Abstract
The sequences of the nuclear genes of the 33 kDa (OEE1) and the 16 kDa (OEE3) polypeptides of the oxygen evolving complex of Chlamydomonas reinhardtii have been established. Comparison between the OEE1 protein sequences of C. reinhardtii and higher plants and cyanobacteria reveals 67 and 47% homology. In contrast, C. reinhardtii and higher plants have only 28% overall homology for OEE3 which is mostly limited to the central portion of the protein. The transit peptides of the C. reinhardtii proteins consist of 52 (OEE1) and, most likely, 51 (OEE1) amino acids. They have a basic amino terminal region and, at least in the case of OEE1, a hydrophobic segment at their carboxy terminal end typical of thylakoid lumen proteins. Comparison of the genomic and cDNA clones indicates that the OEE1 and OEE3 genes contain five and four introns, respectively, some of which are located within the coding sequences of the transit peptides.
Similar content being viewed by others
References
Miyao M, Murata N: Photoinactivation of the oxygen-evolving complex of photosystem II. In: Kyle DJ, Osmond CB, Arntzen CJ (eds). Photoinhibition, pp. 289–307. Elsevier Science Publishers, Amsterdam (1987).
Barber J: Photosynthetic reaction centers: a common link. Trends Biochem Sci 12: 321–326 (1987).
Mayfield SP, Rahire M, Frank G, Zuber H, Rochaix JD: Expression of the nuclear gene encoding oxygen-evolving enhancer protein 2 is required for high levels of photosynthetic oxygen evolution in Chlamydomonas reinhardti. Proc Natl Acad Sci USA 84: 749–753 (1987).
Westhoff P, Jansson C, Klein-Hitpass L, Berzborn R, Larsson C, Bartlett SG: Intracellular coding sites of polypeptides associated with photosynthetic oxygen evolution of photosystem II. Plant Mol Biol 4: 137–146 (1985).
Ljungberg U, Akerlund HE, Andersson B: Isolation and characterization of the 10 kDa and 22 kDa polypeptides of higher plant photosystem II. Eur J Biochem 158: 477–482 (1986).
Ljungberg U, Henrysson T, Rochester CP, Akerlund HE, Andersson B: The presence of low-molecular-weight polypeptides in spinach Photosystem II core preparations. Isolation of a 5 kDa hydrophilic polypeptide. Biochim Biophys Acta 849: 112–120 (1986).
Packham NK: Is the 9 kDa thylakoid membrane phosphoprotein functionally and structurally analogous to the H subunit of bacterial reaction centers? FEBS Lett 231: 284–290 (1988).
Critchley C: The role of chloride in Photosystem II. Biochim Biophys Acta 811: 33–46 (1985).
Andersson B: Protein participating in photosynthetic water oxidation. Encycl Plant Physiol 19: 447–456 (1986).
Mayfield SP, Bennoun P, Rochaix JD: Expression of the nuclear encoded OEE1 protein is required for oxygen evolution and stability of photosystem II particles in Chlamydomonas reinhardtii. EMBO J 6: 313–318 (1987).
Oh-oka H, Tanaka S, Wada K, Kuwabara T, Murata N: Complete amino acid sequence of 33 kDa protein isolated from spinach photosystem II particles. FEBS Lett 197: 63–66 (1986).
Tyagi A, Hermans J, Steppuhn J, Jansson Ch, Vater F, Herrmann RG: Nucleotide sequence of cDNA clones encoding the complete 33 kDa precursor protein associated with the photosynthetic oxygen-evolving complex from spinach. Mol Gen Genet 207: 288–293 (1987).
Jansen T, Rother C, Steppuhn J, Reinke H, Beyreuther K, Jansson C, Andersson B, Herrmann RG: Nucleotide sequence of cDNA clones encoding the complete 23 kDa and 16 kDa precursor proteins associated with the photosynthetic oxygen-evolving complex from spinach. FEBS Lett 216: 234–240 (1987).
Schmidt GW, Devilliers-Thiery A, Desruisseaux H, Blobel G, Chua NH: NH terminal sequences of precursor and mature forms of the ribulose-1,5-bisphosphate carboxylase small subunit from Chlamydomonas reinhardtii. J Cell Biol 83: 615–622 (1979).
Goldschmidt-Clermont M, Rahire M: Sequence, evolution and differential expression of the two genes encoding variant small subunits of ribulose bisphosphate carboxylase/oxygenase in Chlamydomonas reinhardtii. J Mol Biol 191: 421–432 (1986).
Young RA, Davis RW: Efficient isolation of genes by using antibody probes. Proc Natl Acad Sci USA 80: 1194–1198 (1983).
Benton WD, Davis RW: Screening λgt recombinant clones by hybridization to single plaques in situ. Science 196: 180 (1977).
Goldschmidt-Clermont M: The two genes for the small subunit of RuBP carboxylase/oxygenase are closely linked in Chlamydomonas reinhardtii. Plant Mol Biol 6: 13–21 (1986).
Maxam AM, Gilbert W: Sequencing end-labelled DNA with base-specific chemical cleavages. Meth Enzymol 64: 499–560 (1980).
Berk AJ, Sharp PA: Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease digested hybrids. Cell 12: 721–732. (1977).
Rochaix JD: Restriction fragments from Chlamydomonas chloroplast DNA. Meth Enzymol 65: 785–795 (1980).
Khandjian EW: UV crosslinking of RNA to nylon membrane enhances hybridization signals. Mol Biol Rep 11: 107–115 (1986).
Johnson DA, Gantsch JW, Sportman JR, Elder JH: Gene. Anal Tech 1: 3–8 (1984).
Chua NH, Matlin K, Bennoun P: A chlorophyll-protein complex lacking in photosystem II mutants of Chlamydomonas reinhardtii. J Cell Biol 67: 361–377 (1975).
Delepelaire P: Partial characterization of the biosynthesis and integration of the photosystem II reaction centers in the thylakoid membrane of Chlamydomonas reinhardtii. EMBO J 3: 701–706 (1984).
Smeekens S, deGroot M, vanBinsbergen J, Weisbeek P: Sequence of the precursor of the chloroplast thylakoid lumen protein plastocyanin. Nature 317: 456–458 (1985).
Rother C, Jansen T, Tyagi A, Tittgen J, Herrmann RG: Plastocyanin is encoded by an uninterrupted nuclear gene in spinach. Curr Genet 11: 171–176.
Silflow CD, Chisholm RL, Conner TW, Ranum PW: The two alpha-tubulin genes of Chlamydomonas reinhardtii code for slightly different proteins. Mol Cell Biol 5: 2389–2398.
Philbrick J, Zilinskas BA: Cloning, nucleotide sequence and mutational analysis of the gene encoding the Photosystem II manganese-stabilizing polypeptide of Synechocystis 6803. Mol Gen Genet 212: 418–425 (1988).
Kuwabara T, Teddy KJ, Sherman LA: Nucleotide sequence of the gene from the cyanobacterium Anacystis nidulans R2 encoding the Mn-stabilizing protein involved in photosystem II water oxidation. Proc Natl Acad Sci USA 84: 8230–8234 (1987).
Erickson JM, Rochaix JD, Delepelaire P: Analysis of genes encoding two photosystem II proteins of the 30–34 kD size class. In: Steinback B, Bonitz S, Arntzen CJ, Bogorad L (eds). Molecular Biology of the Photosynthetic Apparatus, pp. 53–65. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (1985).
Rochaix JD, Kuchka M, Mayfield SP, Schirmer-Rahire M, Girard-Bascou J, Bennoun P: Nuclear and chloroplast mutations affect the synthesis or stability of the chloroplast psbC gene product in Chlamydomonas reinhardtii. EMBO J, in press.
Robinson C, Ellis RJ: Transport of proteins into chloroplasts. Eur J Biochem 142: 237–342 (1984).
Chia CP, Arntzen CJ: Evidence for two-step processing of nuclear-encoded chloroplast proteins during membrane assembly. J Cell Biol 103: 725–731 (1986).
Hageman J, Robinson C, Smeekens S, Weisbeek P: A thylakoid processing protease is required for complete maturation of the lumen protein plastocyanin. Nature 324: 567–569 (1986).
Brändén CI, Eklund H, Cambillou C, Pryor AJ: Correlation of exons with structural domains in alcohol dehydrogene. EMBO J 3: 1307–1310 (1984).
Needleman SB, Wunsch CD: A general method applicable to the search for similarities in the amino acid sequence of two proteins. J Mol Biol 48: 443–453 (1970).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mayfield, S.P., Schirmer-Rahire, M., Frank, G. et al. Analysis of the genes of the OEE1 and OEE3 proteins of the photosystem II complex from Chlamydomonas reinhardtii . Plant Mol Biol 12, 683–693 (1989). https://doi.org/10.1007/BF00044159
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00044159