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Development and evaluation of STS diagnostic marker to track turmeric (Curcuma longa L.) resistance against rhizome rot caused by Pythium aphanidermatum

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Abstract

The entire spectrum of cultivated turmeric germplasm available today is highly susceptible to rhizome rot disease caused by necrotrophic oomycete Pythium aphanidermatum. Conventional host resistance breeding approaches have not been very successful in the crop owing to its lack of genetic variability, obligatory asexual nature and stigmatic incompatibility. The present study sought to develop easy, rapid and reliable molecular markers to identify new turmeric germplasm that could be used as source of resistance to P. aphanidermatum. Bulk segregant analyses using pooled DNA from resistant and susceptible genotypes with 40 inter simple sequence markers resulted in the isolation of a putative resistance specific marker ClRSM. The resistance specific fragment was converted into a sequence tagged marker ClSTS, which could successfully amplify a 720 bp, fragment in resistance turmeric genotypes but not in the susceptible plants. Southern blotting confirmed it as a single copy locus found associated with the resistant genotypes. Further, the ClSTS marker precisely identified 10 resistant and five susceptible genotypes among the 15 turmeric germplasm with unknown disease response collected from different agroclimatic regions. Inoculation assessment of the 15 individuals with a virulent strain of P. aphanidermatum corroborated with the STS marker results. Hence, the identified STS marker can be use as an efficient tool for early and rapid identification of new turmeric cultivars resistant to rhizome rot through marker-assisted selection.

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Abbreviations

ClRSM:

Curcuma longa resistance specific marker

ISSR:

Inter simple sequence repeats

STS:

Sequence tagged sites

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Acknowledgement

BK gratefully acknowledges the financial assistance in the form of senior research fellowship from the Council of Scientific and Industrial Research, Government of India, New Delhi. The authors are thankful to the President, Siksha O Anusandhan University for his guidance and support and grateful to DST-FIST, Govt. of India, for the facilities provided to Centre of Biotechnology, Siksha O Anusandhan University.

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Authors and Affiliations

  1. Functional Genomics Lab, Centre of Biotechnology, Siksha O Anusandhan University, Bhubaneswar, 751003, India

    Basudeba Kar, Sanghamitra Nayak & Raj Kumar Joshi

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  1. Basudeba Kar
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  2. Sanghamitra Nayak
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  3. Raj Kumar Joshi
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Correspondence to Raj Kumar Joshi.

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Kar, B., Nayak, S. & Joshi, R.K. Development and evaluation of STS diagnostic marker to track turmeric (Curcuma longa L.) resistance against rhizome rot caused by Pythium aphanidermatum . Australasian Plant Pathol. 43, 167–175 (2014). https://doi.org/10.1007/s13313-013-0259-2

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