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Cytogenetic mapping of immunoglobulin heavy chain genes in Antarctic fish

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Abstract

The chromosomal location of the IgH locus has been analyzed in several bony fish of the Antarctic perciform group Notothenioidei. Two IgH probes were prepared from the species Trematomus bernacchii (family Nototheniidae, tribe Trematominae) and mapped onto the chromosomes of ten species belonging to the same genus (Trematomus) and in two outgroups, through one-color and two-color FISH. A single location of the IgH locus was found in the majority of the species examined, including the outgroups, whereas in four of them the IgH genes splited to two chromosomal loci. RT-PCR experiments revealed the presence of three allelic sequences in T. newnesi, a species in which the IgH genes were organized in two chromosomal loci. Possible pathways leading to IgH genes duplication during the diversification of trematomine fishes were inferred from the analysis of the FISH patterns in a phylogenetic context. The present work provides the first comprehensive picture of IgH genes organization at chromosomal level in a bony fish group.

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Acknowledgments

This research was financially supported by the Italian National Programme for Antarctic Research (PNRA). The cytogenetic work has been realized thanks to a grant from the European Community under COLPARSYST program. Additional support was provided by the Polar French Institute Paul Emile Victor (IPEV) and by the Museum National d’Histoire Naturelle (MNHN), Paris, France. The ICEFISH Cruise was supported by National Science Foundation grant OPP-0132032. The study is in the framework of the Scientific Research Program EBA (Evolution and Biodiversity in Antarctica). Drs E. Cocca, D. de Pascale, G. di Prisco (Institute of Protein Biochemistry, Naples), and A. Rizzello (University of Lecce), kindly provided us with tissue samples for molecular analyses.

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Authors and Affiliations

  1. Department of Biology, University of Genova, Viale Benedetto XV 5, 16132, Genova, Italy

    Eva Pisano, Federico Mazzei & Laura Ghigliotti

  2. Institute of Protein Biochemistry, CNR, via Pietro Castellino 111, 80131, Napoli, Italy

    Maria Rosaria Coscia & Umberto Oreste

  3. Département Systématique et Evolution, CNRS UMR 7138, Museum National d’Histoire Naturelle, 43 rue Cuvier, 75005, Paris, France

    Jean-Pierre Coutanceau & Catherine Ozouf-Costaz

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  1. Eva Pisano
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  2. Maria Rosaria Coscia
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  3. Federico Mazzei
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  4. Laura Ghigliotti
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  5. Jean-Pierre Coutanceau
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  6. Catherine Ozouf-Costaz
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  7. Umberto Oreste
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Correspondence to Eva Pisano.

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Pisano, E., Coscia, M.R., Mazzei, F. et al. Cytogenetic mapping of immunoglobulin heavy chain genes in Antarctic fish. Genetica 130, 9–17 (2007). https://doi.org/10.1007/s10709-006-0015-4

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