Abstract
Drought is a major environmental factor that limits plant growth and productivity. After constructing a cDNA library from progressively drought-stressed hot pepper plants (Capsicum annuum), we differentially screened it to isolate cDNA clones whose expressions were drought-inducible. We named these clonesCaDSi-1, S, 7,11, and12. RNA blot analyses revealed that all transcripts of these genes were induced strongly or slightly during progressive drought stress. Their deduced amino-add sequences showed high homologies with such proteins as thioredoxinm, gtvdne-rich protein, nonspecific lipid transfer protein, oxygen-evolving enhancer protein 3, and auxin-repressed protein. In other plant species, those proteins are induced by pathogens and water or salt stresses, and are predicted to be involved in drought tolerance. The data suggest that these fiveCaDSi gene products have possible protective roles against drought stress in hot pepper.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literature Cited
Boyer JS (1982) Plant productivity and environment Science218: 443–448
Bray EA (1997) Plant responses to water deficit. Trends Plant Sci2: 45–54
Brain M, Cuine S, Peltier G, Rey P (2000) Involvement of CDSP 32, a drought-induced thioredoxin, in the response to oxidative stress in potato plants. FEBS Lett467: 245–248
Close TJ (1996) Dehydrins: Emergence of a biochemical role of a family of plant dehydration proteins. Physiol Plant97: 795–803
Frank W, Munnik T, Kerkmann K, Salamini F, Barkeis D (2000) Water deficit triggers phospholipase D activity in the resurrection plantCraterostigma plantagineum. Plant Cell12: 111–123
Hirayama T, Ohto C, Mizoguchi T, Shinozaki K (1995) A gene encoding a phosphatidylinositol-specific phospholipase C is induced by dehydration salt stress inArabidopsis thaliana. Proc Nad Acad Sci USA92: 3903–3907
Ingram J, Bartels D (1996) The molecular basis of dehydration tolerance in plants. Annu Rev Plant Physiol Plant Mol Biol47: 377–403
Issakidis-Bourguet E, Mouaheb N, Meyer Y, Miginiac-Maslow M (2001) Heterologous complementation of yeast reveals a new putative function for chloroplast m-type thioredoxin. Plant J25: 127–135
Jonak C, Kiegerl M, Ligterink W, Barker PJ, Huskisson NS, Hirt H (1996) Stress signalling in plants: A MAP kinase pathway is activated by cold drought. Proc Natl Acad Sei USA93: 11274–11279
Kang JY, Choi HI, Im MY, Kim SY (2002)Arabidopsis basic leucine zipper proteins that mediate stress-responsive abscisic acid signaling. Plant Cell14: 343–357.
Kasuga M, Liu Q, Miura S, Yamaguchi-Shinozaki K, Shinozaki K (1999) Improving plant drought, salt, and freezing tolerance by gene transfer of a single stress-inducible transcription factor. Nature Biotech17: 287–291
Lemieux B (1996) Molecular genetics of epicuticular wax biosynthesis. Trends Plant Sci1: 312–318
Mayfield SP, Bennoun P, Rochaix JD (1989) Analysis of the genes of the OEE1 OEE3 proteins of the photosystem II complex fromChlamydomonas reinhardtii. Plant Mol Biol12: 683–693
McKersie BD, Bowley SR, Harjanto E, Leprince O (1996) Water-deficit tolerance field performance of transgenic alfalfa overexpressing Superoxide dismutase. Plant Physiol111: 1177–1181
Poovaiah BW, Reddy ASN (1990) Molecular cloning sequencing of a cDNA for an auxin-repressed mRNA: Correlation between fruit growth repression of the auxin-regulated gene. Plant Mol Biol14: 127–136
Romeis T, Ludwig AA, Martin R, Jones JDG (2001) Cal-cium-dependent protein kinases play an essential role in a plant defense response. EMBO J20: 5556–5567
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning: A Laboratory Manual. 2nd ed, Cold Spring Harbor Laboratory, New York
Segura A, Moreno M, Garcia-Olmedo, F (1993) Purification antipathogenic activity of lipid transfer proteins (LTPs) from the leaves ofArabidopsis and spinach. FEBS Lett332: 243–246
Shinozaki K, Yamaguchi-Shinozaki K (1996) Molecular responses to drought and cold stress. Curr Opin Bio-technol7: 161–167
Sterk P, Booji H, Schellekens GA, van Kammen A, de Vries SC (1991) Cell-specific expression of the carrot EP2 lipid transfer protein gene. Plant Cell3: 907–921
Sugihara K, Hanagata N, Dubinsky Z, Baba S, Karube I (2000) Molecular characterization of cDNA encoding oxygen evolving enhancer proteini increased by salt treatment in the mangroveBruguiera g/mnorrhiza. Plant Cell Physiol41: 1279–1286
Yamaguchi-Shinozaki K, Shinozaki K (2001) Improving plant drought, salt, and freezing tolerance by gene transfer of a single stress-inducible transcription factor. Novartis Found Symp236: 176–186; discussion, 186–189
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Choi, K.H., Hong, C.B. & Kim, W.T. Isolation and characterization of drought-induced cDNA clones from hot pepper (Capsicum annuum). J. Plant Biol. 45, 212–218 (2002). https://doi.org/10.1007/BF03030362
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF03030362