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The localisation of succinic dehydrogenase in the muscles of Nereis virens and Homarus gammarus

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Summary

The localisation of succinic dehydrogenase and cytochrome oxidase in body muscles of Nereis virens and in tail muscles of Homarus gammarus was studied. Pig heart muscle was used for some comparisons.

Electron microscopic studies on tissue sections generally showed well developed and independent mitochondria in Homarus gammarus. A lower degree of independence characterised the less developed mitochondria of Nereis virens.

Sections were stained with nitro-BT. Light microscopic studies showed a distinct and selective staining of the mitochondria in sections of Homarus gammarus. In addition to the few mitochondria of Nereis virens strings within the cytoplasm became distinctly blue. Electron microscopic studies on Nereis virens showed a higher electron density along the membranes of the vesicular sarcotubular system in incubated than in non-incubated sections.

The fractions obtained on centrifugation of the homogenised tissues were used for combined enzyme studies and electron microscopic investigation. Similarly prepared fractions from the two invertebrates showed a similar electron microscopic appearance. The supernatants obtained by centrifugation at 12,000 g for 10 minutes contained vesicles different from the majority of those in the “mitochondrial” fractions. These supernatants had rather considerable activities of succinate-cytochrome c reductase and of cytochrome c oxidase. The activity of succinate-cytochrome c reductase was most pronounced in the supernatants of Nereis virens and much greater than the cytochrome c oxidase activity in these fractions. The ratio between succinate-cytochrome c reductase activity and cytochrome c oxidase activity in the supernatants of Nereis virens was about three times that in the corresponding fractions of Homarus gammarus.

Manometric studies were performed to get the effect of added succinate on the O2 uptake of the supernatants obtained by centrifugation at 12,000 g for 10 minutes. A distinctly larger increase in oxygen consumption characterised the supernatants of Nereis virens.

The results presented indicate the occurrence of an extra-mitochondrial succinic dehydrogenase in Nereis virens. This conclusion was related to the occurrence of alternative oxidative systems in the muscles of this invertebrate.

The literature dealing with an extra-mitochondrial localisation of succinic dehydrogenase is briefly reviewed as well as the electron microscopic studies concerning transformations between the membrane structures of cells.

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  1. Zoophysiological Institute, University of Lund, Sweden

    Artur G. M. Mattisson

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Mattisson, A.G.M. The localisation of succinic dehydrogenase in the muscles of Nereis virens and Homarus gammarus . Histochemie 5, 97–115 (1965). https://doi.org/10.1007/BF00285502

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