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Molecular cloning, characterization, and expression analysis of luteinizing hormone receptor gene in turbot (Scophthalmus maximus)

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Abstract

The luteinizing hormone receptor (LHR) plays a crucial role in female reproduction. In the present study, full-length sequence coding for the LHR was obtained from female turbot (Scophthalmus maximus) by homology cloning and a strategy based on rapid amplification of cDNA end-polymerase chain reaction. The full-length LHR cDNA was 3,184 bp long and contained a 2,058-bp open reading frame which encoded a protein of 685 amino acids. Multiple sequence alignments of the turbot LHR manifested high homologies with the corresponding sequences of available teleosts and representative vertebrates, and significant homology with that of Hippoglossus hippoglossus. In addition, the turbot LHR showed typical characteristics of glycoprotein receptors, including a long N-terminal extracellular domain, seven transmembrane domains, and a short C-terminal intracellular domain. LHR mRNA was abundant in the ovary, but was deficient in extra-ovarian tissues. Furthermore, LHR mRNA gradually developed from previtellogenesis to migratory nucleus stage, with the highest values observed in migratory nucleus stage during reproductive cycle. However, LHR mRNA sharply decreased in atresia stage. These results suggested that LHR is a typical G protein-coupled receptor that is involved in the promotion of turbot ovarian development and may be related to the final maturation and ovulation of oocyte. These findings contribute to the understanding of the potential roles of LHR in controlling the fish reproductive cycle.

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Acknowledgments

This study was supported by China Agriculture Research System (CRAS-50), National Natural Science Foundation of China (31302205), Natural Science Foundation of Shandong Province (ZR2012CQ024 and BS2013SW004), and the China Postdoctoral Science Foundation (No. 2012M511559 and 2013T60690). We thank Xinfu Liu and Chunren Gao (Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences) for help in the experiment.

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Authors and Affiliations

  1. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, No. 106 Nanjing Road, Qingdao, 266071, People’s Republic of China

    Yudong Jia, Zhen Meng, Peng Hu & Jilin Lei

  2. Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology, Qingdao, 266071, People’s Republic of China

    Yudong Jia, Zhen Meng, Peng Hu & Jilin Lei

  3. School of Animal Science and Technology, Inner Mongolia University for the Nationalities, Tongliao, 028042, People’s Republic of China

    Huaxin Niu

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  1. Yudong Jia
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  2. Zhen Meng
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  3. Huaxin Niu
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Correspondence to Jilin Lei.

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Yudong Jia and Zhen Meng have contributed equally to this work.

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Jia, Y., Meng, Z., Niu, H. et al. Molecular cloning, characterization, and expression analysis of luteinizing hormone receptor gene in turbot (Scophthalmus maximus). Fish Physiol Biochem 40, 1639–1650 (2014). https://doi.org/10.1007/s10695-014-9954-3

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