova
    • Suresh K. Vasa
    • Rasmus Linser

    Article Open access 23 September 2022 Pages: 197 - 212

    

39. Correction to: Validation of protein backbone structures calculated from NMR angular restraints using Rosetta

    • Joel Lapin
    • Alexander A. Nevzorov

    Correction 27 August 2022 Pages: 165 - 165

40. Measurement of ¹H^α transverse relaxation rates in proteins: application to solvent PREs

    • Yuki Toyama
    • Atul Kaushik Rangadurai
    • Lewis E. Kay

    Article 26 August 2022 Pages: 137 - 152

    

41. The measurement of binding affinities by NMR chemical shift perturbation

    • Billy Hobbs
    • Jack Drant
    • Mike P. Williamson

    Article Open access 03 August 2022 Pages: 153 - 163

    

42. Proton TOCSY NMR relaxation rates quantitate protein side chain mobility in the Pin1 WW domain

    • Gaddafi I. Danmaliki
    • Peter M. Hwang

    Article Open access 21 July 2022 Pages: 121 - 135

    

43. Distinct stereospecific effect of chiral tether between a tag and protein on the rigidity of paramagnetic tag

    • Jia-Liang Chen
    • Bin Li
    • Xun-Cheng Su

    Article 16 July 2022 Pages: 107 - 119

    

44. Metabolic¹⁵N labeling of the N-glycosylated immunoglobulin G1 Fc with an engineered Saccharomyces cerevisiae strain

    • Anjali Shenoy
    • Alexander R. Davis
    • Adam W. Barb

    Article 08 July 2022 Pages: 95 - 105

    

45. The time-zero HSQC method improves the linear free energy relationship of a polypeptide chain through the accurate measurement of residue-specific equilibrium constants

    • Seiichiro Hayashi
    • Daisuke Kohda

    Article 14 June 2022 Pages: 87 - 94

    

46. Towards autonomous analysis of chemical exchange saturation transfer experiments using deep neural networks

    • Gogulan Karunanithy
    • Tairan Yuwen
    • D. Flemming Hansen

    Article Open access 27 May 2022 Pages: 75 - 86

    

47. Ligand-induced structural transitions combined with paramagnetic ions facilitate unambiguous NMR assignments of methyl groups in large proteins

    • Lars Mühlberg
    • Tuncay Alarcin
    • Alvaro Mallagaray

    Article Open access 10 April 2022 Pages: 59 - 74

    

48. Fundamental and practical aspects of machine learning for the peak picking of biomolecular NMR spectra

    • Da-Wei Li
    • Alexandar L. Hansen
    • Rafael Brüschweiler

    Perspective Open access 07 April 2022 Pages: 49 - 57

    

49. ⁷⁷Se-¹³C based dipolar correlation experiments to map selenium sites in microcrystalline proteins

    • Caitlin M. Quinn
    • Shiping Xu
    • Sharon Rozovsky

    Communication 23 March 2022 Pages: 29 - 37

    

50. Journal of Biomolecular NMR: Editorial

    • Art Palmer

    Editorial 22 March 2022 Pages: 1 - 1