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Lectins from seeds of jack fruit (Artocarpus integrifolia L.): isolation and purification of two isolectins from the albumin fraction

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Biologia Plantarum

Abstract

Two isolectins were isolated from the albumin fraction ofArtocarpus integrifolia L. seeds, by precipitation with ammonium sulfate, and DEAE-cellulose and SP-Sephadex C-50 chromatography. The isolectins, when passed through Sephadex G-100 at pH 7.4, had a molecular mass of 43 000 and when subjected to SDS electrophoresis in the presence of β-mercaptoethanol consisted of two subunits with molecular mass of 11 250 and 15 000. When they were tested with human erythrocytes of all the groups of the ABO system there was no blood specificity. The isolectins formed interference arcs by Ouchterlony double diffusion in agarose gel.

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Abbreviations

DEAE-cellulose:

Diethylaminoethyl-cellulose

SDS:

sodium dodeoyl sulfate

SP-Sephadex:

sulphopropyl-Sephadex

References

  • Boyd, W. C.: The lectins: their present status. -Vox Sang.8: 1–32, 1963.

    PubMed  CAS  Google Scholar 

  • Clarke, J. T.: Simplified “dise” (polyaerylamide gel) electrophoresis. -Ann. N. Y. Acad. Sci.121 Art. 2: 428–436, 1964.

    Article  PubMed  CAS  Google Scholar 

  • Clausen, J.: Immunochemical Techniques for the Identification and Estimation of Macromolecules. -North-Holland Publ., Amsterdam 1969.

    Google Scholar 

  • Dahlgreen, K., Porath, J., Lindahl-Kiessling, K.: On the purification of a phytohemagglutinins fromPhaseolus vulgaris seeds. -Arch. Biochem. Biophys.137: 306–314, 1970.

    Article  Google Scholar 

  • Determann, H., Michel, W.: The correlation between molecular weight and elution behaviour in the gel chromatography of proteins. -J. Chromatogr.25: 303–313, 1966.

    Article  CAS  Google Scholar 

  • Goa, J.: A micro biuret method for protein determination. Determination of total protein in cerebrospinal fluid. -Scand. J. Clin. Lab. Invest.5: 218–222, 1953.

    Article  PubMed  CAS  Google Scholar 

  • Jaffé, W. G.: Hemagglutinins. -In:Liener, I. E. (ed.): Toxic Constituents of Plant Foodstuffs. Pp. 69–74. Academic Press, New York 1969.

    Google Scholar 

  • Kauss, H.: Plant lectins (Phytohemagglutinins). -Progr. Bot.38: 58–70, 1977.

    Google Scholar 

  • Liener, I. E.: Phytohemagglutinins (Phytolectins). -Annu. Rev. Plant Physiol.27: 29L to 319, 1976.

    Article  Google Scholar 

  • Monsigny, M., Sampaio Filho, J. C., Duarte, J. H.: Purification d’une lectine du haricot blanc (Phaseolus vulgaris). -Arq. Biol. Tecnol.15: 40–41, 1972.

    CAS  Google Scholar 

  • Moreira, R. A., Perrone, J. C.: Purification and partial characterization of a lectin fromPhaseolus vulgaris.-Plant Physiol.59: 783–787, 1977.

    Article  PubMed  CAS  Google Scholar 

  • Olsnes, S., Pappenheiner, A. M. Jr.,Meren, R.: Lectins fromAbrus precatorius andRicinus communis. II. Hybrid toxins and their interaction with chain-specific antibodies. -J. Immunol.113: 842–847, 1974.

    PubMed  CAS  Google Scholar 

  • Pappenheiner, A. M. Jr.,Olsnes, S., Harper, A. A.: Lectins fromAbrus precatorius andRicinus communis. I. Immunochemical relationships between toxins and agglutinins. -J. Immunol.113: 835 to 841, 1974.

    Google Scholar 

  • Pusztai, A., Steward, J. C.: Isolectins ofPhaseolus vulgaris. Physicochemical studies. -Biochim. biophys. Acta536: 38–49, 1978.

    PubMed  CAS  Google Scholar 

  • Reisfeld, R. A., Lewis, U. J., Williams, D. E.: Disk electrophoresis of basic proteins and peptides on polyacrylamide gels. -Nature195: 281–283, 1962.

    Article  PubMed  CAS  Google Scholar 

  • Rice, R. H.: Wheat germ agglutinin. Evidence for a genetic basis of multiple forms. -Biochim. biophys. Acta444: 175–180, 1976.

    PubMed  CAS  Google Scholar 

  • Rougé, P.: Interactions entre les protéines du serum humain normal et les hémagglutinines des graines de Pois. -Comp. rend. Acad. Sci. Paris (Sér. D)283: 1823–1825, 1976.

    Google Scholar 

  • Toms, G. C., Western, A.: Phytohaemagglutinins. -In:Arborne, J. B., Boulter, D., Turner, B. L. (ed.): Chemotaxonomy ofLeguminosae. Pp. 367–456. Academic Press, New York 1971.

    Google Scholar 

  • Walhneldt, T. V.: Sodium dodecyl sulfate in protein. -BioSystems6: 167–187, 1975.

    Google Scholar 

  • Weber, K., Osborne, M.: The reliability of molecular weight determinations by dodecyl sulfate polyacrylamide gel electrophoresis. -J. biol. Chem.244: 4406–4412, 1969.

    PubMed  CAS  Google Scholar 

  • Xavier Filho, J., Moreira, R. A.: Visualization of proteinase inhibitors in SDS-polyacrylamide gels. -Anal. Biochem.84: 296–303, 1978.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, Ceará, Brazil

    R. de Azevedo Moreira & Iracema Lima Ainouz

  2. Caixa Postal 1065, 60000, Fortaleza, Ceará, Brazil

    R. de Azevedo Moreira

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  1. R. de Azevedo Moreira
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  2. Iracema Lima Ainouz
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de Azevedo Moreira, R., Ainouz, I.L. Lectins from seeds of jack fruit (Artocarpus integrifolia L.): isolation and purification of two isolectins from the albumin fraction. Biol Plant 23, 186–192 (1981). https://doi.org/10.1007/BF02894883

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  • DOI: https://doi.org/10.1007/BF02894883

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