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Quantitative trait loci for salinity tolerance in barley (Hordeum vulgare L.)

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Abstract

Salinity stress is a major limitation in barley production. Substantial genetic variation in tolerance occurs among genotypes of barley, so the development of salt-tolerant cultivars is a potentially effective approach for minimizing yield losses. The lack of economically viable methods for screening salinity tolerance in the field remains an obstacle to breeders, and molecular marker-assisted selection is a promising alternative. In this study, salinity tolerance of 172 doubled-haploid lines generated from YYXT (salinity-tolerant) and Franklin (salinity-sensitive) was assessed in glasshouse trials during the vegetative phase. A high-density genetic linkage map was constructed from 76 pairs of simple sequence repeats and 782 Diversity Arrays Technology markers which spanned a total of 1,147 cM. Five significant quantitative trait loci (QTL) for salinity tolerance were identified on chromosomes 1H, 2H, 5H, 6H and 7H, accounting for more than 50% of the phenotypic variation. The tolerant variety, YYXT, contributed the tolerance to four of these QTL and Franklin contributed the tolerance to one QTL on chromosome 1H. Some of these QTL mapped to genomic regions previously associated with salt tolerance in barley and other cereals. Markers associated with the major QTL identified in this study have potential application for marker-assisted selection in breeding for enhanced salt tolerance in barley.

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Acknowledgments

This work was supported by a Grains Research and Development Corporation of Australia grant to Meixue Zhou.

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Authors and Affiliations

  1. National Maize Improvement Center of China, China Agricultural University, Beijing, 100193, People’s Republic of China

    Gaofeng Zhou

  2. Tasmanian Institute of Agricultural Research, University of Tasmania, P.O. Box 46, Kings Meadows, TAS, 7249, Australia

    Gaofeng Zhou, Peter Johnson & Meixue Zhou

  3. CSIRO Plant Industry, GPO Box 1600, Canberra, ACT, 2601, Australia

    Gaofeng Zhou, Peter R. Ryan & Emmanuel Delhaize

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  1. Gaofeng Zhou
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  2. Peter Johnson
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Correspondence to Meixue Zhou.

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Zhou, G., Johnson, P., Ryan, P.R. et al. Quantitative trait loci for salinity tolerance in barley (Hordeum vulgare L.). Mol Breeding 29, 427–436 (2012). https://doi.org/10.1007/s11032-011-9559-9

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