Abstract
Informational suppressors and antisuppressors have been previously isolated in Podospora anserina, and their properties suggest that they could be ribosomal mutants involved in the control of translational fidelity. In this paper we present results concerning relationships between these mutants and paromomycin, an aminoglycoside antibiotic known to stimulate translational errors. The mutants were found to manifest an altered growth sensitivity to this drug as compared with the wild-type strain: Most of the suppressors were more sensitive and, in contrast, most of the antisuppressors were more resistant to paromomycin. Moreover, phenotypic suppression of an auxotrophic mutation by paromomycin was observed only if a suppressor and an antisuppressor had been introduced in the strain. These results suggest that ambiguity levels could be altered in the suppressor and antisuppressor strains. In addition, paromomycin was shown to abolish sporulation, which suggests relationships between mistranslation and a step of cellular differentiation.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bayliss, F. T., and Vinopal, R. T. (1971). Selection of ribosomal mutants by antibiotic suppression in yeast. Science 1741339.
Chattoo, B. B., Palmer, E., Ono, B. I., and Sherman, F. (1979). Pattern of genetic and phenotypic suppression of lys2 mutations in the yearst Saccharomyces cerevisiae. Genetics 9367.
Coppin-Raynal, E. (1977). Ribosomal suppressors and antisuppressors in Podospora anserina: Resistance to cycloheximide. J. Bacteriol. 131876.
De Wilde, M., Cabezon, T., Herzog, A., and Bollen, A. (1977). Apport de la génétique à la connaissance du ribosome bactérien. Biochimie 59125.
Dequard, M. (1980). Recherche de mutants ribosomiques chez le champignon Podospora anserina: Analyse de mutants resistants et hypersensibles à la paromomycine. Thèse de 3ème Cycle, Université Paris-Sud, Centre d'Orsay, France.
Dequard, M., Couderc, J. L., Legrain, P., Belcour, L., and Picard-Bennoun, M. (1980). Search for ribosomal mutants in Podospora anserina: Genetic analysis of mutants resistant to paromomycin. 18:263.
Elseviers, D., and Gorini, L. (1975). Direct selection of mutants restricting efficiency of suppression and misreading levels in E. coli B. Mol. Gen. Genet. 137177.
Esser, K. (1974). In King, R. C. (ed.), Handbook of Genetics, Vol. 1 Plenum Press, New York and London, pp. 531–551.
Garvin, R. T., and Gorini, L. (1975). A new gene for ribosomal restriction in Escherichia coli. Mol. Gen. Genet. 13773.
Gorini, L. (1974). Streptomycin and misreading of the genetic code. In Nomura, N., Tissières, A., and Lengyel, P., (eds.), Ribosomes Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y., p. 791.
Gorini, L., Jacoby, G., and Breckenridge, L. (1966). Ribosomal ambiguity. Cold Spring Harbor Symp. Quant. Biol. 31657.
Palmer, E., and Wilhelm, J. M. (1978). Mistranslation in an eukaryotic organism. Cell 13329.
Palmer, E., Wilhelm, J., and Sherman, F. (1979). Phenotypic suppression of nonsense mutants in yeast by aminoglycoside antibiotics. Nature 277148.
Picard, M. (1973). Genetic evidence for a polycistronic unit of transcription in the complex locus “14” in Podospora anserina. II. Genetic analysis of informational suppressors. Genet. Res. (Cambr.) 211.
Picard-Bennoun, M. (1976). Genetic evidence for ribosomal antisuppressors in Podospora anserina. Mol. Gen. Genet. 147299.
Picard-Bennoun, M., and Coppin-Raynal, E. (1977). Une fonction ribosomique essentielle: Le contrôle de la fidélité de la traduction Physiol. Veg. 15481.
Piepersberg, W., Geyl, D., Hummel, H., and Böck, O. (1980). Physiology of ribosomal mutants. Physiology and biochemistry of bacterial ribosomal mutants. Genetics and evolution of transcriptional and translational apparatus Kodanska Scientific, Tokyo, p. 359.
Rizet, G., and Engelmann, C. (1949). Contribution à l'étude génétique d'un ascomycète tétra-sporé: Podospora anserina. Rev. Cytol. Biol. Veg. 2202.
Rosset, R., and Gorini, L. (1969). A ribosomal ambiguity mutation. J. Mol. Biol. 3995.
Singh, A., Ursic, D., and Davies, J. (1979). Phenotypic suppression and misreading in Saccharomyces cerevisiae. Nature 277146.
Surguchov, A. P., Berestetskaya, Yu, V., Fominykch, E. S., Pospelova, E. M., Smirnov, V. N., Ter-Avanesyan, M. D., and Ingé-Vechtomov, S. G. (1980). Recessive suppression in yeast Saccharomyces cerevisiae is mediated by a ribosomal mutation. FEBS Lett. 111175.
Wilhelm, J. M., Jessop, J. J., and Pettitt, S. E. (1978a). Aminoglycoside antibiotics and eukaryotic protein synthesis: Stimulation of errors in the translation of natural messengers in extracts of cultured human cells. Biochemistry 171149.
Wilhelm, J. M., Pettitt, S. E., and Jessop, J. J., (1978b). Aminoglycoside antibiotics and eukaryotic protein synthesis: Structure-function relationships in the stimulation of misreading with a wheat embryo system. Biochemistry 17(71143
Author information
Authors and Affiliations
Additional information
This work was supported by a DGRST grant and by a NATO grant.
Rights and permissions
About this article
Cite this article
Coppin-Raynal, E. Ribosomal suppressors and antisuppressors in Podospora anserina: Altered susceptibility to paromomycin and relationships between genetic and phenotypic suppression. Biochem Genet 19, 729–740 (1981). https://doi.org/10.1007/BF00484005
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00484005