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Analysis of a draft genome sequence of Kitasatospora cheerisanensis KCTC 2395 producing bafilomycin antibiotics

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Abstract

Kitasatospora cheerisanensis KCTC 2395, producing bafilomycin antibiotics belonging to plecomacrolide group, was isolated from a soil sample at Mt. Jiri, Korea. The draft genome sequence contains 8.04 Mb with 73.6% G+C content and 7,810 open reading frames. All the genes for aerial mycelium and spore formations were confirmed in this draft genome. In phylogenetic analysis of MurE proteins (UDP-N-acetylmuramyl-L-alanyl-D-glutamate:DAP ligase) in a conserved dcw (division of cell wall) locus, MurE proteins of Kitasatospora species were placed in a separate clade between MurEs of Streptomyces species incorporating LL-diaminopimelic acid (DAP) and MurEs of Saccharopolyspora erythraea as well as Mycobacterium tuberculosis ligating meso-DAP. From this finding, it was assumed that Kitasatospora MurEs exhibit the substrate specificity for both LL-DAP and meso-DAP. The bafilomycin biosynthetic gene cluster was located in the left subtelomeric region. In 71.3 kb-long gene cluster, 17 genes probably involved in the biosynthesis of bafilomycin derivatives were deduced, including 5 polyketide synthase (PKS) genes comprised of 12 PKS modules.

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Authors and Affiliations

  1. College of Pharmacy, Yeungnam University, Gyongsan, 712-749, Republic of Korea

    Jae Yoon Hwang, Soo Hee Kim, Hye Ryeung Oh & Doo Hyun Nam

  2. Institute for Drug Development, Yeungnam University, Gyongsan, 712-749, Republic of Korea

    Eunju Kwon

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  1. Jae Yoon Hwang
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  2. Soo Hee Kim
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  3. Hye Ryeung Oh
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  4. Eunju Kwon
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  5. Doo Hyun Nam
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Correspondence to Doo Hyun Nam.

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Hwang, J.Y., Kim, S.H., Oh, H.R. et al. Analysis of a draft genome sequence of Kitasatospora cheerisanensis KCTC 2395 producing bafilomycin antibiotics. J Microbiol. 53, 84–89 (2015). https://doi.org/10.1007/s12275-015-4340-0

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  • DOI: https://doi.org/10.1007/s12275-015-4340-0

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