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Cell Cycle Progression and Synchronization: An Overview

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Cell-Cycle Synchronization

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2579))

Abstract

The cell cycle is the series of events that take place in a cell that drives it to divide and produce two new daughter cells. Through more than 100 years of efforts by scientists, we now have a much clearer picture of cell cycle progression and its regulation. The typical cell cycle in eukaryotes is composed of the G1, S, G2, and M phases. The M phase is further divided into prophase, prometaphase, metaphase, anaphase, telophase, and cytokinesis. Cell cycle progression is mediated by cyclin-dependent kinases (Cdks) and their regulatory cyclin subunits. However, the driving force of cell cycle progression is growth factor-initiated signaling pathways that controls the activity of various Cdk-cyclin complexes. Most cellular events, including DNA duplication, gene transcription, protein translation, and post-translational modification of proteins, occur in a cell-cycle-dependent manner. To understand these cellular events and their underlying molecular mechanisms, it is desirable to have a population of cells that are traversing the cell cycle synchronously. This can be achieved through a process called cell synchronization. Many methods have been developed to synchronize cells to the various phases of the cell cycle. These methods could be classified into two groups: synchronization methods using chemical inhibitors and synchronization methods without using chemical inhibitors. All these methods have their own merits and shortcomings.

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  1. Department of Medical Genetics, University of Alberta, Edmonton, AB, Canada

    Zhixiang Wang

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    Zhixiang Wang

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Wang, Z. (2022). Cell Cycle Progression and Synchronization: An Overview. In: Wang, Z. (eds) Cell-Cycle Synchronization. Methods in Molecular Biology, vol 2579. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2736-5_1

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  • DOI: https://doi.org/10.1007/978-1-0716-2736-5_1

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2735-8

  • Online ISBN: 978-1-0716-2736-5

  • eBook Packages: Springer Protocols

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