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Inheritance and Variation of Genomic DNA Methylation in Diploid and Triploid Pacific Oyster (Crassostrea gigas)

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Abstract

DNA methylation is an important epigenetic mechanism that could be responsive to environmental changes indicating a potential role in natural selection and adaption. In order to evaluate an evolutionary role of DNA methylation, it is essential to first gain a better insight into inheritability. To address this question, this study investigated DNA methylation variation from parents to offspring in the Pacific oyster Crassostrea gigas using fluorescent-labeled methylation-sensitive amplified polymorphism (F-MSAP) analysis. Most of parental methylated loci were stably transmitted to offspring segregating following Medelian expectation. However, methylated loci deviated more often than non-methylated loci and offspring showed a few de novo methylated loci indicating DNA methylation changes from parents to offspring. Interestingly, some male-specific methylated loci were found in this study which might help to explore sex determination in oyster. Despite environmental stimuli, genomic stresses such as polyploidization also can induce methylation changes. This study also compared global DNA methylation level and individual methylated loci between diploid and triploid oysters. Results showed no difference in global methylation state but a few ploidy-specific loci were detected. DNA methylation variation during polyploidization was less than autonomous methylation variation from parents to offspring.

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Acknowledgments

This study was supported by grants from the National High Technology Research and Development Program (2012AA10A405-6), National Natural Science Foundation of China (31372524), and Special Fund for Independent Innovation of Shandong Province (2013CX80202).

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  1. The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, 266003, China

    Qun Jiang, Qi Li, Hong Yu & Lingfeng Kong

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  1. Qun Jiang
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Correspondence to Qi Li.

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Jiang, Q., Li, Q., Yu, H. et al. Inheritance and Variation of Genomic DNA Methylation in Diploid and Triploid Pacific Oyster (Crassostrea gigas). Mar Biotechnol 18, 124–132 (2016). https://doi.org/10.1007/s10126-015-9674-4

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