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Effect of hydrocarbons and other parameters on hydrocarbon-utilizing Pichia angusta MTCC-225

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Abstract

Pichia angusta MTCC-225, a catalase-positive yeast that utilizes methanol and lighter hydrocarbons, is the subject of this investigation. An orthogonal experimental design L16 was used to investigate the effects of methanol, a gas mixture, zero air, temperature, agitation, and salts solution on hydrocarbon utilizing P. angusta. QUALITEK-4 Software was used for automatic design and analysis of the experimental results. Among the various parameters tested, agitation contributed the highest influence (56.5%). Zero air, methanol concentration, and gas mixture showed a moderate influence on the growth of P. angusta. Methanol concentration and gas mixture showed a 10.91 and 10.12% influence, respectively, on yeast growth. Zero air played an important role, with a 15.19% influence on the utilization of hydrocarbon.

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Author notes

  1. R. Sreenivas Rao

    Present address: Centre for Cellular and Molecular Biology, Room no. W113, West Wing Ground Floor, Uppal Road, 500007, Hyderabad, India

Authors and Affiliations

  1. National Geophysical Research Institute, Uppal Road, 500007, Hyderabad, India

    R. Sreenivas Rao, M. A. Rasheed, G. Kalpana, D. J. Patil & B. Kumar

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  1. R. Sreenivas Rao
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  2. M. A. Rasheed
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  3. G. Kalpana
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  4. D. J. Patil
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  5. B. Kumar
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Correspondence to R. Sreenivas Rao.

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Sreenivas Rao, R., Rasheed, M.A., Kalpana, G. et al. Effect of hydrocarbons and other parameters on hydrocarbon-utilizing Pichia angusta MTCC-225. Appl Biochem Biotechnol 126, 205–214 (2005). https://doi.org/10.1385/ABAB:126:3:205

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  • DOI: https://doi.org/10.1385/ABAB:126:3:205

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