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Fluorescent pseudomonad mixtures mediate disease resistance in rice plants against sheath rot (Sarocladium oryzae) disease

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Abstract

Plant growth-promoting rhizobacterial (PGPR) strains were isolated from different agro-ecosystems of Tamil Nadu, India, and were tested for their efficacy against the sheath rot pathogen Sarocladium oryzae under in vitro, glasshouse and field conditions. Vigour and a relative performance index (RPI) were used to assay the growth promotion and antagonistic activity of Pseudomonas strains against S. oryzae under in vitro conditions. The results revealed the significant performance by strains Pf1, TDK1 and PY15 compared to other strains. Further, the combination of Pseudomonas strains Pf1, TDK1 and PY15 was more effective in reducing sheath rot disease in rice plants compared to individual strains under glasshouse and field conditions. Quantitative and native polyacrylamide gel electrophoresis (PAGE) analysis of peroxidase (PO), polyphenol oxidase (PPO) and chitinase activity in rice plants showed an increased accumulation of defence enzymes in the treatment with a combination of Pf1, TDK1 and PY15 compared to the treatment with individual strains and untreated controls. The present study revealed the probable influence of antagonism, plant growth promotion and induced systemic resistance (ISR) by the mixture of Pseudomonas bioformulations in enhancing the disease resistance in rice plants against sheath rot disease.

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Acknowledgement

The authors are grateful to the Department of Biotechnology (DBT), Ministry of Science and Technology, Government of India, for their financial assistance in carrying out this work.

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Authors and Affiliations

  1. Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore, 641 003, Tamil Nadu, India

    Duraisamy Saravanakumar, Nallathambi Lavanya, Kannappan Muthumeena, Thiruvengadam Raguchander & Ramasamy Samiyappan

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  1. Duraisamy Saravanakumar
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  2. Nallathambi Lavanya
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  3. Kannappan Muthumeena
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  4. Thiruvengadam Raguchander
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  5. Ramasamy Samiyappan
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Correspondence to Duraisamy Saravanakumar.

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Handling editor: Monica Höfte.

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Saravanakumar, D., Lavanya, N., Muthumeena, K. et al. Fluorescent pseudomonad mixtures mediate disease resistance in rice plants against sheath rot (Sarocladium oryzae) disease. BioControl 54, 273–286 (2009). https://doi.org/10.1007/s10526-008-9166-9

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