Abstract
The effects of different osmotic pressure, changed by six salts (NaCl, Na2SO4, (NH4)2SO4, KH2PO4 and MSG), on cell growth and DHA synthesis by Schizochytrium sp. were investigated. Six optimal mediums were obtained to study different osmotic pressure combinations at cell growth stage and DHA synthesis stage. Results showed that cultivated cell in higher osmotic pressure condition and fermented in lower osmotic pressure condition was benefit to enhance DHA synthesis. Combination 17-6 could get the maximum cell dry weight of 56.95 g/L and the highest DHA percentage in total fatty acids of 55.21 %, while combination 17-B could get the highest lipid yield of 33.47 g/L with 42.10 % DHA in total fatty acids. This was the first report about the enhancement of DHA production by osmotic regulation and this work provided two novel osmotic control processes for high lipid yield and high DHA percentage in total fatty acids.
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References
Horrocks LA, Yeo YK (1999) Health benefits of docohexaenoic acid (DHA). Pharmacol Res 40:211–225
Rubio-Rodríguez N, Beltrán S, Jaime I, Diego SM, Sanz MT, Carballido JR (2010) Production of omega-3 polyunsaturated fatty acid concentrates: a review. Innov Food Sci Emerg. 11:1–12
Yokochi T, Honda D, Higashihara T, Nakahara T (1998) Optimization of docosahexaenoic acid production by Schizochytrium limacinum SR21. Appl Microbiol Biotechnol 49:72–76
Lang F, Busch G, Ritter M, Volkl H, Waldegger S, Gulbins E, Haussinger D (1998) Functional significance of cell volume regulatory mechanisms. Physiol Rev 78:247–306
Hohmann S (2002) Osmotic Stress Signaling and Osmoadaptation in Yeasts. Microbiol Mol Biol R. 66:300–372
Liu L, Xu Q, Li Y, Shi Z, Zhu Y, Du G, Chen J (2007) Enhancement of pyruvate production by osmotic-tolerant mutant of Torulopsis glabrata. Biotechnol Bioeng 97:825–832
Yang LB, Zhan XB, Zheng ZY, Wu JR, Gao MJ, Lin CC (2014) A novel osmotic pressure control fed-batch fermentation strategy for improvement of erythritol production by Yarrowia lipolytica from glycerol. Bioresour Technol 151:120–127
Takagi M, Karseno Yoshida T (2006) Effect of salt concentration on intracellular accumulation of lipids and triacylglyceride in marine microalgae Dunaliella cells. J Biosci Bioeng 101:223–226
Ren LJ, Ji XJ, Huang H, Qu L, Feng Y, Tong QQ, Ouyang PK (2010) Development of a stepwise aeration control strategy for efficient docosahexaenoic acid production by Schizochytrium sp. Appl Microbiol Biotechnol 87:1649–1656
Qu L, Ji XJ, Nie Ren LJ, Feng ZK, Wu WJ, Ouyang PK, Huang H (2011) Enhancement of docosahexaenoic acid production by Schizochytrium sp. using a two-stage oxygen supply control strategy based on oxygen transfer coefficient. Lett Appl Microbiol 52:22–27
Zeng Y, Ji XJ, Lian M, Ren LJ, Jin LJ, Ouyang PK, Huang H (2011) Development of a temperature shift strategy for efficient docosahexaenoic acid production by a marine fungoid protist, Schizochytrium sp. HX-308. Appl Biochem Biotechnol 164:249–255
Li X, Lin Y, Chang M, Jin Q, Wang X (2015) Efficient production of arachidonic acid by Mortierella alpina through integrating fed-batch culture with a two-stage pH control strategy. Bioresour Technol 181:275–282
Akimoto M, Ishii T, Yamagaki K, Ohtaguchi K, Koide K, Yazawa K (1991) Metal salts requisite for the production of eicosapentaenoic acid by a marine bacterium isolated from Macherel intestines. J Am Oil Chem Soc 68:504–508
Min KH, Lee HH, Anbu P, Chaulagain BP, Hur BK (2012) The effects of culture condition on the growth property and docosahexaenoic acid production from Thraustochytrium aureum ATCC 34304. Korean J Chem Eng 29:1211–1215
Ren LJ, Huang H, Xiao AH, Lian M, Jin LJ, Ji XJ (2009) Enhanced docosahexaenoic acid production by reinforcing acetyl-CoA and NADPH supply in Schizochytrium sp. HX-308. Bioprocess Biosyst Eng 32:837–843
Sun LN, Zhuang LJ, Yi X, Ji XJ, Yan JC, Huang H (2014) Differential effects of nutrient limitations on biochemical constituents and docosahexaenoic acid production of Schizochytrium sp. Bioresour Technol 159:199–206
Ren LJ, Sun GN, Ji XJ, Hu XC, Huang H (2014) Compositional shift in lipid fractions during lipid accumulation and turnover in Schizochytrium sp. Bioresour Technol 157:107–113
Wright PJ, Reed RH (1988) Method for determination of turgor pressure in macroalgae, with particular reference to the Phaeophyta. Mar Biol 99(4):473–480
Ling XP, Guo J, Liu X, Zhang X, Wang N, Lu Y, Ng IS (2015) Impact of carbon and nitrogen feeding strategy on high production of biomass and docosahexaenoic acid (DHA) by Schizochytrium sp LU310. Bioresour Technol 184:139–147
Ren LJ, Sun LN, Zhuang XY, Qu L, Ji XJ, Huang H (2014) Regulation of docosahexaenoic acid production by Schizochytrium sp.: effect of nitrogen addition. Bioprocess Biosyst Eng 37:865–872
Ren LJ, Feng Y, Li J, Qu L, Huang H (2013) Impact of phosphate concentration on docosahexaenoic acid production and related enzyme activities in fermentation of Schizochytrium sp. Bioprocess Biosyst Eng 36:1177–1183
Yan L, Bogle IDL (2007) A visualization method for operating optimization. Comput Chem Eng 31:808–814
Liu Y, Tang J, Li JJ, Daroch M, Cheng HH (2015) Efficient production of triacylglycerols rich in docosahexaenoic acid (DHA) by osmo-heterotrophic marine protists. Appl Microbiol Biotechnol 98:9643–9652
Tashima Y, Yoshimura N (1975) Control of rabbit liver fructose-1, 6-diphosphatase activity by magnesium ions. J Biochem 78:1161–1169
Lorimer GH, Badger MR, Andrews TJ (1976) The activation of ribulose-1,5-bisphosphate carboxylase by carbon dioxide and magnesium ions. Equilibria, kinetics, a suggested mechanism, and physiological implications. Biochemistry 15:529–536
Kumar S, Gummadi SN (2009) Osmotic adaptation in halotolerant yeast, Debaryomyces nepalensis NCYC 3413: role of osmolytes and cation transport. Extremophiles 13:793–805
Flahaut S, Benachour A, Giard JC, Boutibonnes P, Auffray Y (1996) Defense against lethal treatments and de novo protein synthesis induced by NaCl in Enterococcus faecalis ATCC 19433. Arch Microbiol 165:317–324
Lerudulier D, Strom AR, Dandekar AM, Smith AT, Valentine RC (1984) Moleeular biology of osmoregulation. Science 224:1064–1068
Li J, Liu RJ, Chang GF, Li XY, Chang M, Liu YF, Jin QZ, Wang XG (2015) A strategy for the highly efficient production of docosahexaenoic acid by Aurantiochytrium limacinum SR21 using glucose and glycerol as the mixed carbon sources. Bioresour Technol 177:51–57
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This work was financially supported by the National Science Foundation for Distinguished Young Scholars of China (No. 21225626), the National Natural Science Foundation of China (no. 21306085), the National Science and Technology Pillar Program (no. 2011BAD23B03), the National High Technology Research and Development Program of China (No. 2012AA021704 and No. 2014AA021701) and the Specialized Research Fund for the Doctoral Program of Higher Education (No. 20133221120008).
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Hu, XC., Ren, LJ., Chen, SL. et al. The roles of different salts and a novel osmotic pressure control strategy for improvement of DHA production by Schizochytrium sp.. Bioprocess Biosyst Eng 38, 2129–2136 (2015). https://doi.org/10.1007/s00449-015-1452-1
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DOI: https://doi.org/10.1007/s00449-015-1452-1