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Flavobacterium arsenitoxidans sp. nov., an arsenite-oxidizing bacterium from Thai soil

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Abstract

An arsenite-oxidizing bacterium, strain S2-3HT, was isolated from arsenic-contaminated soil sample collected from Dantchaeng district, Suphanburi province, Thailand and was characterized based on polyphasic taxonomic study. The strain was observed to be a Gram-stain negative, aerobic, yellow pigmented, non-spore forming and rod-shaped bacterium. Major menaquinone was MK-6. Iso-C15:0, iso-C15:0 3OH, C16:1 ω7c/C16:1 ω6c, C16:0, iso-C17:0 3OH, and C16:0 3OH were the predominant cellular fatty acids. The polar lipid profile consisted of phosphatidylethanolamine, unidentified phospholipids and unidentified aminophospholipids. The DNA G+C content was 37.0 mol%. Phylogenetic analysis using 16S rRNA sequence showed that strain S2-3HT is affiliated to the genus Flavobacterium, and is closely related to F. defluvii KCTC 12612T (97.0 %) and F. johnsoniae NBRC 14942T (97.0 %). The strain S2-3HT could be clearly distinguished from the related Flavobacterium species by its physiological and biochemical characteristics as well as its phylogenetic position and DNA–DNA relatedness. Therefore, the strain represents a novel species of the genus Flavobacterium, for which the name Flavobacterium arsenitoxidans sp. nov. (type strain S2-3HT = KCTC 22507T = NBRC 109607T = PCU 331T = TISTR 2238T) is proposed.

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Acknowledgments

This work was supported by UNU& GIST research grant (2008) and a grant from KRIBB Research Initiative Program are gratefully acknowledged. The authors thank Mr. Paichayon Charoenchaisri, Bureau of Environmental Management, Department of Primary Industries and Mines, Ministry of Industry for collecting the soil sample.

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Authors and Affiliations

  1. Faculty of Animal Sciences and Agricultural Technology, Silpakorn University, Phetchaburi IT Campus, Phetchaburi, 76120, Thailand

    Saowapar Khianngam

  2. Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand

    Ancharida Akaracharanya

  3. Korean Collection for Type Cultures, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, Republic of Korea

    Jung-Sook Lee & Keun Chul Lee

  4. Department of Environmental Science and Engineering, Gwangju Institute of Science and Technology, Gwangju, 500-712, Republic of Korea

    Kyoung-Woong Kim

  5. Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, 10330, Thailand

    Somboon Tanasupawat

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  1. Saowapar Khianngam
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  2. Ancharida Akaracharanya
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  3. Jung-Sook Lee
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  4. Keun Chul Lee
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  5. Kyoung-Woong Kim
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  6. Somboon Tanasupawat
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Correspondence to Somboon Tanasupawat.

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Khianngam, S., Akaracharanya, A., Lee, JS. et al. Flavobacterium arsenitoxidans sp. nov., an arsenite-oxidizing bacterium from Thai soil. Antonie van Leeuwenhoek 106, 1239–1246 (2014). https://doi.org/10.1007/s10482-014-0294-1

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  • DOI: https://doi.org/10.1007/s10482-014-0294-1

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