Abstract
A series of human neuroectodermal tumors, all containing more than the normal diploid DNA, and each with its own distinct chromosome mode, were studied using restriction enzyme cleavage and specific DNA sequence hybridization. Methods described were quite sensitive and quantitative and as few as 40 molecules with a given restriction site were reproducibly detected in total nuclear DNA. Analysis of several fluorescent gel bands associated with different chromosomal domains revealed no changes between any of the tumor and normal cells. Specific probe hybridization, using purified complex repeating sequences, indicated fidelity of base sequence, as well as preservation of the relative amounts of each of a number of minor related multimers in both the tumor and normal cells. Centromeric regions containing arrays of such sequences may be maintained in these tumor cells and furthermore it is possible that some of these cells are polyploid with respect to DNA sequences, rather than aneuploid as their chromosome profiles suggest.
Similar content being viewed by others
References
Atkin, N. B.: Chromosomes in human malignant tumors: a review and assessment, In: Chromosomes and cancer (J. German, ed.), p. 375–422. New York: J. Wiley 1974
Balaban-Malenbaum, G., Gilbert, F.: Double minute chromosomes and the homogenously staining regions in chromosomes of a human neuroblastoma cell line. Science 198, 739–741 (1977)
Cooke, H.: Repeated sequence specific to human males. Nature (Lond.) 262, 182–186 (1976)
Deaven, L.L., Sanders, P.C., Grilly, J.L., Kraemer, P.M., Petersen, D.: Chromosome G-banding and DNA constancy in aneuploid cell populations, In: Mammalian cells: probes and problems (C.R. Richmond et al., eds.), p. 212–227. Oak Ridge, Tenn.: Technical Information Center 1975
Francke, V.: Retinoblastoma and chromosome 13. Cytogenet. Cell Genet. 16, 131–134 (1976)
Frederiksen, P., Reske-Nielsen, E., Bichel, P.: Flow cytometry in tumours of the brain. Acta neuropath. (Berl.) 41, 179–183 (1978)
Harnden, P.G.: Cytogenetics of human neoplasia, In: Genetics of human cancer (J.J. Mulvilhill, R.W. Miller, J.F. Fravmeni, eds.), p. 87–104. New York: Raven Press 1977
Jones, K.W., Purdom, I.F., Prosser, J., Corneo, G.: The chromosomal location of human satellite I DNA. Chromosoma (Berl.) 49, 161–171 (1974)
Laskey, R.A., Mills, A.D.: Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. Europ. J. Biochem. 56, 335–341 (1975)
Manuelidis, E.E.: Experiments with tissue culture and heterologous transplantation of tumors. Ann. N.Y. Acad. Sci. 159, 409–431 (1969)
Manuelidis, L.: Repeating restriction fragments of human DNA. Nucleic Acids Res. 3, 3063–3076 (1976)
Manuelidis, L.: A simplified method for preparation of mouse satellite DNA. Anal. Biochem. 78, 561–568 (1977)
Manuelidis, L.: Complex and simple sequences in human repeated DNAs. Chromosoma (Berl.) 66, 1–21 (1978a)
Manuelidis, L.: Chromosomal localization of complex and simple repeated human DNAs. Chromosoma (Berl.) 66, 23–32 (1978b)
Manuelidis, L., Manuelidis, E.E.: The amount of satellite DNA in 4 experimentally induced tumors. Quantitative changes in a glioblastoma producing C-type particles. J. Nat. Cancer Inst. 56, 43–40 (1976b)
Manuelidis, L., Manuelidis, E.E.: Localization of mouse satellite DNA on chromosomes of experimentally induced glioblastomas. Non centromeric label in one glioblastoma producing C-type particles. Int. J. Cancer 17, 659–669 (1976c)
Manuelidis, L., Manuelidis, E.E.: Surface growth characteristics of defined normal and neoplastic neuroectodermal cells in vitro. In: Progress in Neuropathology (H.M. Zimmerman, ed.), vol. 4 (in press, 1979)
Manuelidis, L., Wu, J.: Homology between human and simian repeated DNA. Nature (Lond.) 276, 92–94 (1978)
Manuelidis, L., Yu, R.K. Manuelidis, E.E.: Ganglioside content and pattern in human gliomas in culture. Correlation of morphological changes with altered gangliosides. Acta neuropath. (Berl.) 38, 129–135 (1977)
Mark, J., Levan, G., Mitelman, F.: Identification by fluorescence of the G chromosome lost in human meningomas. Hereditas (Lund) 71, 163–168 (1972)
Mark, J., Ponten, J., Westermark, B.: Cytogenetical studies with G band technique of established cell lines of human malignant gliomas. Hereditas (Lund) 78, 304–307 (1974)
McKay, R.D.G., Bobrow, M., Cooke, H.J.: The identification of a repated DNA sequence involved in the karyotype polymorphism of the human Y chromosome. Cytogenet. Cell Genet. 21, 19–32 (1978)
Mears, J.G., Ramirez, F., Leibowitz, D., Nakamura, F., Bloom, A., Konotey-Ahula, F., Bank, A.: Changes in restricted human cellular DNA fragments containing globin gene sequences in thalassemias and related disorders. Proc. Nat. Acad. Sci. (Wash.) 75, 1222–1226 (1978)
Nichols, E.A., Ruddle, F.A.: A review of enzyme polymorphism linkage and electrophoretic conditions for mouse and somatic cell hybrids in starch gels. J. Histochem. Cytochem. 21, 1066–1081 (1973)
Nowell, P.C.: Cytogenetics, In: Cancer (F.F. Becker, ed.), vol. 1, p. 3–31. New York: Plenum Press 1975
Ohno, S.: Aneuploidy as a possible means employed by malignant cells to express recessive phenotypes, In: Chromosomes and cancer (J. German, ed.), p. 77–94. New York: J. Wiley 1974
Rowley, J.D.: The relationship of chromosomal abnormalities to neoplasia. Advanc. Pathobiol. 4, 67–73 (1976)
Sedat, J., Manuelidis, L.: A direct approach to the structure of eukaryotic chromosomes. Cold Spr. Harb. Symp. quant. Biol. 42, 331–350 (1977)
Shapiro, D., Schrier, B.: Cell cultures of fetal rat brain. Exp. Cell Res. 77, 239–247 (1973)
Wolf, V.: Theodor Boveri and his book “On the problem of origin of malignant tumors”, In: Chromosomes and cancer (J. German, ed.), p. 3–20. New York: J. Wiley 1974
Author information
Authors and Affiliations
Additional information
This paper is dedicated to the late H.S.N. Greene, our inspired teacher
Rights and permissions
About this article
Cite this article
Manuelidis, L., Manuelidis, E.E. Conservation of repeated DNA sequences in aneuploid human tumor cells. Chromosoma 72, 257–269 (1979). https://doi.org/10.1007/BF00331088
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00331088