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Antibacterial and Hemolytic Activity of a new Lectin Purified from the Seeds of Sterculia Foetida L.

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Abstract

The aim of this study was to isolate, characterize, and verify possible antibacterial and hemolytic activity for a lectin found in the seeds of Sterculia foetida L. Purification of the lectin from S. foetida (SFL) was realized with ion exchange chromatography DEAE-Sephacel coupled to HPLC. The purity and the molecular weight was determined by SDS-PAGE. The isolated SFL was characterized as to its glycoprotein nature, and sugar specificity, as well as resistance to pH, temperature, denaturing agents, reduction, oxidation, and chelation. A microdilution method was used to determine antibacterial activity, and hemolytic activity was observed in human erythrocytes. The SFL has a molecular weight of 17 kDa, and a carbohydrate content of 53 μg/mL, specific for arabinose and xylose, and is resistant to treatment with urea, sensitive to treatment with sodium metaperiodate and β-mercaptoethanol, and in the presence of EDTA lost its hemagglutinating activity (HA). However, in the presence of divalent cations (Ca2 + and Mn2 +) the HA was increased. The SFL remained active even after incubation at 80 °C, and, within pH values of between 5 and 11. The SFL inhibited the bacterial growth of all the tested strains and caused little hemolysis in human erythrocytes when compared to the positive control Triton X-100.

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Authors and Affiliations

  1. Department of Molecular Biology, Federal University of Paraíba, Campus I, João Pessoa, Paraíba, CEP 58059-900, Brazil

    Alana Araújo Braga, Rodrigo Rodrigues e Lacerda, Gracy Kelly Vieira de Vasconcelos Medeiros, Gregório Fernandes Gonçalves, Hilzeth de Luna Freire Pessoa, Juscélio Donizete Cardoso, Carlos Alberto de Almeida Gadelha, Bagnólia Araújo da Silva & Tatiane Santi-Gadelha

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  1. Alana Araújo Braga
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  2. Rodrigo Rodrigues e Lacerda
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  3. Gracy Kelly Vieira de Vasconcelos Medeiros
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Correspondence to Tatiane Santi-Gadelha.

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Braga, A.A., e Lacerda, R.R., de Vasconcelos Medeiros, G.K.V. et al. Antibacterial and Hemolytic Activity of a new Lectin Purified from the Seeds of Sterculia Foetida L.. Appl Biochem Biotechnol 175, 1689–1699 (2015). https://doi.org/10.1007/s12010-014-1390-4

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