Abstract
Burkholderia cenocepacia TAtl-371 was isolated from the rhizosphere of a tomato plant growing in Atlatlahucan, Morelos, Mexico. This strain exhibited a broad antimicrobial spectrum against bacteria, yeast, and fungi. Here, we report and describe the improved, high-quality permanent draft genome of B. cenocepacia TAtl-371, which was sequenced using a combination of PacBio RS and PacBio RS II sequencing methods. The 7,496,106 bp genome of the TAtl-371 strain is arranged in three scaffolds, contains 6722 protein-coding genes, and 99 RNA only-encoding genes. Genome analysis revealed genes related to biosynthesis of antimicrobials such as non-ribosomal peptides, siderophores, chitinases, and bacteriocins. Moreover, analysis of bacterial growth on different carbon and nitrogen sources shows that the strain retains its antimicrobial ability.
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Acknowledgements
FURR, EYTG, and IAH recipients of a fellowship from CONACYT. JAIG and PES are recipient of SNI, EDI, and COFAA fellowships. We thank Dr. E.O. Lopez-Villegas (Escuela Nacional de Ciencias Biológicas, IPN) for the transmission electron microscopic analysis. The genome sequence was conducted by the U.S. Department of Energy, Joint Genome Institute, a DOE Office of Science User Facility, is supported by the Office of Sciences of the U.S. Department of Energy under the proposal 1572 and Contract No. DE-AC02-05CH11231. Phenotypic analysis was partially funded by Projects SIP 20170492 and SIP 20180117.
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Nucleotide Sequence Accession Numbers
The Whole Genome project has been deposited into GenBank under the accession no. PRJEB16032. The version described in this paper is version PRJEB16032.
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Rojas-Rojas, F.U., Sánchez-López, D., Tapia-García, E.Y. et al. Draft Genome of Burkholderia cenocepacia TAtl-371, a Strain from the Burkholderia cepacia Complex Retains Antagonism in Different Carbon and Nitrogen Sources. Curr Microbiol 76, 566–574 (2019). https://doi.org/10.1007/s00284-019-01657-0
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DOI: https://doi.org/10.1007/s00284-019-01657-0