Skip to main content
SpringerLink
Log in

Identification and characterization of international Fusarium head blight screening nurseries of wheat at CIMMYT, Mexico

  • Published:
European Journal of Plant Pathology Aims and scope Submit manuscript

Abstract

Fusarium head blight (FHB) is a major threat to wheat production globally, causing not only yield losses but also food and feed contamination. FHB research began at the International Maize and Wheat Improvement Center (CIMMYT) in the early 1980’s, and since then, large-scale FHB screening has been conducted to identify and incorporate new resistance genes into elite CIMMYT germplasm. Promising lines with good FHB resistance were regularly compiled as a Fusarium Head Blight Screening Nursery (FHBSN) and distributed worldwide. The first FHBSN was assembled in 1985, and the most recent two were the 13th and 14th FHBSN that were released in 2011 and 2012, respectively. Candidate lines for a FHBSN came mainly from different CIMMYT wheat breeding programs and were tested for three consecutive years before being included in an FHBSN. FHBSN screening was conducted under strictly standardized field conditions at El Batán, where CIMMYT headquarters is located, using artificial inoculation of F. graminearum strains, whose aggressiveness and DON chemotypes had been previously identified. FHB index was scored at 31 days after inoculation for all lines and DON concentration was measured only for elite lines in their 2nd and 3rd year of evaluation. Haplotyping is a new tool for genetic characterization of FHBSN entries and helps to identify new resistance sources with novel resistance genes and to better target crosses toward diversifying and/or pyramiding resistance. The 13th FHBSN was taken as an example in this paper to show the procedure and strategy for the development of new FHB resistant lines.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Anderson, J. A. (2007). Marker-assisted selection for Fusarium head blight resistance in wheat. International Journal of Food Microbiology, 119, 51–53.

    Article  PubMed  CAS  Google Scholar 

  • Bai, G. H., & Shaner, G. (2004). Management and resistance in wheat and barley to Fusarium head blight. Annual Review of Phytopathology, 42, 135–161.

    Article  PubMed  CAS  Google Scholar 

  • Bekele, G., Singh, R. P., & Alcala, M. (1988). Results of the first international scab resistance screening nursery (SRSN) 1985–86. Mexico, DF: CIMMYT.

    Google Scholar 

  • Buerstmayr, H., Lemmens, M., Hartl, L., Doldi, L., Steiner, B., Stierschneider, M., & Ruckenbauer, P. (2002). Molecular mapping of QTLs for Fusarium head blight resistance in spring wheat. I. Resistance to fungal spread (type II resistance). Theoretical and Applied Genetics, 104, 84–91.

    Article  PubMed  CAS  Google Scholar 

  • CIMMYT. (2005). Laboratory protocols: CIMMYT Applied Molecular Genetics Laboratory. Third Edition. Mexico, DF: CIMMYT.

    Google Scholar 

  • Cuthbert, P. A., Somers, D. J., & Brulé-Babel, A. (2007). Mapping of Fhb2 on chromosome 6BS: A gene controlling Fusarium head blight field resistance in bread wheat (Triticum aestivum L.). Theoretical and Applied Genetics, 114, 429–437.

    Article  PubMed  CAS  Google Scholar 

  • Duveiller, E., Mezzalama, M., Murakami, J., Lewis, J., & Ban, T. (2008). Global fusarium networking. Cereal Research Communications, 36, 11–19.

    Article  Google Scholar 

  • Gilchrist, L., Rajaram, S., Mujeeb-Kazi, A., van Ginkel, M., Vivar, H., & Pfeiffer, W. (1996). Fusarium scab screening program at CIMMYT. In H. J. Dubin, L. Gilchrist, J. Reeves, & A. McNab (Eds.), Fusarium head scab: Global status and future prospects (pp. 7–12). Mexico DF: CIMMYT.

    Google Scholar 

  • Glen, M., Tommerup, I. C., Bougher, N. L., & O’Brien, P. A. (2001). Specificity, sensitivity and discrimination of primers for PCR-RFLP of larger basidiomycetes and their applicability to identification of ectomycorrhizal fungi in Eucalyptus forests and plantations. Mycological Research, 105, 138–149.

    Article  CAS  Google Scholar 

  • Handa, H., Namiki, N., Xu, D., & Ban, T. (2008). Dissecting of the FHB resistance QTL on the short arm of wheat chromosome 2D using a comparative genomic approach: From QTL to candidate gene. Molecular Breeding, 22, 71–84.

    Article  Google Scholar 

  • He, Z. H., van Ginkel, M., Gilchrist, L., & Rajaram, S. (2000). Progress of China/CIMMYT shuttle breeding and germplasm exchange aimed at combining high yield potential with scab resistance. In Proceedings of the National Fusarium Head Blight Forum (pp. 264–268). Cincinnati, USA.

  • Jiang, G. L., Shi, J. R., & Ward, R. W. (2007). QTL analysis of resistance to Fusarium head blight in the novel wheat germplasm CJ 9306. I. Resistance to fungal spread. Theoretical and Applied Genetics, 116, 3–13.

    Article  PubMed  CAS  Google Scholar 

  • Korzun, V., Roder, M. S., Ganal, M. W., Worland, A. J., & Law, C. N. (1998). Genetic analysis of the dwarfing gene (Rht8) in wheat. Part I. Molecular mapping of Rht8 on the short arm of chromosome 2D of bread wheat (Triticum aestivum L.). Theoretical and Applied Genetics, 96, 1104–1109.

    Article  CAS  Google Scholar 

  • Kumar, S., Stack, R. W., Friesen, T. L., & Faris, J. D. (2007). Identification of a novel fusarium head blight resistance quantitative trait locus on chromosome 7A in tetraploid wheat. Phytopathology, 97, 592–597.

    Article  PubMed  CAS  Google Scholar 

  • Lewis, J. M., Trethowan, R., Ban, T., Duveiller, E., & Ward, R. (2007). Preliminary examination of the influence of grain color in FHB resistance. In Proceedings of the National Fusarium Head Blight Forum (p 193). Kansas City, Missouri.

  • Li, H. P., Wu, A. B., Zhao, C. S., Scholten, O., Loffler, H., & Liao, Y. C. (2005). Development of a generic PCR detection of deoxynivalenol- and nivalenol-chemotypes of Fusarium graminearum. FEMS Microbiology Letters, 243, 505–511.

    Article  PubMed  CAS  Google Scholar 

  • Liu, S., Chalhoub, B., Dolezel, J., Zhang, J. X., Trick, H. N., Gill, B. S., Pumphrey, M. O., & Anderson, J. A. (2008). Toward positional cloning of Fhb1, a major QTL for Fusarium head blight resistance in wheat. Cereal Research Communications, 36, 195–201.

    Article  CAS  Google Scholar 

  • Lu, Q., Lillemo, M., Helge, S., He, X., Shi, J., Ji, F., Dong, Y., & Bjørnstad, Å. (2012). Anther extrusion and plant height are associated with Type I resistance to Fusarium head blight resistance in bread wheat line ‘Shanghai-3/Catbird’. Theoretical and Applied Genetics:in press

  • Mesterhazy, A. (1995). Types and components of resistance to Fusarium head blight of wheat. Plant Breeding, 114, 377–386.

    Article  Google Scholar 

  • Mesterhazy, A., Bartok, T., Mirocha, C. G., & Komoroczy, R. (1999). Nature of wheat resistance to Fusarium head blight and the role of deoxynivalenol for breeding. Plant Breeding, 118, 97–110.

    Article  CAS  Google Scholar 

  • Miller, J. D., Young, J. C., & Sampson, D. R. (1985). Deoxynivalenol and Fusarium Head Blight Resistance in Spring Cereals. Journal of Phytopathology, 113, 359–367.

    Article  CAS  Google Scholar 

  • Nicholson, P., Simpson, D. R., Weston, G., Rezanoor, H. N., Lees, A. K., Parry, D. W., & Joyce, D. (1998). Detection and quantification of Fusarium culmorum and Fusarium graminearum in cereals using PCR assays. Physiological and Molecular Plant Pathology, 53, 17–37.

    Article  CAS  Google Scholar 

  • Otto, C. D., Kianian, S. F., Elias, E. M., Stack, R. W., & Joppa, L. R. (2002). Genetic dissection of a major Fusarium head blight QTL in tetraploid wheat. Plant Molecular Biology, 48, 625–632.

    Article  PubMed  CAS  Google Scholar 

  • Parry, D. W., Jenkinson, P., & Mcleod, L. (1995). Fusarium Ear Blight (Scab) in Small-Grain Cereals - a Review. Plant Pathology, 44, 207–238.

    Article  Google Scholar 

  • Quarta, A., Mita, G., Haidukowski, M., Logrieco, A., Mule, G., & Visconti, A. (2006). Multiplex PCR assay for the identification of nivalenol, 3-and 15-acetyl-deoxynivalenol chemotypes in Fusarium. FEMS Microbiology Letters, 259, 7–13.

    Article  PubMed  CAS  Google Scholar 

  • Quarta, A., Mita, G., Haidukowski, M., Santino, A., Mule, G., & Visconti, A. (2005). Assessment of trichothecene chemotypes of Fusarium culmorum occurring in Europe. Food Additives and Contaminants, 22, 309–315.

    Article  PubMed  CAS  Google Scholar 

  • Schlang, N., & Duveiller, E. (2010). Current approaches and utilization of new screening techniques for evaluation of FHB resistance at CIMMYT. In Proceedings of the 11th European Fusarium Seminar (pp. 87–88). Radzikow, Poland.

  • Schlang, N., Mezzalama, M., Chao, S., Dreisigacker, S., & Duveiller, E. (2009). Results from the Second Fusarium International Spring Wheat Nursery (FIEPSN). In Proceedings of the National Fusarium Head Blight Forum (p 145). Orlando, USA.

  • Schuelke, M. (2000). An economic method for the fluorescent labeling of PCR fragments. Nature Biotechnology, 18, 233–234.

    Article  PubMed  CAS  Google Scholar 

  • Singh, R. P., Ma, H., & Rajaram, S. (1995). Genetic-analysis of resistance to scab in spring wheat cultivar Frontana. Plant Disease, 79, 238–240.

    Article  Google Scholar 

  • Singh, S., Franks, C. D., Huang, L., Brown-Guedira, G. L., Marshall, D. S., Gill, B. S., & Fritz, A. (2004). Lr41, Lr39, and a leaf rust resistance gene from Aegilops cylindrica may be allelic and are located on wheat chromosome 2DS. Theoretical and Applied Genetics, 108, 586–591.

    Article  PubMed  CAS  Google Scholar 

  • Snijders, C. H. A. (1990). The Inheritance of Resistance to Head Blight Caused by Fusarium-Culmorum in Winter-Wheat. Euphytica, 50, 11–18.

    Article  Google Scholar 

  • Somers, D. J., Fedak, G., & Savard, M. (2003). Molecular mapping of novel genes controlling Fusarium head blight resistance and deoxynivalenol accumulation in spring wheat. Genome, 46, 555–564.

    Article  PubMed  CAS  Google Scholar 

  • Stack, R. W., & McMullen, M. P. (1994). A visual scale to estimate severity of Fusarium head blight in wheat. North Dakota State University Extension Service (p 1095).

  • Steiner, B., Lemmens, M., Griesser, M., Scholz, U., Schondelmaier, J., & Buerstmayr, H. (2004). Molecular mapping of resistance to Fusarium head blight in the spring wheat cultivar Frontana. Theoretical and Applied Genetics, 109, 215–224.

    Article  PubMed  CAS  Google Scholar 

  • Summerell, B. A., & Leslie, J. F. (2011). Fifty years of Fusarium: How could nine species have ever been enough? Fungal Diversity, 50, 135–144.

    Article  Google Scholar 

  • van Ginkel, M., & Gilchrist, L. (2002). How to make intelligent crosses to accumulate Fusarium Head Blight resistance genes based on knowledge of the underlying resistance mechanisms. In Proceedings of the National Fusarium Head Blight Forum (pp. 268–272). Cincinnati, USA.

  • van Ginkel, M., Gilchrist, L., Capettini, F., Kazi, M., Pfeiffer, W., William, M., Ban, T., & Lillemo, M. (2003). Breeding for Fusarium head blight resistance: An international approach. In Proceedings of the National Fusarium Head Blight Forum (pp. 289–293). Minneapolis, USA.

  • van Ginkel, M., Gilchrist, L., Capettini, F., Mujeeb-Kazi, A., Pfeiffer, W., Manilal, W., Ban, T., & Lillemo, M. (2004). Breeding for Global Resistance to FHB. In Collaborative Research for Fusarium Head Blight Resistance in Wheat and Barley (pp. 19–24). Tsukuba, Japan.

  • Wisniewska, H., Perkowski, J., & Kaczmarek, Z. (2004). Scab response and deoxynivalenol accumulation in spring wheat kernels of different geographical origins following inoculation with Fusarium culmorum. Journal of Phytopathology, 152, 613–621.

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The helpful assistance of Francisco Lopez and Javier Segura with field trials and Nerida Lozano for her efforts on strain identification and inoculum preparation is highly acknowledged. Editing assistance was received from Emma Quilligan. We are grateful also to Dr. Jiro Murakami for providing the rice medium protocol, and to Sayaka Niwa, Yokohama City University, for her help on characterizing the 2DS QTL in our materials. The authors would also like to thank the collaborative institutions for kindly sharing their FHBSN data.

Author information

Authors and Affiliations

  1. International Maize and Wheat Improvement Center (CIMMYT), Apdo, Postal 6–641, 06600, Mexico, DF, Mexico

    Xinyao He, Pawan K. Singh, Etienne Duveiller, Norbert Schlang, Susanne Dreisigacker & Ravi P. Singh

Authors
  1. Xinyao He
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Pawan K. Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Etienne Duveiller
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Norbert Schlang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Susanne Dreisigacker
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Ravi P. Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Pawan K. Singh.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Table S1

(DOCX 24 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

He, X., Singh, P.K., Duveiller, E. et al. Identification and characterization of international Fusarium head blight screening nurseries of wheat at CIMMYT, Mexico. Eur J Plant Pathol 136, 123–134 (2013). https://doi.org/10.1007/s10658-012-0146-7

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10658-012-0146-7

Keywords

Search

Navigation