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Action spectroscopy of isomer-selected luciferin anions

  • Regular Article - Molecular Physics and Chemical Physics
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Abstract

Luciferin molecules are common luminophores found throughout the biological kingdoms. Here, electrospray ionization and tandem ion mobility spectrometry coupled with laser spectroscopy are used to demonstrate that D-luciferin and oxyluciferin deprotonated anions can be produced in two isomeric forms, which can be separated by virtue of their different collision cross sections with a buffer gas. The two isomers possess distinguishable but partially overlapping photodepletion action spectra over the visible range, implying distinct intrinsic absorption profiles. The site of deprotonation and tautomeric forms of the electrosprayed isomers are assigned through comparisons between experimental and calculated collision cross sections and electronic excitation energies. The study clearly shows that electrospray ionization of biochromophore molecules can generate multiple isomeric forms with distinct electronic spectra.

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Data Availability Statement

This manuscript has data included as electronic supplementary material.

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Acknowledgements

This research was supported under the Australian Research Council’s Discovery Project funding scheme (DP150101427 and DP160100474). Electronic structure calculations were carried out on the High Performance Computing Cluster supported by the Research and Specialist Computing Support service at the University of East Anglia. CK thanks Augustinus Fonden, Niels Bohr Fondet and Oticon Fonden for travel support. EC acknowledges support by the Austrian Science Fund (FWF) through a Schrödinger Fellowship (Nr. J4013-N36).

Author information

Authors and Affiliations

  1. Department of Physics and Astronomy, Aarhus University, 8000, Aarhus, Denmark

    Christina Kjær & Steen Brøndsted Nielsen

  2. School of Chemistry, Norwich Research Park ,University of East Anglia, Norwich, NR4 7TJ, UK

    James N. Bull

  3. Bruker Daltonik GmbH, Fahrenheitstrasse 4, 28359, Bremen, Germany

    Eduardo Carrascosa

  4. School of Chemistry, University of Melbourne, Parkville, VIC, 3010, Australia

    Evan J. Bieske

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  1. Christina Kjær
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  2. James N. Bull
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  4. Steen Brøndsted Nielsen
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  5. Evan J. Bieske
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Contributions

Action spectroscopy experiments were performed by CK, JNB and EC in the laboratory of EJB. Calculations were performed by CK and JNB. The manuscript was drafted by JNB with contributions from all authors.

Corresponding author

Correspondence to Evan J. Bieske.

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Kjær, C., Bull, J.N., Carrascosa, E. et al. Action spectroscopy of isomer-selected luciferin anions. Eur. Phys. J. D 75, 72 (2021). https://doi.org/10.1140/epjd/s10053-021-00076-w

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  • DOI: https://doi.org/10.1140/epjd/s10053-021-00076-w

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