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Non-PCR Amplification Techniques

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Advanced Techniques in Diagnostic Microbiology

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Abstract

Signal, probe, and non-PCR target amplification methods have been developed as viable alternatives to PCR testing for infectious agents. These signal amplification methods include hybrid capture and Invader, as well as target amplification methods such as transcription-mediated amplification, strand displacement amplification, loop-mediated isothermal amplification, and helicase-dependent amplification. The principles of these methods and their commercially available FDA-cleared assays are discussed in this chapter.

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Authors and Affiliations

  1. Keck School of Medicine of the University of Southern California, Los Angeles, CA, USA

    Rosemary C. She

  2. Ascension – St. John Providence, Grosse Pointe, MI, USA

    Ted E. Schutzbank

  3. Roche Molecular Systems, Inc., Pleasanton, CA, USA

    Elizabeth M. Marlowe

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  1. Rosemary C. She
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  2. Ted E. Schutzbank
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  3. Elizabeth M. Marlowe
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Correspondence to Rosemary C. She .

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  1. Departments of Laboratory Medicine and Internal Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, USA

    Yi-Wei Tang

  2. Department of Pathology, Microbiology and Immunology and Medicine, Vanderbilt University Medical Center, Nashville, TN, USA

    Charles W. Stratton

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She, R.C., Schutzbank, T.E., Marlowe, E.M. (2018). Non-PCR Amplification Techniques. In: Tang, YW., Stratton, C. (eds) Advanced Techniques in Diagnostic Microbiology. Springer, Cham. https://doi.org/10.1007/978-3-319-33900-9_17

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