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Antiproliferative and antioxidative activities of cuttlefish (Sepia pharaonis) protein hydrolysates as affected by degree of hydrolysis

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Abstract

Bioactivities (including antioxidative and antiproliferative properties) of cuttlefish mantle protein hydrolysates (CPH) with the degree of hydrolysis (DH) of 20.9, 25.5, 30.6, 35.3 and 40.6% (shortened as 20, 25, 30, 35 and 40%, respectively) prepared using alcalase were evaluated. The results indicated that the CPH with 20, 30 and 40% DH showed the greatest activity against DPPH radical scavenging [5.2 µmol TE (torolox equivalent)/g sample], reducing power (0.4 absorbance at 700 nm) and total antioxidant capacity (0.6 mg ascorbic acid equivalent/g sample), which were 2.5, 6.5 and 13.8 times higher than the cuttlefish mantle protein isolate (CPI), respectively. The CPH with the DH of 20% had the highest effect against MDA-231 and T47D cancer cell lines with growth inhibition of 78.2 and 66.2%, which were 6.5 and 6 times higher activities compared to the CPI, respectively. The amino acid profile of CPH indicated that glutamine (15.7%) and asparagine (10.9%) were predominant.

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Acknowledgements

The authors would like to thank Tarbiat Modares University for the financial support and Prof. Joe M. Regenstein for the editing.

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Authors and Affiliations

  1. Department of Seafood Processing, Tarbiat Modares University, Noor, Iran

    Ali Hamzeh & Masoud Rezaei

  2. Department of Marine Biology, Tarbiat Modares University, Noor, Iran

    Saber Khodabandeh

  3. Department of Food Science, Agricultural Sciences and Natural Resources University of Sari, Sari, Iran

    Ali Motamedzadegan

  4. Department of Hematology, Tarbiat Modares University, Tehran, Iran

    Mehrdad Noruzinia

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  1. Ali Hamzeh
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  2. Masoud Rezaei
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Correspondence to Masoud Rezaei.

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Hamzeh, A., Rezaei, M., Khodabandeh, S. et al. Antiproliferative and antioxidative activities of cuttlefish (Sepia pharaonis) protein hydrolysates as affected by degree of hydrolysis. Food Measure 12, 721–727 (2018). https://doi.org/10.1007/s11694-017-9685-0

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  • DOI: https://doi.org/10.1007/s11694-017-9685-0

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