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HVP10 encoding V-PPase is a prime candidate for the barley HvNax3 sodium exclusion gene: evidence from fine mapping and expression analysis

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Abstract

In cereals, a common salinity tolerance mechanism is to limit accumulation of Na+ in the shoot. In a cross between the barley variety Barque-73 (Hordeum vulgare ssp. vulgare) and the accession CPI-71284 of wild barley (H. vulgare ssp. spontaneum), the HvNax3 locus on chromosome 7H was found to determine a ~10–25 % difference in leaf Na+ accumulation in seedlings grown in saline hydroponics, with the beneficial exclusion trait originating from the wild parent. The Na+ exclusion allele was also associated with a 13–21 % increase in shoot fresh weight. The HvNax3 locus was delimited to a 0.4 cM genetic interval, where it cosegregated with the HVP10 gene for vacuolar H+-pyrophosphatase (V-PPase). Sequencing revealed that the mapping parents encoded identical HVP10 proteins, but salinity-induced mRNA expression of HVP10 was higher in CPI-71284 than in Barque-73, in both roots and shoots. By contrast, the expression of several other genes predicted by comparative mapping to be located in the HvNax3 interval was similar in the two parent lines. Previous work demonstrated roles for V-PPase in ion transport and salinity tolerance. We therefore considered transcription levels of HVP10 to be a possible basis for variation in shoot Na+ accumulation and biomass production controlled by the HvNax3 locus under saline conditions. Potential mechanisms linking HVP10 expression patterns to the observed phenotypes are discussed.

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Abbreviations

CAPS:

Cleaved amplified polymorphic sequence

HVP:

Hordeum vacuolar H+-pyrophosphatase

MFS:

Major facilitator superfamily

q-RT-PCR:

Quantitate reverse transcriptase polymerase chain reaction

ORF:

Open reading frame

SAM:

Sterile alfa motif

V-PPase:

Vacuolar H+-pyrophosphatase

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Acknowledgments

This work was supported by funding to the ACPFG from the ARC, GRDC, and the South Australian government. We gratefully acknowledge Jason Eglinton (Barley Breeding Lab) and Ken Chalmers (Molecular Marker Lab) for the genetic resources used as the basis for this project.

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Authors and Affiliations

  1. Australian Centre for Plant Functional Genomics, School of Agriculture, Food and Wine, University of Adelaide, Urrbrae, SA, 5064, Australia

    Yuri Shavrukov, Jessica Bovill, Irfan Afzal, Julie E. Hayes, Stuart J. Roy, Mark Tester & Nicholas C. Collins

  2. Department of Crop Physiology, University of Agriculture, Faisalabad, 38040, Pakistan

    Irfan Afzal

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  1. Yuri Shavrukov
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  2. Jessica Bovill
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  3. Irfan Afzal
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Correspondence to Yuri Shavrukov.

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Shavrukov, Y., Bovill, J., Afzal, I. et al. HVP10 encoding V-PPase is a prime candidate for the barley HvNax3 sodium exclusion gene: evidence from fine mapping and expression analysis. Planta 237, 1111–1122 (2013). https://doi.org/10.1007/s00425-012-1827-3

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