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Neutron reflectivity of supported membranes incorporating terminally anchored polymers: Protrusions vs. blisters

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Abstract.

The effect of terminally anchored chains on the structure of lipid bilayers adsorbed at the solid/water interface was characterized by neutron reflectivity. In the studied system, the inner leaflet, closer to the substrate, consisted of head-deuterated 1,2-distearoyl-sn-glycero-3-phosphorylcholine (DSPC) and the outer leaflet comprised a mixture of DSPC and polyethylene glycol (PEG) functionalized 1,2-distearoyl-sn-glycero-3-phosphoethanolamine. The DSPC headgroups were deuterated to enhance sensitivity and demarcate the bilayer/water interface. The effect on the inner and outer headgroup layers was characterized by w 1/2 , the width at half-height of the scattering length density profile. The inner headgroup layer was essentially unperturbed while w 1/2 of the outer layer increased significantly. This suggests that the anchored PEG chains give rise to headgroup protrusions rather than to blister-like membrane deformations.

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Authors and Affiliations

  1. Institute Max von Laue - Paul Langevin, 6 rue Jules Horowitz, BP 156, 38042, Grenoble, France

    G. Fragneto

  2. Université de Grenoble 1/CNRS, LIPhy UMR 5588, BP 87, 38041, Grenoble, France

    A. Halperin

  3. Department of Physics, Chemistry and Pharmacy, and MEMPHYS, Center for Biomembrane Physics, University of Southern Denmark, Campusvej 55, 5230, Odense M, Denmark

    B. Klösgen

  4. Département de Physique, Faculté des Sciences, Université Libre de Bruxelles, Boulevard du Triomphe, 1050, Bruxelles, Belgium

    M. Sferrazza

Authors
  1. G. Fragneto
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  2. A. Halperin
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  3. B. Klösgen
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  4. M. Sferrazza
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Correspondence to G. Fragneto.

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Fragneto, G., Halperin, A., Klösgen, B. et al. Neutron reflectivity of supported membranes incorporating terminally anchored polymers: Protrusions vs. blisters. Eur. Phys. J. E 36, 3 (2013). https://doi.org/10.1140/epje/i2013-13003-6

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  • DOI: https://doi.org/10.1140/epje/i2013-13003-6

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