Summary
An analysis has been made of the biogenesis of the highly ordered lattice structure which comprises an integral part of the cell wall of Chlamydomonas reinhardi. By examining mutants showing various wall defects, it has been shown that in some mutants lattice assembly will only occur in the presence of a particular kind of physical structure; in others the composition of the medium influences assembly. The role of a heritable factor in lattice assembly has also been demonstrated; after prolonged vegetative culture certain mutants lose the ability to form lattice, but regain this ability on passing through the diploid sexual phase. An attempt is made to integrate this information to provide a model for the control of assembly and inheritance of the wall.
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References
Beisson, J., Sonneborn, T. M.: Cytoplasmic inheritance of the organisation of the cell cortex in Paramecium aurelia. Proc. nat. Acad. Sci. (Wash.) 53, 275–282 (1965).
Church, R. L., Pfeiffer, S. E., Tanzer, M. L.: Collagen biosynthesis: synthesis and secretion of a high molecular weight collagen precursor (procollagen). Proc. nat. Acad. Sci. (Wash.) 68, 2638–2642 (1971).
Clive, D., Landman, D. E.: Reversion of Bacillus subtilis protoplasts to the bacillary form induced by exogenous cell wall, bacteria and by growth in membrane filters. J. gen. Microbiol. 61, 233–243 (1970).
Davies, D. R.: Electrophoretic analyses of wall glycoproteins in normal and mutant cells. Exp. Cell Res. 73, 512–516 (1972a).
Davies, D. R.: Cell wall organisation in Chlamydomonas reinhardi: the role of extra-nuclear systems. Molec. gen. Genet. 115, 334–348 (1972b).
Davies, D. R., Plaskitt, A.: Genetical and structural analyses of cell-wall formation in Chlamydomonas reinhardi. Genet. Res. 17, 33–43 (1971).
Hyams, J.: Genetical and ultrastructural analysis of cell wall mutants in Chlamydomonas reinhardi. PhD thesis. University of East Anglia 1972.
Hyams, J., Davies, D. R.: The induction and characterisation of cell wall mutants of Chlamydomonas reinhardi. Mutation Res. 14, 381–389 (1972).
Hills, G. J., Gurney-Smith, M., Roberts, K.: Structure, composition and morphogenesis of the cell wall of Chlamydomonas reinhardi. II. Electron microscopy and optical diffraction analysis. J. Ultrastruct. Res. (in press).
Laemmli, J.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (Lond.) 227, 680–685 (1970).
Landman, O. E., Halle, S.: Enzymically and physically induced inheritance changes in Bacillus subtilis. J. molec. Biol. 7, 721–738 (1963).
Roberts, K., Gurney-Smith, M., Hills, G. J.: Structure, composition and morphogenesis of the cell wall of Chlamydomonas reinhardi. I. Ultrastructure and preliminary chemical analysis. J. Ultrastruct. Res. 40, 599–613 (1972).
Sonneborn, T. M.: Gene action in development. Proc. roy. Soc. B 176, 347–366 (1970).
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Communicated by Ch. Auerbach
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Davies, D.R., Lyall, V. The assembly of a highly ordered component of the cell wall: The role of heritable factors and of physical structure. Molec. Gen. Genet. 124, 21–34 (1973). https://doi.org/10.1007/BF00267161
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DOI: https://doi.org/10.1007/BF00267161