Skip to main content
Log in

Frequent duplication and deletion events in the 5S RNA genes and the associated spacer regions of theTriticeae

  • Published:
Plant Systematics and Evolution Aims and scope Submit manuscript

Abstract

The 5 S DNA units from 15 grasses in theTriticeae were analysed at the DNA sequence level. Four units carried duplications near the 3′-end of the 5 S RNA gene with 3 of the duplications centred on the same base pairs as a duplication previously reported byGerlach & Dyer. The fourth duplication was located 3′ downstream from the gene, in the spacer region. Apparent deletions were very frequent when units of the different grasses were compared and it was clear that these deletions did not extend into a 75 bp spacer region upstream from the 5 S RNA gene. This 75 bp region also tended to be more conserved between the grasses as compared to the high level of sequence change in the rest of the spacer region. — Phenetic relationships were established between the grasses using the sequence data. The relationships were generally consistent with the data from other parameters and, in addition, showed that two Australian grasses were closely related to the other Northern hemisphere genera examined. The data concerning the Australian grasses is discussed in relation to the isolated nature of Australia.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Appels, R., Dvoŕak, J., 1982: The wheat ribosomal DNA spacer region: its structure and variation in populations and among species. — Theor. Appl. Genet.63: 337–348.

    Google Scholar 

  • —,Moran, L. B., 1984: Molecular analysis of alien chromatin introduced into wheat. — Stadler Genet. Symp.16: 529–557.

    Google Scholar 

  • —,Gerlach, W. L., Dennis, E. S., Swift, H., Peacock, W. J., 1980: Molecular and chromosomal organization of DNA sequences coding for the ribosomal RNA's in cereals. — Chromosoma78: 293–311.

    Google Scholar 

  • Arnheim, N., 1983: Concerted evolution of multigene families. — InNei, M., Koehn, R. K., (Eds.): Evolution of genes and proteins, pp. 38–61. — Sunderland, Mass.: Sinauer Associates Inc.

    Google Scholar 

  • Audley-Charles, M. G., Hurley, A. M., Smith, A. G., 1981: Continental movements in the Mesozoic and Cenozoic. — InWhitmore, T. C., (Ed.): Wallace's line and plate tectonics, pp. 9–23. — Oxford: Clarendon Press.

    Google Scholar 

  • Bogenhagen, D. F., Brown, D. D., 1981: Nucleotide sequences inXenopus 5 S DNA required for transcription termination. — Cell24: 261–270.

    Google Scholar 

  • Brettell, R. I. S., Pallotta, M. A., Gustafson, J. P., Appels, R., 1986: Variation at theNor loci in triticale derived from tissue culture. — Theor. Appl. Genet.71: 637–643.

    Google Scholar 

  • Brown, R. D., Brown, D. D., 1976: The nucleotide sequence adjoining the 3′-end of the genes coding for three oocyte-type 5 S ribosomal RNA inXenopus. — J. Mol. Biol.102: 1–14.

    Google Scholar 

  • Clayton, W. D., Renvoize, S. A., 1986: Genera Graminum; grasses of the world. — Kew Bull. Addit. Ser.13: 1–27.

    Google Scholar 

  • Craw, R. C., Grehan, J. R., Heads, M. J., 1984: Croizat's panbiogeography and principia botanica—series of papers edited byR. C. Craw andG. W. Gibbs. — Tuatara27: 1–74.

    Google Scholar 

  • Dewey, D. R., 1984: The genomic system of classification as a guide to intergeneric hybridization with the perennialTriticeae. — Stadler Genet. Symp.16: 209–280.

    Google Scholar 

  • Donn, G., Tischer, E., Smith, J. E., Goodman, H., 1984: Herbicide-resistant alfalfa cells: an example of gene amplification in plants. — J. Mol. Appl. Genet.2: 621–633.

    Google Scholar 

  • Dvorák, J., 1980: Homoeology betweenAgropyron elongatum chromosomes andTriticum aestivum chromosomes. — Canad. J. Genet. Cytol.22: 237–259.

    Google Scholar 

  • —,Kasarda, D. D., Dietler, M. D., Lew, E. J. L., Anderson, O. D., Litts, J. C., Shewry, P. R., 1986: Chromosomal location of seed storage protein genes in the genome ofElytrigia elongata. — Canad. J. Genet. Cytol.28: 831–834.

    Google Scholar 

  • Efstradiatis, A., Posakony, J. W., Maniatis, T., Lawn, R. M., O'Connell, C. O., Spritz, R. A., De Riel, J. K., Forget, B. G., Weissman, S. M., Slighton, J. L., Blechl, A. E., Smithies, O., Baralle, F. E., Shoulders, C. C., Proudfoot, N. J., 1980: The structure and evolution of the human β-globin gene family. — Cell21: 653–668.

    Google Scholar 

  • Endow, S. A., Komma, D. J., 1986: One-step and stepwise magnification of a bobbed lethal chromosome inDrosophila melanogaster. — Genetics114: 511–523.

    Google Scholar 

  • Erdmann, V. A., Wolters, J., 1986: Collection of published 5 S, 5.8 S and 4.5 S ribosomal RNA sequences. — Nucleic Acids Res.14: Supplement.

  • Fedoroff, N. V., 1979: On spacers. — Cell16: 697–710.

    Google Scholar 

  • —,Brown, D. D., 1978: The nucleotide sequence of oocyte 5 S DNA inXenopus laevis. The AT-rich spacer. — Cell13: 701–716.

    Google Scholar 

  • Gerlach, W. L., Dyer, T. A., 1980: Sequence organisation of the repeating units in the nucleus of wheat which contain 5 S RNA genes. — Nucleic Acids Res.8: 4851–4865.

    Google Scholar 

  • Gill, B. S., 1981: Evolutionary relationships based heterochromatin bands in six species of theTriticinae. — J. Heredity72: 391–394.

    Google Scholar 

  • —,Appels, R., 1988: Relationships betweenNor-loci from differentTriticeae species. — Pl. Syst. Evol.160: 77–89.

    Google Scholar 

  • —,Morris, K. L. D., Appels, R., 1988: Assignment of the genomic affinities of chromosomes from polyploidElymus species added to wheat. — Genome30: 70–82.

    Google Scholar 

  • Goldsborough, R. B., Ellis, T. H. N., Lomonossoff, G. D., 1982: Sequence variation and methylation of the flax 5 S RNA genes. — Nucleic Acids Res.10: 4501–4514.

    Google Scholar 

  • Hsaio, C., Wang, R. R.-C., Dewey, D. R., 1986: Karyotype analysis and genome relationships of 22 diploid species in the tribeTriticeae. — Canad. J. Gen. Cytol.28: 109–120.

    Google Scholar 

  • Kay, B. K., Gall, J. G., 1981: Ribosomal RNA genes of the newtNotophthalamus viridescens. — Nucleic Acids Res.9: 6457–6465.

    Google Scholar 

  • Koebner, R. M. D., Appels, R., Shepherd, K. W., 1986: Rye heterochromatin II. Characterization of a derivative from chromosome 1 DS. IRL with a reduced amount of the major repeating sequence. — Canad. J. Genet. Cytol.28: 658–664.

    Google Scholar 

  • Kota, R. S., Dvorák, J., 1986: Mapping of a chromosome pairing gene and 5 S RNA genes inTriticum aestivum L. by a spontaneous deletion in chromosome arm 5 Bp. — Canad. J. Genet. Cytol.28: 266–271.

    Google Scholar 

  • Lassner, M. W., Dvorák, J., 1985: Organisation of the 5 S RNA gene family in wheat. — J. Cell Biochem. (Suppl.)96: 219.

    Google Scholar 

  • Lawrence, G. J., Appels, R., 1986: Mapping the nucleolus organizer region, seed protein loci and isozyme loci on chromosome 1 R in rye. — Theor. Appl. Genet.71: 742–749.

    Google Scholar 

  • Long, E. O., Dawid, I. B., 1980: Repeated genes in eukaryotes. — Ann. Rev. Biochem.43: 727–764.

    Google Scholar 

  • Lu, A.-L., Steege, D. A., Stafford, D. W., 1980: Nucleotide sequence of a 5 S ribosomal RNA gene in the sea urchinLytechinus variegatus. — Nucleic Acids Res.8: 1839–1853.

    Google Scholar 

  • Maan, S. S., 1975: Cytoplasmic variability and speciation inTriticinae. — InWali, M. K., (Ed.): Prairie: A multiple view, pp. 255–281. — Grand Forks: The Univ. of N. Dakota Press.

    Google Scholar 

  • Maxam, A. M., Tizard, R., Skryabin, K. G., Gilbert, W., 1977: Promoter region for yeast 5 S ribosomal RNA. — Nature267: 643–645.

    Google Scholar 

  • Morris, R., Sears, E. R., 1967: The cytogenetics of wheat and its relatives. — InQuisenbury, O., Reitz, L. P., (Eds.): Wheat improvement monograph13: 31–50.

    Google Scholar 

  • Nevers, P., Shepherd, N. S., Saedler, H., 1986: Plant transposable elements. — Advances Bot. Research12: 103–203.

    Google Scholar 

  • Nix, H. A., 1982: Environmental determinants of biogeography and evolution in Terra Australis. — InBarker, W. R., Greenslade, P. J. M., (Eds.): Evolution of the flora and fauna of arid Australia, pp. 47–66. — South Australia: Peacock publications.

    Google Scholar 

  • Rafalski, J. A., Wiewiorowski, M., Söll, D., 1982: Characterisation and nucleotide sequence of nuclear 5 S RNA genes in yellow lupin (Lupinus luteus). — Nucleic Acids Res.10: 7635–7642.

    Google Scholar 

  • Rothschild, L. J., Ragan, M. A., Coleman, A. W., Heywood, P., Gerbi, S. A., 1986: Are rRNA sequence comparisons the Rosetta Stone of phylogenetics. — Cell47: 640–641.

    Google Scholar 

  • Rubacha, A., Sumner, III, W., Richter, L., Beckingham, K., 1984: Conserved 5′ flank homologies in dipteran 5 S RNA genes that would function on “A” from DNA. — Nucleic Acids Res.12: 8193–8207.

    Google Scholar 

  • Sears, E. R., 1953: Addition of the genome ofHaynaldia villosa toTriticum aestivum. — Amer. J. Bot.40: 168–173.

    Google Scholar 

  • —, 1969: Wheat cytogenetics. — Ann. Rev. Genet.3: 451–468.

    Google Scholar 

  • Smith, D. K., 1987: Concept and use of software for pair-wise and multiple genetic sequence alignment. — Technical Report No.11, Canberra CAE, ACT Australia.

  • Truswell, E. M., Harris, W. K., 1982: The Cainozoic palaeobotanical record in arid Australia: fossil evidence for the origins of an arid-adapted flora. — InBarker, W. R., Greenslade, P. J. M., (Eds.): Evolution of the flora and fauna of arid Australia, pp. 67–76. — South Australia: Peacock publications.

    Google Scholar 

  • Tschudi, C., Pirrotta, V., 1980: Sequence and heterogeneity in the 5 S RNA gene cluster ofDrosophila melanogaster. — Nucleic Acids Res.8: 441–451.

    Google Scholar 

  • Valenzuela, P., Bell, G. I., Masiarz, F. R., De Gennaro, L. J., Rutter, W. J., 1977: Nucleotide sequence of the yeast 5 S ribosomal RNA gene and adjacent putative control regions. — Nature267: 641–643.

    Google Scholar 

  • Vandenberghe, A., Chen, M.-W., Dams, E., de Baere, R., de Roeck, E., Huysmans, E., de Wachter, R., 1984: The corrected nucleotide sequences of 5 S RNA's from six angiosperms. — FEBS Lett.171: 17–23.

    Google Scholar 

  • Xin, Z.-Y., Appels, R., 1988: Occurrence of rye (Secale cereale) 350-family DNA sequences inAgropyron and otherTriticeae. — Pl. Syst. Evol.160: 65–76.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Scoles, G.J., Gill, B.S., Xin, Z.Y. et al. Frequent duplication and deletion events in the 5S RNA genes and the associated spacer regions of theTriticeae . Pl Syst Evol 160, 105–122 (1988). https://doi.org/10.1007/BF00936713

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00936713

Key words

Navigation