Skip to main content
SpringerLink
Log in

Isolation and Characterization of a Novel Thraustochytrid-like Microorganism that Efficiently Produces Docosahexaenoic Acid

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

A thraustochytrid-like microorganism (strain 12B) was isolated from the mangrove area of Okinawa, Japan. On the basis of its ectoplasmic net structure and biflagellate zoospores we determined strain 12B to be a novel member of the phylum Labyrinthulomycota in the kingdom Protoctista. When grown on glucose/seawater at 28 °C, it had a lipid content of 58% with docosahexaenoic acid (DHA; 22:6 n−3) at 43% of the total fatty acids. It had a growth rate of 0.38 h−1. The DHA production rate of 2.8 ± 0.7 g l−1 day−1 is the highest value reported for any microorganism.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • T Aki K Hachida K Yoshinaga Y Katai T Yamasaki S Kawamoto T Kakizono T Yamaoka S Shigeta O Suzuki K Ono (2003) ArticleTitleThraustochytrid as a potential source of carotenoids J. Am. Oil. Chem. Soc. 80 789–794 Occurrence Handle1:CAS:528:DC%2BD3sXmvFGlsro%3D

    CAS  Google Scholar 

  • Barclay WR (1992) Process for the heterotrophic production of microbial products with high concentrations of omega−3 highly unsaturated fatty acids. United States Patent 5,130,242

  • J Bezard JP Blond A Bernard P Clouet (1994) ArticleTitleThe metabolism and availability of essential fatty acids in animal and human tissues Reprod. Nutr. Dev. 34 539–568 Occurrence Handle1:CAS:528:DyaK2MXktlSgsbg%3D Occurrence Handle7840871

    CAS  PubMed  Google Scholar 

  • EG Bligh WJ Dyer (1959) ArticleTitleA rapid method of total lipid extraction and purification Can. J. Biochem. Physiol. 37 911–917 Occurrence Handle1:CAS:528:DyaG1MXhtVSgt70%3D Occurrence Handle13671378

    CAS  PubMed  Google Scholar 

  • RC Gaver CC Sweeley (1965) ArticleTitleMethods for methanolysis of sphingolipids and direct determination of long-chain bases by gas chromatography J. Am. Oil. Chem. Soc. 42 294–298 Occurrence Handle1:STN:280:CCqD1czmvVA%3D Occurrence Handle14279115

    CAS  PubMed  Google Scholar 

  • I Gill R Valivety (1997) ArticleTitlePolyunsaturated fatty acids, part 1: occurrence, biological activities and applications Trends Biotechnol. 15 401–409 Occurrence Handle1:CAS:528:DyaK2sXmslCksL4%3D Occurrence Handle9351284

    CAS  PubMed  Google Scholar 

  • RH Haschemeyer RJ Meysers (1972) Negative staining MA Hayat (Eds) Principles and Techniques of Electron Microscopy NumberInSeriesVol. 2 Van Nostrand Reinhold Co New York 114–117

    Google Scholar 

  • R Kroes EJ Schaefer RA Squire GM Williams (2003) ArticleTitleA review of the safety of DHA45-oil Food Chem. Toxicol. 41 1433–1446 Occurrence Handle1:CAS:528:DC%2BD3sXntVGnsrc%3D Occurrence Handle12962995

    CAS  PubMed  Google Scholar 

  • Kyle DJ, Reeb SE, Sicotte VJ (1995) Infant formula and baby food containing docosahexaenoic acid obtained from dinoflagellates. United States Patent 5,397,591

  • TE Lewis PD Nichols TA McMeekin (1999) ArticleTitleThe biotechnological potential of thraustochytrids Mar. Biotechnol. 1 580–587 Occurrence Handle10.1007/PL00011813 Occurrence Handle1:CAS:528:DC%2BD3cXktFCitw%3D%3D Occurrence Handle10612683

    Article  CAS  PubMed  Google Scholar 

  • Y Orikasa A Yamada R Yu Y Ito T Nishida I Yumoto K Watanabe H Okuyama (2004) ArticleTitleCharacterization of the eicosapentaenoic acid biosynthesis gene cluster from Shewanella sp. strain SCRC-2738 Cell Mol. Biol. 50 625–630 Occurrence Handle1:CAS:528:DC%2BD2cXhtVSis7fO Occurrence Handle15565743

    CAS  PubMed  Google Scholar 

  • D Porter (1989) Phylum Labyrinthulomycota L Margulis (Eds) Handbook of Protoctists Jones & Bartlett Publishers Boston 388–398

    Google Scholar 

  • C Ratledge (2004) ArticleTitleFatty acid biosynthesis in microorganisms being used for single cell oil production Biochimie 86 807–815 Occurrence Handle10.1016/j.biochi.2004.09.017 Occurrence Handle1:CAS:528:DC%2BD2cXhtVKksLbK Occurrence Handle15589690

    Article  CAS  PubMed  Google Scholar 

  • L Sijtsma ME de Swaaf (2004) ArticleTitleBiotechnological production and applications of the ω−3 polyunsaturated fatty acid docosahexaenoic acid Appl. Microbiol. Biotechnol. 64 146–153 Occurrence Handle10.1007/s00253-003-1525-y Occurrence Handle1:CAS:528:DC%2BD2cXis1Gls7c%3D Occurrence Handle14740186

    Article  CAS  PubMed  Google Scholar 

  • A Singh OP Ward (1996) ArticleTitleProduction of high yields of docosahexaenoic acid by Thraustochytrium roseum ATCC 28210 J. Ind. Microbiol. 16 370–373 Occurrence Handle10.1007/BF01570118 Occurrence Handle1:CAS:528:DyaK28XkslSksrg%3D

    Article  CAS  Google Scholar 

  • ME Swaaf Particlede TC Rijk Particlede P Meer Particlevan der G Eggink L Sijtsma (2003) ArticleTitleAnalysis of docosahexaenoic acid biosynthesis in Crypthecodinium cohnii by 13C labeling and desaturase inhibitor experiments J. Biotechnol. 103 21–29 Occurrence Handle12770501

    PubMed  Google Scholar 

  • N Takakuwa M Kinoshita Y Oda M Ohnishi (2002) ArticleTitleExistence of cerebroside in Saccharomyces kluyveri and its related species FEMS Yeast Res. 2 533–538 Occurrence Handle1:CAS:528:DC%2BD38XovFGgsbs%3D Occurrence Handle12702269

    CAS  PubMed  Google Scholar 

  • R Vazhappilly F Chen (1998) ArticleTitleHeterotrophic production potential of omega−3 polyunsaturated fatty acids by microalgae and algae-like microorganisms Botanica Marina 41 553–558 Occurrence Handle1:CAS:528:DyaK1cXnvVahtL8%3D

    CAS  Google Scholar 

  • T Yaguchi S Tanaka T Yokochi T Nakahara T Higashihara (1997) ArticleTitleProduction of high yields of docosahexaenoic acid by Schizochytrium limacinum strain SR21 J. Am. Oil. Chem. Soc. 74 1431–1434 Occurrence Handle1:CAS:528:DyaK2sXnsFGrtr8%3D

    CAS  Google Scholar 

  • T Yokochi D Honda T Higashihara T Nakahara (1998) ArticleTitleOptimization of docosahexaenoic acid production by Schizochytrium limacinum SR21 Appl. Microbiol. Biotechnol. 49 72–76 Occurrence Handle10.1007/s002530051139 Occurrence Handle1:CAS:528:DyaK1cXhtVantbc%3D

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Environmental Molecular Biology, Graduate School of Environmental Earth Science, Hokkaido University, Sapporo, Kita-ku, 060-0810, Japan

    Zakia Perveen, Hitomi Ando, Akio Ueno & Hidetoshi Okuyama

  2. ROM Co. Ltd., Chuo-ku, Sapporo, 064-0804, Japan

    Yukiya Ito & Yusuke Yamamoto

  3. Department of Chemical and Biological Sciences, Faculty of Science, Japan Women’s University, 112-8681, Bunkyo-ku, Japan

    Yohko Yamada & Takako Kaneko

  4. Division of Biology, Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Meguro-ku, Tokyo, 153-8902, Japan

    Tomoko Takagi

  5. Division of Biological Sciences, Graduate School of Science, Hokkaido University, Kita-ku, Sapporo, 060-0810, Japan

    Kazuhiro Kogame

Authors
  1. Zakia Perveen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hitomi Ando
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Akio Ueno
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yukiya Ito
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yusuke Yamamoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yohko Yamada
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Tomoko Takagi
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Takako Kaneko
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Kazuhiro Kogame
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Hidetoshi Okuyama
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hidetoshi Okuyama.

Additional information

Received 7 October 2005; Revisions requested 7 October 2005; Revisions received 15 November 2005; Accepted 15 November 2005

Rights and permissions

Reprints and permissions

About this article

Cite this article

Perveen, Z., Ando, H., Ueno, A. et al. Isolation and Characterization of a Novel Thraustochytrid-like Microorganism that Efficiently Produces Docosahexaenoic Acid. Biotechnol Lett 28, 197–202 (2006). https://doi.org/10.1007/s10529-005-5335-4

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10529-005-5335-4

Keywords

Search

Navigation