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Electrical microenvironment influence on the hydrolytic activity of free and immobilised Yarrowia lipolytica lipase

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Summary

The role of electrical properties of interfaces upon the activity of free and immobilised Yarrowia lipolytica lipase has been investigated. Sodium taurocholate and Sedipur 400, an anionic polyacrylamide, enhance the negative character of the fatty droplets of substrate and tend to improve the lipolytic activity while the cationic polyacrylamide (Sedipur 900) has opposite effects. Ca2+ which reduces the fatty droplets charge as Sedipur 900, is however a good activator of the enzyme. The role of electrical properties on the optimum pH of the immobilised enzyme is clearer. Immobilisation of the lipase on a positively charged support shifts its optimum pH to acidic pH by repulsion towards H+ ions around the support.

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Authors and Affiliations

  1. Gembloux Faculty of Agronomical Sciences, Department of Food Science, 2, Passage des Déportés, 5030, Gembloux, Belgium

    M. Paquot, M. Deleu, C. Beaufils & C. Blecker

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  1. M. Paquot
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  2. M. Deleu
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  3. C. Beaufils
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  4. C. Blecker
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Paquot, M., Deleu, M., Beaufils, C. et al. Electrical microenvironment influence on the hydrolytic activity of free and immobilised Yarrowia lipolytica lipase. Biotechnol Lett 18, 73–78 (1996). https://doi.org/10.1007/BF00137814

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  • DOI: https://doi.org/10.1007/BF00137814

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