Summary
The distribution of acetylcholinesterase (E.C. 3.1.1.7.) and other cholinesterases (E.C. 3.1.1.8.) in the fine structure of the myoneural junction of the rat diaphragma was studied, using acetylthiocholine and butyrylthiocholine as substrates. p]Acetylcholinesterase was located in the muscle sarcoplasm closely related to the postsynaptic membrane. Other cholinesterases are observed in the primary and secondary synaptic clefts and between the axon and the teloglial cells.
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Barrnett, R. J.: The fine structural localization of acetylcholinesterase at the myoneural junction. J. Cell Biol. 12, 247 (1962).
Birks, R., H. E. Huxley, and B. J. Katz: The fine structure of the neuromuscular junction of the frog. J. Physiol. (Lond.) 150, 134 (1960).
Physiological and structural changes at the amphibian myoneural junction, in the course of nerve degeneration. J. Physiol. (Lond.) 150, 145 (1960a).
Bloom, F. E., and R. J. Barrnett: Fine structural localization of acetylcholinesterase activity in eel electroplaques. J. Cell Biol. 27, 13A (Abstract) (1965).
Brzin, M., V. M. Tennyson, and P. E. Duffy: Acetylcholinesterase in frog sympathetic and dorsal root ganglia. J. Cell Biol. 31, 215 (1966).
Dixon, M., and E. C. Webb (eds.): Enzymes, sec. ed. London: Longmans 1964.
Eränkö, O., and H. Teräväinen: Distribution of esterases in the myoneural junction of the striated muscle of the rat. J. Histochem. Cytochem. (In press) (1967).
Holmstedt, B., and F. Sjöqvist: Some principles about histochemistry of cholinesterases with special reference to the thiocholine method. Bibl. anat. (Basel) 2, 1 (1961).
Hydén, H.: The neuron. In: The cell, vol. IV, p. 215. New York and London: Academic Press 1960.
Iwayama, T.: Electron microscopic study on cholinesterase at the neuromuscular junction. Okajimas Folia anat. jap. 42, 329 (1966).
Karnovsky, M. J.: The localization of cholinesterase activity in rat cardiac muscle by electron microscopy. J. Cell Biol. 23, 217 (1964).
Koelle, G. B.: Cholinesterases of the central nervous system. J. Neuropath. exp. Neurol. 14, 23 (1955).
A new concept of neurohumoral functions of acetylcholine and acetylcholinesterase. J. Pharm. Pharmacol. 14, 65 (1962).
(Sub. ed.): Cholinesterases and anticholinesterase agents. In: Handbuch der experimentellen Pharmakologie, Bd. IV, S. 187. Berlin-Göttingen-Heidelberg: Springer 1963.
, and J. S. Friedenwald: A histochemical method for localizing cholinesterase activity. Proc. Soc. exp. Biol. (N.Y.) 70, 617 (1949).
Lewis, P. R., and C. C. D. Shute: The distribution of cholinesterase in cholinergic neurons demonstrated with the electron microscope. J. Cell Sci. 1, 381 (1966).
Luft, J. H.: Improvements in epoxy embedding methods. J. biophys. biochem. Cytol. 9, 409 (1961).
Novikoff, A. B., N. Quintana, H. Villaverde, and R. Forschirm: Nucleoside phosphatase and cholinesterase activities in dorsal root ganglia and peripheral nerve. J. Cell Biol. 29, 525 (1966).
Pearse, A. G. E.: Histochemistry. Theoretical and applied. London: Churchill 1960.
Pease, D. C. (Ed.): In: Histological techniques for electron microscope, p. 30. New York and London: Academic Press 1960.
Aldehydes as fixatives for histochemistry. In: Histological techniques for electron microscope, p. 50. New York and London: Academic Press 1964.
Reynolds, E. S.: The use of lead citrate as an electron dense stain in electron microscopy. J. Cell Biol. 17, 208 (1963).
Sabatini, D. D., K. Bensch, and R. J. Barrnett: Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J. Cell Biol. 17, 19 (1963).
Salpeter, M. M.: Electron microscope radioautography as a quantitative tool in enzyme cytochemistry. J. Cell Biol. 32, 379 (1967).
Schlaepfer, W. W., and R. M. Torack: The ultrastructural localization of cholinesterase activity in the sciatic nerve of the rat. J. Histochem. Cytochem. 14, 815 (1966).
Toschi, G.: A biochemical study of brain microsomes. Exp. Cell Res. 16, 232 (1959).
Whittaker, V. P.: The application of sub-cellular fractionation techniques to the study of brain function. Progr. Biophys. molec. Biol. 15, 39 (1965).
Zacks, S. I., and J. M. Blumberg: The histochemical localization of acetylcholinesterase in the fine structure of neuromuscular junctions of mouse and human intercostal muscles. J. Histochem. Cytochem. 9, 317 (1961).
Observations on the fine structure and cytochemistry of mouse and human intercostal neuromuscular junctions. J. Cell Biol. 10, Suppl. 517 (1961a).
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Teräväinen, H. Electron microscopic localization of cholinesterases in the rat myoneural junction. Histochemie 10, 266–271 (1967). https://doi.org/10.1007/BF00304874
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DOI: https://doi.org/10.1007/BF00304874