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Expression of angiotensin II and its receptors in activated microglia in experimentally induced cerebral ischemia in the adult rats

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Abstract

Expression of angiotensin II (Ang II) and its receptors (AT1/AT2) is undetected in the mature microglia in normal brain. We report here that the immunoexpression of Ang II and AT1/AT2 was altered in activated microglia notably at 1 week in rats subjected to middle cerebral artery occlusion (MCAO). Immunolabeled activated microglia were widely distributed in the infarcted cerebral tissue after MCAO. By enzyme immunoassay, Ang II protein expression levels of the ischemic tissues were decreased drastically at 12 h after ischemia, then rose rapidly at 3 days and 1 week after MCAO when compared with the control. On the other hand, AT1 and AT2 receptor mRNA and protein levels were up-regulated after MCAO, peaking at 12 h, but declined thereafter. Expression of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) mRNA and protein levels was concomitantly increased. Edaravone significantly suppressed Ang II and AT1/AT2 receptor expression as well as that of TNF-α and IL-1β suggesting that microglia-derived Ang II can act through an autocrine manner via its receptor that may be linked partly to the production of proinflammatory cytokines. We conclude that neuroinflammation in MCAO may be attenuated by Edaravone which acts through suppression of expression of Ang II and its receptors and proinflammatory cytokines in activated microglia.

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Acknowledgments

This study is supported by the National Natural Science Research Grants of China No. 30900778 (J J Li) and 31260254 (CY Wu).

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Authors and Affiliations

  1. Department of Histology and Embryology, Faculty of Basic Medical Sciences, Kunming Medical University, 1168 West Chunrong Road, Kunming, 650500, People’s Republic of China

    Chun-Yun Wu, Hao Zha, Qing-Qing Xia, Yun Yuan, Xiao-Yan Liang, Ze-Yun Guo & Juan-Juan Li

  2. Minimally Invasive Neurosurgery, First Affiliated Hospital of Kunming Medical University, 295 Xi Chang Road, Kunming, 650032, People’s Republic of China

    Jing-Hui Li

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  1. Chun-Yun Wu
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Correspondence to Juan-Juan Li.

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Wu, CY., Zha, H., Xia, QQ. et al. Expression of angiotensin II and its receptors in activated microglia in experimentally induced cerebral ischemia in the adult rats. Mol Cell Biochem 382, 47–58 (2013). https://doi.org/10.1007/s11010-013-1717-4

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  • DOI: https://doi.org/10.1007/s11010-013-1717-4

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