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Evaluation of a liquid formulation of Pseudomonas fluorescens against Fusarium oxysporum f. sp. cubense and Helicotylenchus multicinctus in banana plantation

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Abstract

Plant growth promoting rhizobacteria (PGPR) are eco-friendly alternatives to chemical fungicides to manage plant diseases. We evaluated the efficacy of a Pseudomonas fluorescens formulation against Fusarium oxysporum f. sp. cubense and Helicotylenchus multicinctus at multiple banana plantations. Three field trials were conducted to assess the wilt incidence and the populations of nematode and bacteria in the soil treated with a liquid formulation of P. fluorescens at 2.0, 3.0 and 4.0 l ha−1 using drip irrigation system at 60, 120, 180 and 240 days after planting. The results showed that the treatment at 4.0 l ha−1 reduced the wilt incidence by 60 %. It also reduced the overall population of H. multicinctus by 41.3–89.0 % in the treated fields. The presence of P. fluorescens in the treated soil was 5.6 × 106 cfu g−1 of soil at the time of harvest. The treatment of biocontrol agent P. fluorescens also resulted in an overall yield increase in banana production by 36.6–46.5 % compared to the control.

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References

  • Ajit NS, Verma R, Shanmugam V (2006) Extracellular chitinases of fluorescent pseudomonads antifungal to Fusarium oxysporum f. sp. dianthi causing carnation wilt. Curr Microbiol 52:310–316

    Article  CAS  PubMed  Google Scholar 

  • Aurore G, Parfait B, Fahrasmane L (2009) Bananas, raw materials for making processed food products. Trends Food Sci Technol 20:78–91

    Article  CAS  Google Scholar 

  • Bujulu J, Uronu B, Cumming CNC (1996) The control of banana weevils and parasitic nematodes in Tanzania. East Afr Agric For J 49:1–13

    Google Scholar 

  • Carlier J, De Waele D, Escalante JV (2003) Global evaluation of Musa germplasm for resistance to Fusarium wilt, Mycosphaerella leaf spot diseases and nematodes. In: Vegine A, Pig C (eds) INIBAP Technical guidelines 7—performance evaluation. INIBAP, Montpellier, France, pp 21–24

    Google Scholar 

  • Chavan BP, Kadam JR, Saindane YS (2008) Bioefficacy of liquid formulation of Verticillium lecanii against aphid (Aphis gossypii). Int J Plant Prot 1:69–72

    Google Scholar 

  • Cobb NA (1918) Estimating the nematode population of soil.  Agricultural Technical Circular No.1, USDA, USA

  • de Boer M, Bom P, Kindt F, Keurentjes JJB, van der Sluis I, van Loon LC, Bakker PAHM (2003) Control of Fusarium wilt of radish by combining Pseudomonas putida strains that have different disease-suppressive mechanisms. Phytopathology 93:626–632

    Article  PubMed  Google Scholar 

  • Gomez KA, Gomez AA (1984) Statistical procedure for agricultural research, 2nd edn. Wiley, New York, USA

    Google Scholar 

  • Hegde SV (2002) Liquid biofertilizers in Indian agriculture. Biofertilizer Newsl 12:17–22

    Google Scholar 

  • Jonathan EI, Cannayane I, Samiyappan R (2004) Field application of biocontrol agents for the management of spiral nematode, Helicotylenchus multicinctus, in banana. Nematol Medit 32:169–173

    Google Scholar 

  • King EO, Ward MK, Raney DE (1954) Two simple media for the demonstration of pyocyanin and fluorescein. J Lab Clin Med 44:301–307

    CAS  PubMed  Google Scholar 

  • Manikandan R, Saravanakumar D, Rajendran L, Raguchander T, Samiyappan R (2010) Standardization of liquid formulation of Pseudomonas fluorescens Pf1 for its efficacy against Fusarium wilt of tomato. Biol Control 54:83–89

    Article  Google Scholar 

  • McKinney’s HH (1923) A new system of grading plant diseases. J Agric Res 26:195–218

    Google Scholar 

  • Nayaka C, Udaya Shankar S, Reddy AC, Niranjana MS, Prakash SR, Shetty HS, Mortensen CN (2009) Control of Fusarium verticillioides, cause of ear rot of maize, by Pseudomonas fluorescens. Pest Manag Sci 65:769–775

    Article  CAS  PubMed  Google Scholar 

  • Oostendorp M, Sikora RA (1989) Seed treatment with antagonistic rhizobacteria for the suppression of Heterodera schachtii early root infection of sugar beet. Rev Nematol 12:77–83

    Google Scholar 

  • Ploetz RC (2006) Fusarium wilt of banana is caused by several pathogens referred to as Fusarium oxysporum f. sp. cubense. Phytopathology 96:653–656

    Article  PubMed  Google Scholar 

  • Ploetz RC, Haynes JL, Vazquez A (1999) Responses of new banana accessions in South Florida to Panama disease. Crop Prot 18:445–449

    Article  Google Scholar 

  • Punja ZK, Zhang YY (1993) Plant chitinase and their role in resistance to fungal diseases. J Nematol 25:526–540

    CAS  PubMed Central  PubMed  Google Scholar 

  • Raguchander T, Jayashree K, Samiyappan R (1997) Management of Fusarium wilt of banana using antagonistic micro-organisms. J Biol Control 11:101–105

    Google Scholar 

  • Rajendran G, Sivakumar CV (1996) Pathogenicity of the spiral nematode Helicotylenchus multicinctus (Cobb, 1893) Golden, 1956 and effect on yield and nutrient status of banana. Pest Manag Hortic Ecosyst 2:23–27

    Google Scholar 

  • Ramamoorthy V, Raguchander T, Samiyappan R (2002) Induction of defense-related proteins in tomato roots treated with Pseudomonas fluorescens Pf1 and Fusarium oxysporum f. sp. lycopersici. Plant Soil 239:55–68

    Article  CAS  Google Scholar 

  • Saikia R, Singh T, Kumar R, Srivastava J, Srivastava AK, Singh K, Arora DK (2003) Role of salicylic acid in systemic resistance induced by Pseudomonas fluorescens against Fusarium oxysporum f. sp. ciceri in chickpea. Microbiol Res 158:203–213

    Article  CAS  PubMed  Google Scholar 

  • Saravanakumar D, Lavanya N, Muthumeena K, Raguchander T, Samiyappan R (2009) Fluorescent pseudomonad mixtures mediate disease resistance in rice plants against sheath rot (Sarocladium oryzae) disease. BioControl 54:273–286

    Article  Google Scholar 

  • Saravanan T, Muthusamy M, Marimuthu T (2004) Effect of Pseudomonas fluorescens on Fusarium wilt pathogen in banana rhizosphere. J Biol Sci 4:192–198

    Article  Google Scholar 

  • Schindler AF (1961) A simple substitute for a Baermann funnel. Plant Dis Rep 45:747–748

    Google Scholar 

  • Seenivasan N (2011) Efficacy of Pseudomonas fluorescens and Paecilomyces lilacinus against Meloidogyne graminicola infesting rice under system of rice intensification. Arch Phytopathol Plant Prot 44:1467–1482

    Article  Google Scholar 

  • Seenivasan N, Devrajan K (2008) Management of root-knot nematode on medicinal coleus, Coleus forskohlii (wild.) briq. by commercial biocontrol formulations. Nematol Medit 36:61–67

    Google Scholar 

  • Seenivasan N, Lakshmanan PL (2001) Effect of culture filtrates of Pseudomonas fluorescens on rice root nematode, Hirschmanniella gracilis. Pestology 25:11–12

    Google Scholar 

  • Seenivasan N, Murugan VT (2011a) Bio-management of reniform nematode-Fusarium wilt disease complex in cotton. J Plant Prot Environ 8:90–96

    Google Scholar 

  • Seenivasan N, Murugan VT (2011b) Optimization of delivery methods for Pseudomonas fluorescens in management of rice root nematode, Hirschmanniella gracilis. Ann Plant Prot Sci 19:188–192

    Google Scholar 

  • Seenivasan N, Poornima K (2010) Bio-management of root-knot nematode, Meloidogyne incognita (Kofoid and White) Chitwood in Jasmine (Jasminum sambac L.). Pest Manag Hortic Ecosyst 16:34–40

    Google Scholar 

  • Seenivasan N, David PMM, Vivekanandan P, Samiyappan R (2012) Biological control of rice root-knot nematode, Meloidogyne graminicola through mixture of Pseudomonas fluorescens strains. Biocontrol Sci Tech 22:611–632

    Article  Google Scholar 

  • Senthil R, Selvaraj S, Anand T, Raguchander T, Samiyappan R (2011) Efficacy of liquid Pseudomonas fluorescens (Pf1) against sugarcane red rot, caused by Colletotrichum falcatum under field condition. Int Sugar J 113:888–893

    CAS  Google Scholar 

  • Sikora RA, Schlosser E (1973) Nematodes and fungi associated with root systems of bananas in a state of decline in Lebanon. Plant Dis Rep 57:615–618

    Google Scholar 

  • Sivamani E, Gnanamanickam SS (1988) Biological control of Fusarium oxysporum f. sp cubense in banana by inoculation with Pseudomonas fluorescens. Plant Soil 107:3–9

    Article  Google Scholar 

  • Stover RH (1990) Fusarium wilt of banana: some history and current status of the disease. In: Ploetz RC (ed) Fusarium wilt of banana. APS Press, St. Paul, USA, pp 1–7

  • Vendan RT, Thangaraju M (2006) Development and standardization of liquid formulation for Azospirillum bioinoculant. Indian J Microbiol 46:379–387

    CAS  Google Scholar 

  • Vidhyasekaran P, Muthamilan M (1995) Development of formulation of Pseudomonas fluorescens for control of chickpea wilt. Plant Dis 79:782–786

    Article  Google Scholar 

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Acknowledgments

We thank the anonymous reviewers for providing valuable comments on this manuscript. The authors also extend their thanks to Dr. P. M. M. David, Professor (Entomology), Tamil Nadu Agricultural University, Dr. B. Augustine Jerard, Senior Scientist (Horticulture), Central Plantation Crops Research Institute, Kerala and Dr. Ing. Marek Renco, Slovak Academy of Sciences, Institute of Parasitology, Kosice, Slovak Republic for their valuable suggestions. We thank Prof. Dr. Muraleedharan G. Nair, Professor and Senior Associate to the Dean, Michigan State University, East Lansing, USA for English correction of the manuscript. The authors would like to thank the Ministry of Agriculture, Government of India for financial assistantship (NADP Scheme).

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Authors and Affiliations

  1. Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore, 641003, Tamil Nadu, India

    S. Selvaraj, P. Ganeshamoorthi, T. Anand & T. Raguchander

  2. Department of Nematology, Tamil Nadu Agricultural University, Coimbatore, 641003, Tamil Nadu, India

    N. Seenivasan

  3. Centre for Plant and Molecular Biology, Tamil Nadu Agricultural University, Coimbatore, 641003, Tamil Nadu, India

    R. Samiyappan

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  1. S. Selvaraj
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  2. P. Ganeshamoorthi
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  3. T. Anand
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  4. T. Raguchander
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  5. N. Seenivasan
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  6. R. Samiyappan
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Correspondence to N. Seenivasan.

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Handling Editor: Jesus Mercado Blanco.

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Selvaraj, S., Ganeshamoorthi, P., Anand, T. et al. Evaluation of a liquid formulation of Pseudomonas fluorescens against Fusarium oxysporum f. sp. cubense and Helicotylenchus multicinctus in banana plantation. BioControl 59, 345–355 (2014). https://doi.org/10.1007/s10526-014-9569-8

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