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Single or Combined Applications of Zinc and Multi-strain Probiotic on Intestinal Histomorphology of Broilers Under Cyclic Heat Stress

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Abstract

Two-hundred-eighty-day-old broiler chicks were divided into seven groups. The groups were designated as T1, thermoneutral zone; T2, heat stressed (HS); T3, HS + zinc (Zn) supplementation (30 mg/kg); T4, HS + Zn (60 mg/kg); T5, HS + probiotic (0.1 g/kg); T6, HS + probiotic (0.1 g/kg) + Zn (30 mg/kg); and T7, HS + Zn (60 mg/kg) + probiotic (0.1 g/kg). Significant decrease (p < 0.05) was observed in villus height (VH), VH to crypt depth ratio, and villus surface area of all intestinal segments in the T2 group when compared with the T1 group. The same parameters had significantly higher (p < 0.05) values in the jejunum and ileum of the Zn- and probiotic-supplemented groups (alone + combination) when compared with the T2 group. The birds exposed to HS showed fewer (p < 0.05) intraepithelial lymphocytes (IELs) in the jejunum and ileum than the T1 group, while their count increased in the jejunum and ileum with dietary treatments. In conclusion, Zn and probiotic positively modulated the intestinal microstructures of broilers kept under high environmental temperature.

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Acknowledgments

We acknowledge the Department of Poultry Production, University of Veterinary and Animal Sciences Lahore, Pakistan, for providing experimental shed and other facilities for the conduction of experimental trial.

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Authors and Affiliations

  1. Department of Animal Health, Faculty of Animal Husbandry and Veterinary Sciences, University of Agriculture, Peshawar, Pakistan

    Muqader Shah & Rifat Ullah Khan

  2. Department of Anatomy and Histology, Faculty of Biosciences, University of Veterinary and Animal Sciences, Lahore, Pakistan

    Hafsa Zaneb, Saima Masood, Imad Khan & Salahud Din

  3. Department of Animal Nutrition, Faculty of Animal Husbandry & Veterinary Science, The University of Agriculture, Peshawar, Pakistan

    Muhammad Mobashar

  4. Department of Bio-Sciences, Gomal College of Veterinary Sciences, Gomal University, D. I. Khan, Pakistan

    Muhammad Shoaib Khan

  5. Department of Physiology, Faculty of Biosciences, University of Veterinary and Animal Sciences, Lahore, Pakistan

    Habib Ur Rehman

  6. Department of Veterinary Medicine, University of Bari “Aldo Moro”, Valenzano, BA, Italy

    Antonella Tinelli

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  1. Muqader Shah
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  2. Hafsa Zaneb
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Contributions

MS, HZ, SM, and HR contributed in experimental design, data analysis, and manuscript writing. MS, HZ, IK, and SD contributed in sample processing, data acquisition, and interpretation. MS, RK, IA, and MSK contributed in manuscript preparation.

Corresponding author

Correspondence to Muqader Shah.

Ethics declarations

For conducting this experiment, proper ethical consent was taken from the Ethical Review Committee, University of Veterinary and Animal Sciences Lahore, Pakistan.

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The authors declare that they have no conflict of interest.

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Shah, M., Zaneb, H., Masood, S. et al. Single or Combined Applications of Zinc and Multi-strain Probiotic on Intestinal Histomorphology of Broilers Under Cyclic Heat Stress. Probiotics & Antimicro. Prot. 12, 473–480 (2020). https://doi.org/10.1007/s12602-019-09561-6

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  • DOI: https://doi.org/10.1007/s12602-019-09561-6

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