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Use of Cupriavidus basilensis-aided bioabatement to enhance fermentation of acid-pretreated biomass hydrolysates by Clostridium beijerinckii

  • Bioenergy/Biofuels/Biochemicals
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Lignocellulose-derived microbial inhibitors (LDMICs) prevent efficient fermentation of Miscanthus giganteus (MG) hydrolysates to fuels and chemicals. To address this problem, we explored detoxification of pretreated MG biomass by Cupriavidus basilensis ATCC®BAA-699 prior to enzymatic saccharification. We document three key findings from our test of this strategy to alleviate LDMIC-mediated toxicity on Clostridium beijerinckii NCIMB 8052 during fermentation of MG hydrolysates. First, we demonstrate that growth of C. basilensis is possible on furfural, 5-hydroxymethyfurfural, cinnamaldehyde, 4-hydroxybenzaldehyde, syringaldehyde, vanillin, and ferulic, p-coumaric, syringic and vanillic acid, as sole carbon sources. Second, we report that C. basilensis detoxified and metabolized ~98 % LDMICs present in dilute acid-pretreated MG hydrolysates. Last, this bioabatement resulted in significant payoffs during acetone-butanol-ethanol (ABE) fermentation by C. beijerinckii: 70, 50 and 73 % improvement in ABE concentration, yield and productivity, respectively. Together, our results show that biological detoxification of acid-pretreated MG hydrolysates prior to fermentation is feasible and beneficial.

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Acknowledgments

Salaries and research support were provided in part by State funds appropriated to the Ohio Plant Biotechnology Consortium by The Ohio State University, Ohio Agricultural Research and Development Center (OARDC), Peloton Technologies LLC, and the Hatch grant (Project No. OHO01333).

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Authors and Affiliations

  1. Department of Animal Sciences, Ohio State Agricultural Research and Development Center (OARDC), The Ohio State University, 305 Gerlaugh Hall, 1680 Madison Avenue, Wooster, OH, 44691, USA

    Chidozie Victor Agu & Thaddeus Chukwuemeka Ezeji

  2. Renewable Energy Program, Agricultural Technical Institute, The Ohio State University, 1328 Dover Road, Wooster, OH, 44691, USA

    Victor Ujor

  3. Department of Chemistry and Biochemistry, Center for RNA Biology, The Ohio State University, 484 West 12th Avenue, Columbus, OH, 43210, USA

    Venkat Gopalan

Authors
  1. Chidozie Victor Agu
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  2. Victor Ujor
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  3. Venkat Gopalan
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  4. Thaddeus Chukwuemeka Ezeji
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Corresponding author

Correspondence to Thaddeus Chukwuemeka Ezeji.

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Agu, C.V., Ujor, V., Gopalan, V. et al. Use of Cupriavidus basilensis-aided bioabatement to enhance fermentation of acid-pretreated biomass hydrolysates by Clostridium beijerinckii . J Ind Microbiol Biotechnol 43, 1215–1226 (2016). https://doi.org/10.1007/s10295-016-1798-7

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  • DOI: https://doi.org/10.1007/s10295-016-1798-7

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