Abstract
Growth and fermentation characteristics, biomass composition, lipid characterization and metabolic profiling analysis of two different Schizochytrium sp. strains, the original strain and the industrial adaptive strain, were investigated in the fed-batch fermentation process. The final cell biomass, total lipids content, docosahexanoic acid (DHA) content and DHA productivity of the adaptive strain were much higher than those of the original strain. The metabolic distinctions which extensively existed between these two strains were revealed by the score plot of principal component analysis. In addition, potential biomarkers responsible for discriminating different strains were identified as myo-inositol, histidine, alanine, asparagine, cysteine, and oxalic acid. These findings provided new insights into the industrial strain screening and further improvement of DHA production by Schizochytrium sp.
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Acknowledgments
This work was financially supported by the National Basic Research Program of China (no. 2011CBA00802), the Scientific Research Project for Post-graduate in Jiangsu Province (no. CXLX11_0366), the Natural Science Foundation of Jiangsu Province (no. BK2012424), National Science Foundation for Distinguished Young Scholars of China (No. 21225626), the National Science and Technology Pillar Program (no. 2011BAD23B03), and the National High Technology Research and Development Program of China (no. 2012AA021704).
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Liang Qu and Lu-Jing Ren contributed equally to this work.
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Qu, L., Ren, LJ., Li, J. et al. Biomass Composition, Lipid Characterization, and Metabolic Profile Analysis of the Fed-Batch Fermentation Process of Two Different Docosahexanoic Acid Producing Schizochytrium sp. Strains. Appl Biochem Biotechnol 171, 1865–1876 (2013). https://doi.org/10.1007/s12010-013-0456-z
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DOI: https://doi.org/10.1007/s12010-013-0456-z