Skip to main content
SpringerLink
Log in

Inorganic Vanadium Supplementation in Crossbred Calves: Effects on Antioxidant Status, Immune Response and Haemato-Biochemical Attributes

  • Published:
Biological Trace Element Research Aims and scope Submit manuscript

Abstract

The aim of the study was to assess the effect of inorganic vanadium (V) supplementation on antioxidant enzymes, immune status, and haemato-biochemical attributes of growing crossbred calves. Twenty-four male Karan Fries calves (Tharparkar × Holstein Friesian) (initial body mass 72.83 ± 2.5 kg; age 3–9 month) were randomly allocated to four groups: the control (received basal diet devoid of supplemental V), the 3 ppm (received basal diet with 3 mg/kg V), the 6 ppm (received basal diet with 6 mg/kg V) and the 9 ppm group (received basal diet with 9 mg/kg V). All the calves were fed for 150 days as per ICAR (2013) feeding standards to meet their nutrient requirements for 500 g growth rate/day. Peripheral blood samples were collected at the start of experiment and subsequently at 30, 60, 90, 120 and 150 days post-V supplementation for determination of antioxidant enzyme activity, immunological parameters and haemato-biochemical attributes. Results indicated that dietary supplementation of V did not affect daily gain, feed intake and haematological parameters. Crossbred calves fed with 9 mg V/kg diet showed reduced (P < 0.05) plasma total cholesterol concentration; however, plasma total protein and glucose concentration remained unaltered. Glutathione peroxidase (GPx) activity as well as immunoglobulin G (IgG) concentration was significantly (P < 0.05) higher in group supplemented with 9 mg V/kg DM; however, superoxide dismutase (SOD), catalase activity and total plasma immunoglobulin (Ig) concentration were similar in all experimental group. Dietary V supplementation showed a negative relation with plasma thiobarbituric acid reactive substances (TBARS) concentration, whereas non-esterified fatty acid (NEFA) concentration remained unaltered among all groups. Plasma V level increased (P < 0.05) with increasing dietary V levels without affecting levels of Ca, Mg, Fe, Cu and Zn. In conclusion, a dietary addition of 9 mg V/kg DM reduced cholesterol content and improved antioxidant and immune response in growing crossbred calves.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Underwood EJ (1971) Trace elements in human and animal nutrition, 3rd edn. Academic Press, Inc, New York

    Google Scholar 

  2. Nielsen FH (2000) Importance of making dietary recommendations for elements designated as nutritionally beneficial, pharmacologically beneficial, or conditionally essential. J Trace Elem Exp Med 13:113–129

    Article  CAS  Google Scholar 

  3. Verma S, Cam MC, McNeil JH (1998) Nutritional factors that can favorably influence the glucose/insulin system, vanadium. J Am Coll Nutr 17(1):11–18

    Article  CAS  Google Scholar 

  4. Harland BF, Harland Williams BA (1994) Is vanadium of human nutritional importance yet? J Am Diet Assoc 94(8):891–894

    Article  CAS  Google Scholar 

  5. Anke M, Groppel B, Kronemann H, Führer E (1983) Influence of vanadium deficiency on growth, reproduction and life expectancy of female goats. Mengen- und Spurenelemente 3:135–141

    Google Scholar 

  6. Francik R, Krosniak M, Barlik M, Kudla A, Grybos R, Librowski T (2011) Impact of vanadium complexes treatment on the oxidative stress factors in Wistar rats plasma. Bioinorg Chem Appl Article ID 206316:1–8

    Google Scholar 

  7. Harati M, Ani M (2006) Low doses of vanadyl sulfate protects rats from lipid peroxidation and hypertriglyceridemic effects of fructose-enriched diet. Int J Diabetes Metab 14(3):134

    CAS  Google Scholar 

  8. Kim AD, Zhang R, Kang KA, You HJ, Hyun JW (2011) Increased glutathione synthesis following Nrf2 activation by vanadyl sulfate in human chang liver cells. Int J Mol Sci 12(12):8878–8894

    Article  CAS  Google Scholar 

  9. Menon AS, Rau M, Ramasarma T, Crane FL (1980) Vanadate inhibits mevalonate synthesis and activates NADH oxidation in microsomes. FEBS Lett 114(1):139–141

    Article  CAS  Google Scholar 

  10. Wang J, Jin GM, Gu YF, LI SH, GU SY (2006) Effects of vanadium on development of immune organs in broilers. Vet Sci China 7:14

    Google Scholar 

  11. Alexandrova R, Alexandrov I, Nikolova E (2002) Effect of orally administered ammonium vanadate on the immune response of experimental animals. C R Acad Bulg Sci 55(3):3–69

    Google Scholar 

  12. Anke M, Groppel B, Gruhn K, Kosla T, Szilagyi M (1986b) New research on vanadium deficiency in ruminants. In: Proceedings 5th Spurenelement symposium, Jena, University Jena, Germany, pp 1266–1275

  13. Levina A, McLeod AI, Pulte A, Aitken JB, Lay PA (2015) Biotransformations of antidiabetic vanadium prodrugs in mammalian cells and cell culture media: a XANES spectroscopic study. Inorg Chem 54(14):6707–6718

    Article  CAS  Google Scholar 

  14. Poucheret P, Verma S, Grynpas MD, McNeill JH (1998) Vanadium and diabetes. Mol Cell Biochem 188(1):73–80

    Article  CAS  Google Scholar 

  15. Cantley LC, Josephson L, Warner R, Yanagisawa M, Lechene C, Guidotti G (1977) Vanadate is a potent (Na,K)-ATPase inhibitor found in ATP derived from muscle. J Biol Chem 252(21):7421–7423

    CAS  PubMed  Google Scholar 

  16. Krejsa CM, Nadler SG, Esseltyn JM, Kavanagh TJ, Ledbetter JA, Schieven GL (1997) Role of oxidative stress in the action of vanadium phosphotyrosine phosphatase inhibitors. Redox independent activation of NF-kappaB. J Biol Chem 272(17):11541–11549

    Article  CAS  Google Scholar 

  17. Pal RP, Mani V, Tripathi D, Kumar R, Kewalramani NJ (2017) Influence of feeding inorganic vanadium on growth performance, endocrine variables and biomarkers of bone health in crossbred calves. Biol Trace Elem Res 1–9

  18. Indian council of Agricultural Research (2013) Nutrient requirements of cattle and buffalo. Nutrient requirements of animals, New Delhi, India

  19. Association of Official Analytical Chemists (2005) Official methods of analysis. 18th edn. Washington DC, USA

  20. Van Soest PV, Robertson JB, Lewis BA (1991) Methods for dietary fibre, neutral detergent fibre, and nonstarch polysaccharides in relation to animal nutrition. J Dairy Sci 74(10):3583–3597

    Article  Google Scholar 

  21. NRC (2001) Nutrient requirements of dairy cattle, 7th revised edition. National Academy Press, Washington, DC

    Google Scholar 

  22. Madesh M, Balasubramanian KA (1998) Microtiter plate assay for superoxide dismutase using MTT reduction by superoxide. Indian J Biochem Biophys 35(3):184–188

    CAS  Google Scholar 

  23. Aebi H (1984) Catalase in vitro. Methods Enzymol 105:121–126

    Article  CAS  Google Scholar 

  24. Paglia DE, Valentine WN (1967) Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 70(1):158–169

    CAS  Google Scholar 

  25. Niehaus WG, Samuelsson B (1968) Formation of malonaldehyde from phospholipid arachidonate during microsomal lipid peroxidation. Eur J Biochem 6(1):126–130

    Article  CAS  Google Scholar 

  26. Kaushal D, Kansal V (2012) Probiotic Dahi containing and alleviates age-inflicted oxidative stress and improves expression of biomarkers of ageing in mice. Mol Biol Rep 2(39):1791–1799

    Article  Google Scholar 

  27. McEwan AD, Fisher EW, Selman IE, Penhale WJ (1970) A turbidity test for the estimation of immune globulin levels in neonatal calf serum. Clin Chim Acta 27(1):155–163

    Article  CAS  Google Scholar 

  28. Heidari SR, Ganjkhanlou M, Zali A, Ghorbani GR, Dehghan-Banadaky M, Hayirli A (2016) Effects of vanadium supplementation on performance and metabolic parameters in periparturient dairy cows. Anim Feed Sci Technol 216:138–145

    Article  CAS  Google Scholar 

  29. Yamaguchi M, Oishi H, Suketa Y (1989) Effect of vanadium on bone metabolism in weanling rats: zinc prevents the toxic effect of vanadium. Res Exp Med 189(1):47–53

    Article  CAS  Google Scholar 

  30. Omer HAA, Abdel-Magid SS, EL-Nomeary YAA, Nassar SA, Nasr SM, Abou-Zeina HA (2015) Nutritional impact of partial replacement of cotton seed meal with distillers dried grain with solubles (DDGS) on animal performance, digestion coefficients and some blood constituents in crossbred calves. World Appl Sci J 33(4):580–589

    CAS  Google Scholar 

  31. Brar RS, Sandhu HS, Singh A (2002) Veterinary clinical diagnosis by laboratory methods. Kalyani Publishers, Ludhiana

    Google Scholar 

  32. Mohri M, Sarrafzadeh F, Seifi HA, Farzaneh N (2004) Effects of oral iron supplementation on some haematological parameters and iron biochemistry in neonatal dairy calves. Comp Clin Path 13(2):39–42

    Article  CAS  Google Scholar 

  33. Dai S, Vera E, McNeill JH (1995) Lack of haematological effect of oral vanadium treatment in rats. Pharmacol Toxicol 76:263–268

    Article  CAS  Google Scholar 

  34. Zaporowska H, Wasilewski W (1992) Combined effect of vanadium and zinc on certain selected haematological indices in rats. Comp Biochem Physiol C 103(1):143–147

    Article  CAS  Google Scholar 

  35. Fawcett JP, Farquhar SJ, Thou T, Shand BI (1997) Oral vanadyl sulphate does not affect blood cells, viscosity or biochemistry in humans. Pharmacol Toxicol 80(4):202–206

    Article  CAS  Google Scholar 

  36. Preet A, Gupta BL, Yadava PK, Baquer NZ (2005) Efficacy of lower doses of vanadium in restoring altered glucose metabolism and antioxidant status in diabetic rat lenses. J Biosci 30(2):221–230

    Article  CAS  Google Scholar 

  37. Upreti J, Ali S, Basir SF (2014) Lower doses of vanadate in combination with Azadirachta indica leaf extract restore altered antioxidant status in the brain of streptozotocin induced diabetic rats. World J Pharm Pharm Sci 4(1):1347–1358

    Google Scholar 

  38. Elshaari FA, Hadad G, Barassi IF (2011) Effect of sodium vanadate on liver function of experimental rats. J Basic Med Allied Sci 1:5–10

    Google Scholar 

  39. Paramanik D, Rajalakshmi MG (2013) In-vivo evaluation of potential toxicity of vanadium pentoxide in male wistar rats. Int J Appl Biol Pharm 4(3):260–271

    CAS  Google Scholar 

  40. Shahi MM, Haidari F, Shiri MR (2011) Comparison of effect of resveratrol and vanadium on diabetes related dyslipidemia and hyperglycemia in streptozotocin induced diabetic rats. Adv Pharm Bull 1(2):81–86

    Google Scholar 

  41. Mandal GP, Dass RS (2010) Haemato-biochemical profile of crossbred calves supplemented with inorganic and organic source of zinc. Indian J Anim Res 44(3):197–200

    Google Scholar 

  42. Niwas R, Singh D, Paswan V, Bisen B, Albial MA (2012) Herbal drugs and their effect on biochemical attributes of crossbred calves. Bioscan 7(4):665–667

    CAS  Google Scholar 

  43. Xie M, Chen D, Zhang F, Willsky GR, Crans DC, Ding W (2014) Effects of vanadium (III, IV, V)-chlorodipicolinate on glycolysis and antioxidant status in the liver of STZ-induced diabetic rats. J Inorg Biochem 136:47–56

    Article  CAS  Google Scholar 

  44. Tsave O, Petanidis S, Kioseoglou E, Yavropoulou MP, Yovos JG, Anestakis D, Salifoglou A (2016) Role of vanadium in cellular and molecular immunology: association with immune-related inflammation and pharmacotoxicology mechanisms. Oxidative Med Cell Longev 2016:4013639

    Article  Google Scholar 

  45. Ha D, Joo H, Ahn G, Kim MJ, Bing SJ, An S, Jee Y (2012) Jeju ground water containing vanadium induced immune activation on splenocytes of low dose γ-rays-irradiated mice. Food Chem Toxicol 50(6):2097–2105

    Article  CAS  Google Scholar 

  46. Cui W, Cui HM, Peng X, Zuo Z, Liu X, Wu B (2011) Effect of vanadium on the subset and proliferation of peripheral blood T cells, and serum interleukin-2 content in broilers. Biol Trace Elem Res 141(1–3):192–199

    Article  CAS  Google Scholar 

  47. Suman M, Kaur H (2015) Effect of positive dietary cation anion difference (DCAD) diet on blood biochemical and immunological parameters in crossbred calves in winter. Indian J Anim Sci 85:462–468

    CAS  Google Scholar 

  48. Kumar M, Kaur H, Deka RS, Mani V, Tyagi AK, Chandra G (2015) Dietary inorganic chromium in summer-exposed buffalo calves (Bubalus bubalis): effects on biomarkers of heat stress, immune status, and endocrine variables. Biol Trace Elem Res 167(1):18–27

    Article  CAS  Google Scholar 

  49. Qureshi MA, Hill CH, Heggen CL (1999) Vanadium stimulates immunological responses of chicks. Vet Immunol Immunopathol 68(1):61–71

    Article  CAS  Google Scholar 

  50. Preuss HG, Jarrell ST, Scheckenbach R, Lieberman S, Anderson RA (1998) Comparative effects of chromium, vanadium and Gymnema sylvestre on sugar-induced blood pressure elevations in SHR. J Am Coll Nutr 17(2):116–123

    Article  CAS  Google Scholar 

  51. El Karib AO, Al-Ani B, Al-Hashem F, Dallak M, Bin-Jaliah I, El-Gamal B, Haidara MA (2016) Insulin and vanadium protect against osteoarthritis development secondary to diabetes mellitus in rats. Arch Physiol Biochem 122(3):148–154

    Article  Google Scholar 

  52. White MF, Kahn CR (1994) The insulin signaling system. J Biol Chem 269:1–5

    CAS  PubMed  Google Scholar 

  53. Akhtar MS, Farooq AA, Mushtaq M (2009) Serum trace minerals variation during pre and post-portum period in Nilli-Ravi buffaloes. J Anim Plant Sci 19(4):182–184

    Google Scholar 

  54. Underwood EJ, Suttle NF (1983) Los minerales en la nutrición del ganado.UNDmE 2a, ed. Acribia, No. 636.084

Download references

Acknowledgments

The authors would like to thank the staff of the Animal Nutrition Division and Cattle Yard, Karnal, ICAR-NDRI, India. This study was funded by the Indian Council of Agricultural Research, New Delhi, India.

Author information

Authors and Affiliations

  1. Animal Nutrition Division, ICAR-National Dairy Research Institute, Karnal, 132001, India

    Ravi Prakash Pal, Veena Mani, Deepika Tripathi & Chander Datt

Authors
  1. Ravi Prakash Pal
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Veena Mani
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Deepika Tripathi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Chander Datt
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Veena Mani.

Ethics declarations

Conflict of Interest

The authors declare that they have no conflict of interest.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Pal, R.P., Mani, V., Tripathi, D. et al. Inorganic Vanadium Supplementation in Crossbred Calves: Effects on Antioxidant Status, Immune Response and Haemato-Biochemical Attributes. Biol Trace Elem Res 186, 154–161 (2018). https://doi.org/10.1007/s12011-018-1295-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12011-018-1295-0

Keywords

Search

Navigation