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Molecular characterization and quantification of the follicle-stimulating hormone receptor in turbot (Scophthalmus maximus)

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Abstract

Molecular cloning, characterization, and functional analysis of follicle-stimulating hormone receptor (FSHR) in female turbot (Scophthalmus maximus) were evaluated. Results showed that the full-length FSHR cDNA was 3824 bp long and contained a 2202 bp open reading frame that encoded a mature protein of 733 amino acids (aa) and a signal peptide of 18 aa. Multiple sequence analyses showed that turbot FSHR has high homology with the corresponding genes of other teleosts and significant homology with that of Hippoglossus hippoglossus. Turbot FSHR has the typical structural architecture of glycoprotein hormone receptors consisting of a large N-terminal extracellular domain, seven transmembrane domains and short C-terminal intracellular domain. FSHR mRNA was found to be abundant in the ovaries, but deficient in eyes, intestine, brain, muscle, gills, spleen, stomach, heart and kidney. Furthermore, FSHR mRNA was found to increase gradually from pre-vitellogenesis to migratory nucleus stages, with the highest values observed during the late vitellogenesis stage of the reproductive cycle. However, FSHR mRNA was found to decrease dramatically during the atresia stage. Meanwhile, functional analysis with HEK293T cells continual expressing FSHR demonstrated that FSHR was specifically stimulated by ovine FSH, but not ovine LH. These results indicate that turbot FSHR is mainly involved in the stimulation of vitellogenesis, regulation of oocyte maturation as well as promotion of ovarian development via specific ligand binding. These findings open doors to further investigation of physiological functions of FSHR, which will be valuable for fish reproduction and broodstock management.

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Acknowledgments

This study was supported by China Agriculture Research System (CRAS-50), National Natural Science Foundation of China (31302205, 31402315 and 31402284), Natural Science Foundation of Shandong Province (ZR2012CQ024 and BS2013SW004) and the China Postdoctoral Science Foundation (2012M511559 and 2013T60690). We thank Chunren Gao and Xinfu Liu (Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences) for help in the experiment.

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    1. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, No. 106 Nanjing Road, Qingdao, 266071, People’s Republic of China

      Yudong Jia, Ai Sun, Zhen Meng, Baoliang Liu & Jilin Lei

    2. Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology, Qingdao, 266071, People’s Republic of China

      Yudong Jia, Ai Sun, Zhen Meng, Baoliang Liu & Jilin Lei

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    1. Yudong Jia
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    Correspondence to Jilin Lei.

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    Yudong Jia and Ai Sun have equal contribution to this work.

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    Jia, Y., Sun, A., Meng, Z. et al. Molecular characterization and quantification of the follicle-stimulating hormone receptor in turbot (Scophthalmus maximus). Fish Physiol Biochem 42, 179–191 (2016). https://doi.org/10.1007/s10695-015-0128-8

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