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Effects of low-level laser therapy on ROS homeostasis and expression of IGF-1 and TGF-β1 in skeletal muscle during the repair process

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Abstract

The aim of the present study was to determine the effects of low-level laser therapy (LLLT) on the homeostasis of reactive oxygen species (ROS) and expression of IGF-1 and TGF-β1 in the gastrocnemius muscles of rats following contusion. Muscle regeneration involves cell proliferation, migration, and differentiation and is regulated by growth factors. A growing body of evidence suggests that LLLT promotes skeletal muscle regeneration and accelerates tissue repair. Adult male Sprague-Dawley rats (n = 96) were randomly divided into three groups: control group (no lesion, untreated, n = 6), contusion group (n = 48), and contusion-plus-LLLT group (n = 42). Gallium aluminum arsenide (GaAlAs) laser irradiation (635 nm; beam spot, 0.4 cm2; output power, 7 mW; power density, 17.5 mW/cm2; 20 min) was administered to the gastrocnemius contusion for 20 min daily for 10 days. Muscle remodeling was evaluated at 0 h and 1, 2, 3, 7, 14, 21, and 28 days after injury. Hematoxylin and eosin and Van Gieson staining were used to evaluate regeneration and fibrosis; muscle superoxide dismutase (SOD) and malondialdehyde (MDA) were detected via biochemical methods; expression of transforming growth factor beta-1 (TGF-β1) and insulin-like growth factor-1 (IGF-1) were investigated via immunohistochemistry. The results showed that LLLT markedly promoted the regeneration of muscle and reduced scar formation. LLLT also significantly enhanced muscle SOD activity and significantly decreased muscle MDA levels 1, 2, and 3 days after injury. LLLT increased the expression of IGF-1 2, 3, and 7 days after injury and decreased the expression of IGF-1 21 and 28 days after injury. LLLT decreased the expression of TGF-β1 3 and 28 days after injury but increased expression at 7 and 14 days after injury. Our study showed that LLLT could modulate the homeostasis of ROS and of the growth factors IGF-1 and TGF-β1, which are known to play important roles in the repair process. This may constitute a new preventive approach to muscular fibrosis.

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Competing interests

The authors have declared that no competing interests exist.

Funding

This work was supported by the National Science Foundation of China (60878061)

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Authors and Affiliations

  1. School of Physical Education and Sports Science, Soochow University, Suzhou, 215021, China

    Li Luo & Lin Zhang

  2. School of Medicine, Soochow University, Suzhou, 215021, China

    Li Luo

  3. Department of Microbiology and Immunology, Suzhou Health College of Vocational Technology, Suzhou, 215104, China

    Zhongwen Sun

  4. Department of Pathology, Suzhou Health College of Vocational Technology, Suzhou, 215104, China

    Xiaoning Li

  5. Department of Orthopaedics and Sports Medicine, Huashan Hospital, Fudan University, Shanghai, 200040, China

    Yu Dong

  6. College of Sports Science and Research Center of Nationalistic Constitution and Health, South China Normal University, Guangzhou, 510006, China

    Timon Cheng-Yi Liu

  7. Laboratory of Laser Sports Medicine, South China Normal University, University Town, Guangzhou, Guangdong, 510006, China

    Timon Cheng-Yi Liu

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  1. Li Luo
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  3. Lin Zhang
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  4. Xiaoning Li
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  6. Timon Cheng-Yi Liu
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Correspondence to Timon Cheng-Yi Liu.

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Luo, L., Sun, Z., Zhang, L. et al. Effects of low-level laser therapy on ROS homeostasis and expression of IGF-1 and TGF-β1 in skeletal muscle during the repair process. Lasers Med Sci 28, 725–734 (2013). https://doi.org/10.1007/s10103-012-1133-0

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  • DOI: https://doi.org/10.1007/s10103-012-1133-0

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