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Two novel myxosporean species (Myxosporea: Bivalvulida), Myxobolus marumotoi n. sp. and Cardimyxobolus japonensis n. sp., from the dark sleeper, Odontobutis obscura, in Japan

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Abstract

Two new myxosporean species, Myxobolus marumotoi n. sp. and Cardimyxobolus japonensis n. sp. (Myxozoa: Myxosporea: Bivalvulida), are described from the dark sleeper, Odontobutis obscura, in Japan, based on their morphological and molecular characterizations. Plasmodia of M. marumotoi n. sp. (Myxobolidae) grew in the myofiber of trunk muscles, forming pseudocysts. The rounded spore was relatively large, measuring 13.3–15.0 μm (average 13.8) in length, 14.2–15.0 μm (14.6) in width, and 10.0–11.7 μm (10.8) in thickness, with two subspherical polar capsules of 7.9–9.6 μm (8.4) in length by 5.4–6.3 μm (5.9) in width (n = 15). The polar capsules were directed toward the apex of the spore, packing five to six spirals of the polar filament. Plasmodia of C. japonensis n. sp. (Ortholineidae) were surrounded by thin fibrous tissue, forming cysts in the lamina propria of the alimentary tract. The spore was ovoid, wider than long, in valvular view and spindle-shaped in sutural view. It measured 8.8–10.4 μm (9.4) in length, 11.3–12.5 μm (11.9) in width, and 5.2–6.7 μm (5.8) in thickness, with two ovoid polar capsules of 4.2–5.0 μm (4.7) in length by 2.9–3.8 μm (3.3) in width (n = 15). The shell valves of spores often showed a flattened anterior border and semicircular posterior border, and the two polar capsules were directed toward opposite lateral sides. In addition, the sporoplasm contained an iodinophilous vacuole. Almost complete small-subunit (SSU) rDNA sequences, except for primer flanking regions, were obtained for both species; 1,996 bp long for the former and 1,588 bp long for the latter. On phylogenetic trees based on the SSU rDNA sequences of representative species of Bivalvulida, M. marumotoi n. sp. and C. japonensis n. sp. formed a distinct branch in the Henneguya/Myxobolus clade or near but outside this clade, respectively. This study is the first report of the genetic characterization for the genus Cardimyxobolus.

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References

  • Abdel-Ghaffar F, Abdel-Baki AA, EI Garhy M (2005) Ultrastructural characteristics of the sporogenesis of genus Myxobolus infecting some Nile fishes in Egypt. Parasitol Res 95:167–171

    Article  PubMed  Google Scholar 

  • Abdel-Ghaffar F, Ali MA, Al Quraishy S, Entzeroth R, Abdel-Baki AA, Al Farraj S, Bashtar AR (2008) Zschokkella helmii n. sp. (Myxozoa: Myxosporea), a new parasite of marbled spinefoot Siganus revulatus (Forsskal 1775), Red Sea, Egypt: light and transmission electron microscopy. Parasitol Res 102:183–192

    Article  CAS  PubMed  Google Scholar 

  • Abdel-Ghaffar F, Morsy K, Mehlhorn H, Bashtar AR, Shazly MA, Saad AH, Abdel-Gaber RA (2012) First report of Kudoa species (Myxozoa: Kudoidae) infecting the spotted coral grouper Plectropomus maculates from the Red Sea. A light and ultrastructural study. Parasitol Res 111:1579–1585

    Article  PubMed  Google Scholar 

  • Anisimova M, Gascuel O (2006) Approximate likelihood-ratio test for branches: a fast, accurate, and powerful alternative. Syst Biol 55:539–552

    Article  PubMed  Google Scholar 

  • Chen C, Ma C (1998) Fauna Sinica: Myxozoa, Myxosporea. Science Press, Beijing, p 806, 180 figure plates (in Chinese)

    Google Scholar 

  • Dereeper A, Guignon V, Blanc G, Audic S, Buffet S, Chevenet F, Dufayard JF, Guindon S, Lefort V, Lescot M, Claverie J-M, Gascuel O (2008) Phylogeny.fr: robust phylogenetic analysis for the non-specialist. Nucleic Acids Res 36:465–469

    Article  Google Scholar 

  • Easy R, Cone D (2009) Taxonomy of Myxobolus ridouti n. sp. and M. ridgwayi n. sp. (Myxozoa) from Pimephales notatus and Semotilus atromaculatus (Cypriniformes) in Ontario. J Parasitol 95:1446–1450

    Article  CAS  PubMed  Google Scholar 

  • Easy RH, Johnson SC, Cone DK (2005) Morphological and molecular comparison of Myxobolus procerus (Kudo, 1934) and M. intramusculi n. sp. (Myxozoa) parasitizing muscles of the trout-perch Percopsis omiscomaycus. Syst Parasitol 61:115–122

    Article  CAS  PubMed  Google Scholar 

  • Eiras JC, Molnár K, Lu YS (2005) Synopsis of the species of Myxobolus Bütschli, 1882 (Myxozoa: Myxosporea: Myxobolidae). Syst Parasitol 61:1–46

    Article  CAS  PubMed  Google Scholar 

  • Fiala I (2006) The phylogeny of Myxosporea (Myxozoa) based on small subunit ribosomal RNA gene analysis. Int J Parasitol 36:1521–1534

    Article  CAS  PubMed  Google Scholar 

  • Fiala I, Bartošová P (2010) History of myxozoan character evolution on the basis of rDNA and EF-2 data. BMC Evol Biol 10:228

    Article  PubMed Central  PubMed  Google Scholar 

  • Guindon S, Gascuel O (2003) A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol 52:696–704

    Article  PubMed  Google Scholar 

  • Gunter N, Adlard R (2010) The demise of Leptotheca Thélohan, 1895 (Myxozoa: Myxosporea: Ceratomyxidae) and assignment of its species to Ceratomyxa Thélohan, 1892 (Myxosporea: Ceratomyxidae), Ellipsomyxa Køie, 2003 (Myxospoea: Ceratomyxidae), Myxobolus Bütschli, 1882 and Sphaerospora Thélohan, 1892 (Myxosporea: Sphaerosporidae). Syst Parasitol 75:81–104

    Article  PubMed  Google Scholar 

  • Holzer AA, Wootten R, Sommerville C (2007) The secondary structure of the unsusually long 18S ribosomal RNA of the myxozoan Sphaerospora truttae and structural evolutionary trends in the Myxozoa. Int J Parasitol 37:1281–1295

    Article  CAS  PubMed  Google Scholar 

  • Kent ML, Andree KB, Bartholomew JL, El-Matbouli M, Desser SS, Devlin RH, Feist SW, Hedrick RP, Hoffman RW, Khattra J, Hallett SL, Lester RJG, Longshaw M, Palenzeula O, Siddall ME, Xiao C (2001) Recent advances in our knowledge of the Myxozoa. J Eukaryot Microbiol 48:395–413

    Article  CAS  PubMed  Google Scholar 

  • Li Y-C, Sato H, Kamata Y, Ohnishi T, Sugita-Konishi Y (2012) Three novel myxobolid species of genera Henneguya and Myxobolus (Myxosporea: Bivalvulida) from marine fish in Japan. Parasitol Res 111:819–826

    Article  PubMed  Google Scholar 

  • Li Y-C, Sato H, Tanaka S, Ohnish T, Kamata Y, Sugita-Konishi Y (2013) Characterization of the ribosomal RNA gene of Kudoa neothunni (Myxosporea: Multivalvulida) in tunas (Thunnus spp.) and Kudoa scomberi n. sp. in a chub mackerel (Scomber japonicus). Parasitol Res 112:1991–2003

    Article  PubMed  Google Scholar 

  • Liu Y, Whipps CM, Gu ZM, Zeng LB (2010) Myxobolus turpisrotundus (Myxosporea: Bivalvulida) spores with caudal appendages: investigating the validity of the genus Henneguya with morphological and molecular evidence. Parasitol Res 107:699–706

    Article  PubMed  Google Scholar 

  • Liu Y, Whipps CM, Gu ZM, Huang MJ, He C, Yang HL, Molnár K (2013) Myxobolus musseliusae (Myxozoa: Myxobolidae) from the gills of common carp Cyprinus carpio and revision of Myxobolus dispar recorded in China. Parasitol Res 112:289–296

    Article  CAS  PubMed  Google Scholar 

  • Lom J, Arthur JR (1989) A guideline for the preparation of species descriptions in Myxosporea. J Fish Dis 12:151–156

    Article  Google Scholar 

  • Lom J, Dyková I (2006) Myxozoan genera: definition and notes on taxonomy, life-cycle terminology and pathogenic species. Folia Parasitol 53:1–36

    Article  PubMed  Google Scholar 

  • Mansour L, Thabet A, Chourabi K, Harrath AH, Gtari M, Al Omar SY, Hassine OKB (2013) Kudoa azevedoi n. sp. (Myxozoa, Multivalvulida) from the oocystes of the Atlantic horse mackerel Trachurus trachurus (Perciformes, Carangidae) in Tunisian coasts. Parasitol Res 112:1737–1747

    Article  PubMed  Google Scholar 

  • Matsukane Y, Sato H, Tanaka S, Kamata Y, Sugita-Konishi Y (2010) Kudoa septempunctata n. sp. (Myxosporea: Multivalvulida) from an aquacultured olive flounder (Paralichthys olivaceus) imported from Korea. Parasitol Res 107:865–872

    Article  PubMed  Google Scholar 

  • Matsukane Y, Sato H, Tanaka S, Kamata Y, Sugita-Konishi Y (2011) Kudoa iwatai and two novel Kudoa spp., K. trachuri n. sp. and K. thunni n. sp. (Myxosporea: Multivalvulida) from daily consumed marine fish in western Japan. Parasitol Res 108:913–926

    Article  PubMed  Google Scholar 

  • Miller TL, Adlard RD (2013) Unicapsula species (Myxosporea: Trilosporidae) of Australian marine fishes, including the description of Unicapsula andersenae n. sp. in five teleost families off Queensland, Australia. Parasitol Res 112:2945–2957

    Article  CAS  PubMed  Google Scholar 

  • Miroshnichenko AI (1981) Neomyxobolus olae n.sp., a new species of myxosporidian (Myxosporidia, Neomyxobolidae) of the minnow. Parazitologiya 15:78–80 (in Russian with English summary)

    Google Scholar 

  • Molnár K (2011) Remarks to the validity of Genbank sequences of Myxobolus spp. (Myxozoa, Myxosporidae) infecting Eurasian fishes. Acta Parasitol 56:263–269

    Article  Google Scholar 

  • Sakai H, Iwata A, Jeon S-R (1993) Genetic evidence supporting the existence of three distinct species in the genus Odontobutis (Gobiidae) from Japan and Korea. Jpn J Ichthyol 40:61–64

    Google Scholar 

  • Sakai H, Yamamoto C, Iwata A (1998) Genetic divergence, variation and zoogeography of a freshwater goby, Odontobutis obscura. Ichthyol Res 45:363–376

    Article  Google Scholar 

  • Sato H (2011) Biology of the Myxozoa, a newly recognized parasitic pathogen causing food poisoning. Yamaguchi J Vet Med 38:1–26 (in Japanese with English summary)

    Google Scholar 

  • Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Whipps CM, Adlard RD, Bryant MS, Kent ML (2003) Two unusual myxozoans, Kudoa quadricornis n. sp. (Multivalvulida) from the muscle of goldspotted trevally (Carangoides fulvoguttatus) and Kudoa permulticapsula n. sp. (Multivalvulida) from the muscle of Spanish mackerel (Scomberomorus commerson) from the great barrier reef, Australia. J Parasitol 89:168–173

    Article  CAS  PubMed  Google Scholar 

  • Whipps CM, Grossel G, Adlard RD, Yokoyama H, Bryant MS, Munday BL, Kent ML (2004) Phylogeny of the Multivalvulidae (Myxozoa: Myxosporea) based on comparative ribosomal DNA sequence analysis. J Parasitol 90:618–622

    Article  CAS  PubMed  Google Scholar 

  • Yokoyama H, Yanagida T, Shirakashi S (2012) Kudoa ogawai n. sp. (Myxozoa: Multivalvulida) from the trunk muscle of Pacific barrelfish Hyperoglyphe japonica (Teleostei: Centrolophidae) in Japan. Parasitol Res 110:2247–2254

    Article  CAS  PubMed  Google Scholar 

  • Yurakhno VM, Ovcharenko MO, Holzer AS, Sarabeev VL, Balvuena JA (2007) Kudoa unicapsula n. sp. (Myxosporea: Kudoidae) a parasite of the Mediterranean mullets Liza ramada and L. aurata (Teleostei: Mugilidae). Parasitol Res 101:1671–1680

    Article  CAS  PubMed  Google Scholar 

  • Zhao YJ, Li NN, Tang FH, Dong JL (2013) Remarks on the validity of Myxobolus ampullicapsulatus and Myxobolus honghuensis (Myxozoa: Myxosporea) based on SSU rDNA sequences. Parasitol Res 112:3817–3823

    Article  CAS  PubMed Central  PubMed  Google Scholar 

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Acknowledgments

This study was supported in part by a Grant-in-Aid (H25-shokuhin-ippan-007) from the Ministry of Health, Labour and Welfare of Japan.

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  1. Laboratory of Veterinary Parasitology, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi, 753-8515, Japan

    Ying-Chun Li & Hiroshi Sato

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  1. Ying-Chun Li
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  2. Hiroshi Sato
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Correspondence to Hiroshi Sato.

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Li, YC., Sato, H. Two novel myxosporean species (Myxosporea: Bivalvulida), Myxobolus marumotoi n. sp. and Cardimyxobolus japonensis n. sp., from the dark sleeper, Odontobutis obscura, in Japan. Parasitol Res 113, 1371–1381 (2014). https://doi.org/10.1007/s00436-014-3776-1

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  • DOI: https://doi.org/10.1007/s00436-014-3776-1

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