Abstract
Breeding for resistance against Bacterial blight (BB), a destructive disease in rice, is a major challenge because of the rich diversity of the pathogen, Xanthomonas oryzae pv. oryzae. Three resistance genes i.e. xa5, xa13 and Xa21 were transferred from IRBB 60 through marker-assisted backcross breeding to supplement the Xa4 gene present in Tapaswini, an elite cultivar having a wide coverage. The precise transfer was aided through effective foreground selection using STS markers RG 556, RG 136 and pTA248 linked to the three target genes, respectively. In the screening assays, the gene pyramids having four genes exhibited higher level of resistance against the disease. Background selection based on morphological and grain quality traits and SSR markers led to the recovery of 85–96 % of the recurrent parent genome in the gene pyramids. Multi location evaluation conducted over a two year period at several hotspot locations of bacterial blight in India has resulted in identification and release of CRMAS 2622-7-6 (IT-6), a promising gene pyramid as improved Tapaswini. The high levels of resistance exhibited in the improved genotype without any yield penalty and its similarity to the recurrent parent provides a successful demonstration of the potential of MAS in pyramiding genes as well as the recovery of the parental genome with high accuracy.
Similar content being viewed by others
References
Annual Report 2011–12 Central Rice Research Institute Cuttack, p 21
Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA mini preparation: version II. Plant Mol Biol Rep 1:19–21
Directorate of Rice Research (2009) Progress report, 2008, varietal improvement, vol 1. AICRIP (ICAR) Directorate of Rice Research, Hyderabad
Directorate of Rice Research (2010) Progress report, 2009, Varietal Improvement, Vol 1. AICRIP (ICAR) Directorate of Rice Research, Hyderabad
Goel RK, Kaur L, Saini RG (1998) Effectiveness of different Xa genes against Xanthomonas oryzae pv. oryzae population causing bacterial blight of rice in Punjab (India). Rice Genet Newsl 15:131–133
Huang N, Angeles ER, Domingo J, Magpantay G, Singh S, Zhang G, Kumaravadivel N, Bennett J, Khush GS (1997) Pyramiding of bacterial blight resistance genes in rice: marker-assisted selection using RFLP and PCR. Theor Appl Genet 95:313–320
Joseph M, Gopalakrishnan S, Sharma RK, Singh VP, Singh AK, Singh NK, Mohapatra T (2004) Combining bacterial blight resistance and basmati quality characteristics by phenotypic and molecular marker assisted selection in rice. Mol Breed 13:377–387
Kauffman HE, Reddy APK, Hsien SPY, Merca SD (1973) An improved technique for evaluating resistance of rice varieties to Xanthomonas oryzae. Plant Dis Report 57:537–541
Khush GS, Mackill DJ, Sidhu GS (1989) Breeding rice for resistance to bacterial blight. In: Bacterial blight of rice. Proc Int workshop on bacterial blight of rice, International Rice Research Institute, Manila, 207−217 March 1988
Ma BJ, Wang WM, Zhao B, Zhou YL, Zhu LH, Zhai WX (1999) Study on the PCR marker for the rice bacterial blight resistance gene Xa4. Hereditas 2:9
Mew TW, VeraCruz CM, Medalla ES (1992) Changes in race frequency of Xanthomonas oryzae pv. oryzae in response to the planting of rice cultivars in the Philippines. Plant Dis 76:1029–1032
Mundt CC (1990) Probability of mutation to multiple virulence and durability of resistance gene pyramids. Phytophathology 80:221–223
Narayanan NN, Baisakh N, Oliva NP, VeraCruz CV, Gnanamanickam SS, Datta K, Datta SK (2004) Molecular breeding: marker-assisted selection combined with biolistic transformation for blast and bacterial blight resistance in indica rice (cv. CO 39). Mol Breed 14:61–67
OU SH (1972) Rice diseases. Commonwealth Mycological Institute, Surrey (revised edition, 1985)
Perez ML, Redona DE, Mendioro SM, Verna Cruz MC, Leung H (2008) Introgression of Xa4, Xa7 and Xa21 for resistance to bacterial blight in thermo sensitive genetic male sterile rice (Oryza sativa L.) for the development of two line hybrids. Euphytica 164:627–636
Podishetty N, Kalidindi S, Laha GS, Kommoju S, Mishra B, Viraktamath B, Yadla H, Reddy C, Balachandran S, Ram T (2011) Identification and fine-mapping of Xa33, a novel gene for resistance to Xanthomonas oryzae pv oryzae. Phytopathology 102:222–228
Rohlf FJ (1998) Numerical taxonomy and multivariate analysis system-NTSYS-pc-ver. 2.02f. Exeter Software, New York
Ronald PC, Albato B, Tabien R, Abenes MLP, Wu KS, McCouch SR, Tanksley SD (1992) Genetic and physical analysis of the rice bacterial blight disease resistance locus Xa21. Mol Gen Genet 236:113–120
Sanchez AC, Brar DS, Huang N, Li Z, Khush GS (2000) Sequence tagged site marker-assisted selection for three bacterial blight resistance genes in rice. Crop Sci 40:792–797
Shin MS, Oh MK, Noh TH, Kim KY, Kim BK, Ko JK, Lee JK (2004) Development of japonica rice “Iksan478” carrying Xa7 resistant gene to bacterial blight. In: Proceedings of the 4th international crop science congress, Brisbane
Singh GP, Srivastava MK, Singh RV, Singh RM (1997) Variation and qualitative loses caused by bacterial blight in different rice varieties. Ind. Phytopathol 30:180–185
Singh S, Sidhu JS, Huang N, Vikal Y, Li Z, Brar DS, Dhaliwal HS, Khush GS (2001) Pyramiding three bacterial blight resistance genes (xa-5, xa-13 and Xa-21) using marker-assisted selection into indica rice cultivar PR 106. Theor Appl Genet 102:1011–1015
Singh AK, Gopalakrishnan S, Singh VP, Prabhu KV, Mohapatra T, Singh NK, Sharma TR, Nagarajan M, Vinod KK, Singh D, Singh UD, Chander S, Atwal SS, Seth R, Singh VK, Ellur RK, Singh A, Anand D, Khanna A, Yadav S, Goel N, Singh A, Shikari AB, Singh A, Marathi B (2011) Marker assisted selection: a paradigm shift in Basmati breeding. Ind J Gen Pl Breed 71, Special Issue:1–9
Sundaram RM, Vishnupriya RM, Biradar SK, Laha GS, Ashok Reddy G, Shobha Rani N, Sarma NP, Sonti RV (2008) Marker assisted introgression of bacterial blight resistance in Samba Mahsuri, an elite indica rice variety. Euphytica 160:411–422
Sundaram RM, Vishnupriya RM, Laha GS, Shobha Rani N, Srinivas Rao P, Balachandaran SM, Ashok Reddy G, Sarma NP, Sonti RV (2009) Introduction of bacterial blight resistance into Triguna, a high yielding, mid-early duration rice variety. Biotechnol J 4:400–407
Tanksley SD, Young ND, Paterson AH, Bonierbale MW (1989) RFLP mapping in plant breeding: new tools for an old science. Biotechnology 7:257–264
Van Berloo R (1999) GGT: software for display of graphical genotypes. J Hered 90:328–329
Widawsky DA, O’Toole JC (1990) Prioritizing the rice research agenda for eastern India. Rockefeller Foundation, NewYork, p 86
Yoshimura S, Yoshimura A, Iwata N, McCouch SR, Abenes A, Baraoidan MR, Mew TW, Nelson RJ (1995) Tagging and combining bacterial blight resistance genes in rice using RAPD and RFLP markers. Mol Breed 1:375–387
Young ND, Tanksley SD (1989) RFLP analysis of the size of chromosomal segments retained around the Tm-2 locus of tomato during backcross breeding. Theor Appl Genet 77:353–359
Zhang G, Angeles ER, Abenes MLP, Khush GS, Huang N (1996) RAPD and RFLP mapping for bacterial blight resistance gene Xa13 in rice. Theor Appl Genet 93:65–70
Zheng K, Huang N, Bennett J, Khush G.S (1995) PCR-based marker-assisted selection in rice breeding. IRRI Discussion Paper, International Rice Research Institute, Manila, pp 12, 24
Acknowledgments
The authors are highly grateful to the Director, Central Rice Research Institute, Cuttack for providing all the necessary facilities.
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Dokku, P., Das, K.M. & Rao, G.J.N. Pyramiding of four resistance genes of bacterial blight in Tapaswini, an elite rice cultivar, through marker-assisted selection. Euphytica 192, 87–96 (2013). https://doi.org/10.1007/s10681-013-0878-2
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10681-013-0878-2