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Fabrication of a copper nanoparticle/chitosan/carbon nanotube-modified glassy carbon electrode for electrochemical sensing of hydrogen peroxide and glucose

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Abstract.

A high-performance amperometric glucose biosensor was developed, based on immobilization of glucose oxidase (GOx) on a copper (Cu) nanoparticles/chitosan (CHIT)/carbon nanotube (CNT)-modified glassy carbon (GC) electrode. The Cu and CNT had a synergistic electrocatalytic effect toward the reduction of hydrogen peroxide in the matrix of biopolymer CHIT. The Cu/CHIT/CNT modified GC electrode could amplify the reduction current of hydrogen peroxide greatly. Besides, the Cu/CHIT/CNT modified GC electrode reduces hydrogen peroxide at a much lower applied potential and inhibit the responses of interferents. With GOx as an enzyme model, a new glucose biosensor was fabricated. The sensitivity of the sensor is due not only to the large microscopic area but also to the high efficiency of transformation of H2O2 generated by enzymatic reaction to current signal. The biosensor exhibited excellent sensitivity (the detection limit is down to 0.02 mM), fast response time (less than 4 sec), wide linear range (from 0.05 to 12 mM), and perfect selectivity.

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References

  • S F White I E Tohill J D Newman A P F Turner (1996) ArticleTitleDevelopment of a mass-producible glucose biosensor and flow-injection analysis system suitable for on-line monitoring during fermentations Anal Chim Acta 321 IssueID2–3 165 Occurrence Handle10.1016/0003-2670(95)00514-5 Occurrence Handle1:CAS:528:DyaK28XhsFOjtr4%3D

    Article  CAS  Google Scholar 

  • L A Terry S F White L J Tigwell (2005) ArticleTitleThe application of biosensors to fresh produe and the wider food industry J Agric Food Chem 53 IssueID5 1309 Occurrence Handle10.1021/jf040319t Occurrence Handle1:CAS:528:DC%2BD2MXptlSntQ%3D%3D

    Article  CAS  Google Scholar 

  • H Yang T D Chung Y T Kim C A Choi C H Jun H C Kim (2002) ArticleTitleGlucose sensor using a microfabricated electrode and electropolymerized bilayer films Biosens Bioelectron 17 251 Occurrence Handle10.1016/S0956-5663(01)00266-4 Occurrence Handle1:CAS:528:DC%2BD38XhtFyrtro%3D

    Article  CAS  Google Scholar 

  • A A Karyakin E E Karyakina L Gorton (1996) ArticleTitlePrussian Blue-based amperometric biosensors in flow-injection analysis Talanta 43 1597 Occurrence Handle10.1016/0039-9140(96)01909-1 Occurrence Handle1:CAS:528:DyaK28XlsFOitb0%3D

    Article  CAS  Google Scholar 

  • R Garjonyte A Malinauskas (2000) ArticleTitleGlucose biosensor based on glucose oxidase immobilized in electropolymerized polypyrrole and poly(o-phenylenediamine) films on a Prussian Blue-modified electrode Sens Actuators B 63 122 Occurrence Handle10.1016/S0925-4005(00)00317-8

    Article  Google Scholar 

  • D W Pan J H Chen L H Nie W Y Tao S Z Yao (2004) ArticleTitleAn amperometric glucose biosensor based on poly(o-aminophenol) and Prussian blue films at platinum electrode Anal Biochem 324 115 Occurrence Handle10.1016/j.ab.2003.09.029 Occurrence Handle1:CAS:528:DC%2BD3sXptl2qt7g%3D

    Article  CAS  Google Scholar 

  • S Iijima (1991) ArticleTitleHelical microtubules of graphitic carbon Nature 354 56 Occurrence Handle10.1038/354056a0 Occurrence Handle1:CAS:528:DyaK38Xmt1Ojtg%3D%3D

    Article  CAS  Google Scholar 

  • T W Ebbesen (1996) ArticleTitleCarbon nanotubes Phys Today 49 26 Occurrence Handle1:CAS:528:DyaK28XktlyktLY%3D

    CAS  Google Scholar 

  • Y Lin R X Wang X M Li (2005) ArticleTitleElectrochemical oxidation behavior of nitrite on a chitosan-carboxylated multiwall carbon nanotube modified electrode Electrochem Commun 7 IssueID6 597 Occurrence Handle10.1016/j.elecom.2005.04.009 Occurrence Handle1:CAS:528:DC%2BD2MXksV2hsrk%3D

    Article  CAS  Google Scholar 

  • S Hrapovic Y Liu K B Male (2004) ArticleTitleElectrochemical biosensing platforms using platinum nanoparticles and carbon nanotubes Anal Chem 76 IssueID6 1083 Occurrence Handle10.1021/ac035143t Occurrence Handle1:CAS:528:DC%2BD3sXpvVChs78%3D

    Article  CAS  Google Scholar 

  • Y C Tsai S C Li J M Chen (2005) ArticleTitleCast thin film biosensor design based on a nafion backbone, a multiwalled carbon nanotube conduit, and a glucose oxidase function Langmuir 21 IssueID8 3653 Occurrence Handle10.1021/la0470535 Occurrence Handle1:CAS:528:DC%2BD2MXitVCktL0%3D

    Article  CAS  Google Scholar 

  • M G Zhang A Smith W Gorski (2004) ArticleTitleCarbon nanotube-chitosan system for electrochemical sensing based on dehydrogenase enzymes Anal Chem 76 IssueID17 5045 Occurrence Handle10.1021/ac049519u Occurrence Handle1:CAS:528:DC%2BD2cXmtVSntLw%3D

    Article  CAS  Google Scholar 

  • S X Zhang N Wang H J Yu Y M Niu C Q Sun (2005) ArticleTitleCovalent attachment of glucose oxidase to an Au electrode modified with gold nanoparticles for use as glucose biosensor Bioelectrochemistry 67 IssueID1 15 Occurrence Handle10.1016/j.bioelechem.2004.12.002 Occurrence Handle1:CAS:528:DC%2BD2MXlsFCrtbc%3D

    Article  CAS  Google Scholar 

  • S X Zhang N Wang Y M Niu C Q Sun (2005) ArticleTitleImmobilization of glucose oxidase on gold nanoparticles modified Au electrode for the construction of biosensor Sensor and Actuators B: Chemical 109 IssueID2 367 Occurrence Handle10.1016/j.snb.2005.01.003 Occurrence Handle1:CAS:528:DC%2BD2MXmvVWntL0%3D

    Article  CAS  Google Scholar 

  • Y Ikariyama S Yamauchi T Yukiashi H Ushioda (1987) ArticleTitleOne Step fabrication of microbiosensor prepared by the codeposition of enzyme and platimum particles Anal Lett 20 1791 Occurrence Handle1:CAS:528:DyaL1cXjsVansg%3D%3D

    CAS  Google Scholar 

  • H H Zhou H Chen S L Luo J H Chen W Z Wei Y F Kuang (2005) ArticleTitleGlucose biosensor based on platinum microparticles dispersed in nano-fibrous polyaniline Biosens Bioelectron 20 1305 Occurrence Handle10.1016/j.bios.2004.04.024 Occurrence Handle1:CAS:528:DC%2BD2cXhtVKlur%2FO

    Article  CAS  Google Scholar 

  • S H Lim J Wei J Y Lin Q Li J K You (2005) ArticleTitleA glucose biosensor based on electrodeposition of palladium nanoparticles and glucose oxidase onto Nafion-solubilized carbon nanotube electrode Biosens Bioelectron 20 2341 Occurrence Handle10.1016/j.bios.2004.08.005 Occurrence Handle1:CAS:528:DC%2BD2MXis1Kjsb4%3D

    Article  CAS  Google Scholar 

  • H Sakslund J W Hammerich (1994) ArticleTitleA critical evaluation of a glucose biosensor made by codeposition of palladium and glucose oxidase on glassy carbon J Electron Chem 374 IssueID1–2 71 Occurrence Handle10.1016/0022-0728(94)03331-5 Occurrence Handle1:CAS:528:DyaK2cXlslChs7o%3D

    Article  CAS  Google Scholar 

  • T Haruyama M Aizawa (1998) ArticleTitleElectron transfer between an electrochemically deposited glucose oxidase/Cu[II] complex and an electrode Biosens Bioelectron 13 1015 Occurrence Handle10.1016/S0956-5663(98)00029-3 Occurrence Handle1:CAS:528:DyaK1cXntlSltr8%3D

    Article  CAS  Google Scholar 

  • T Gu Y Hasebe (2006) ArticleTitleDNA-Cu(II) poly(amine) complex membrane as novel catalytic layer for highly sensitive amperometric determination of hydrogen peroxide Biosens Bioelectron 21 2121 Occurrence Handle1:CAS:528:DC%2BD28XjvVWmsbs%3D

    CAS  Google Scholar 

  • X L Ren X W Meng D Chen F Q Tang J Jiao (2005) ArticleTitleUsing silver nanoparticle to enhance current response of biosensor Biosens Bioelectron 21 433 Occurrence Handle10.1016/j.bios.2004.08.052 Occurrence Handle1:CAS:528:DC%2BD2MXntVCqtrg%3D

    Article  CAS  Google Scholar 

  • S Wu H T Zhao H X Ju C G Shi J W Zhao (2006) ArticleTitleElectrodeposition of silver-DNA hybrid nanoparticles for electrochemical sensing of hydrogen peroxide and glucose Electro Commun 8 IssueID8 1197 Occurrence Handle10.1016/j.elecom.2006.05.013 Occurrence Handle1:CAS:528:DC%2BD28XotVCqt70%3D

    Article  CAS  Google Scholar 

  • Z Y Jiang Y X Yu H Wu (2006) ArticleTitlePreparation of CS/GPTMS hybrid molecularly imprinted membrane for efficient chiral resolution of phenylalanine isomers J Membrane Sci 280 876 Occurrence Handle1:CAS:528:DC%2BD28XmvFWmsrk%3D Occurrence Handle10.1016/j.memsci.2006.03.006

    Article  CAS  Google Scholar 

  • Y Miao L S Chia N K Goh S N Tan (2001) ArticleTitleAmperometric glucose biosensor based on immobilization of peroxidase in chitosan matrix crosslinked with glutaradehyde Electroanalysis 13 347 Occurrence Handle10.1002/1521-4109(200103)13:4<347::AID-ELAN347>3.0.CO;2-Z Occurrence Handle1:CAS:528:DC%2BD3MXis1KjtLY%3D

    Article  CAS  Google Scholar 

  • H Okuma E Watanabe (2002) ArticleTitleFlow system for fish freshness determination based on double multi-enzyme reactor electrodes Biosens Bioelectron 17 367 Occurrence Handle10.1016/S0956-5663(01)00309-8 Occurrence Handle1:CAS:528:DC%2BD38XhvVWht7s%3D

    Article  CAS  Google Scholar 

  • S Hikima T Kakizaki M Taga K Hasebe (1993) ArticleTitleEnzyme sensor for L-lactate with a chitosan-mercury film electrode Fresenius J Anal Chem 345 607 Occurrence Handle10.1007/BF00325809 Occurrence Handle1:CAS:528:DyaK3sXisFSrsbk%3D

    Article  CAS  Google Scholar 

  • L Chen W Gorski (2001) ArticleTitleBioinorganic composites for enzyme electrodes Anal Chem 73 2862 Occurrence Handle10.1021/ac010009z Occurrence Handle1:CAS:528:DC%2BD3MXjtlyqsbs%3D

    Article  CAS  Google Scholar 

  • B Kim W M Sigmund (2004) ArticleTitleFunctionalized multiwall carbon nanotube/gold nanoparticle composites Langmuir 20 IssueID19 8239 Occurrence Handle10.1021/la049424n Occurrence Handle1:CAS:528:DC%2BD2cXmslSnsbc%3D

    Article  CAS  Google Scholar 

  • A S Baranski T Krogulec L J Nelson (1998) ArticleTitleHigh-frequency impedance spectroscopy of platinum ultramicroelectrodes in flowing Solutions Anal Chem 70 2895 Occurrence Handle10.1021/ac9712456 Occurrence Handle1:CAS:528:DyaK1cXjvVKks7k%3D

    Article  CAS  Google Scholar 

  • J Wang L Wang S Liu (2003) ArticleTitleInteraction of K7Fe3+P2 W17O62H2 with supported bilayer lipid membranes on platinum electrode Biophys Chem 106 IssueID1 31 Occurrence Handle10.1016/S0301-4622(03)00158-3 Occurrence Handle1:CAS:528:DC%2BD3sXnsVansbs%3D

    Article  CAS  Google Scholar 

  • G A Ragoisha A S Bondarenko (2003) ArticleTitlePotentiodynamic electrochemical impedance spectroscopy. Copper underpotential deposition on gold Electrochem Commun 5 392 Occurrence Handle10.1016/S1388-2481(03)00075-4 Occurrence Handle1:CAS:528:DC%2BD3sXjs1SisLk%3D

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. State key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, China

    Ying Wang, Wanzhi Wei, Jinxiang Zeng, Xiaoying Liu & Xiandong Zeng

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  1. Ying Wang
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  2. Wanzhi Wei
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  3. Jinxiang Zeng
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  4. Xiaoying Liu
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  5. Xiandong Zeng
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Corresponding author

Correspondence to Wanzhi Wei.

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Correspondence: Wanzhi Wei, State key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China

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Wang, Y., Wei, W., Zeng, J. et al. Fabrication of a copper nanoparticle/chitosan/carbon nanotube-modified glassy carbon electrode for electrochemical sensing of hydrogen peroxide and glucose. Microchim Acta 160, 253–260 (2008). https://doi.org/10.1007/s00604-007-0844-6

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  • DOI: https://doi.org/10.1007/s00604-007-0844-6

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