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Production of Doubled Haploids in Brassica

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Advances in Haploid Production in Higher Plants

The production of doubled haploid (DH) plants is one of the most important developments in biotechnology. Haploids and DH have been produced in Brassica ssp. using anther culture or isolated microspores. The microspore culture is a simple and affordable technique for production of DH plants, and therefore is the method of choice for plant genetic research and breeding programmes in oilseed Brassicas. Microspore culture is a useful tool in producing doubled homozygous lines for breeding since lines exhibiting desired agronomic traits can be rapidly obtained. Microspore culture is also useful in gene transfer, biochemical and physiological studies and in the production of desired traits such as herbicide resistance and fatty acid modification through mutagenesis and selection

Factors influencing DH production as well as current applications of Brassica doubled haploidy are discussed in this work

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Authors and Affiliations

  1. Instituto de Agricultura Sostenible, CSIC, Córdoba, 14080, Spain

    J. Gil-Humanes & F. Barro

Authors
  1. J. Gil-Humanes
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  2. F. Barro
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Correspondence to F. Barro .

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Editors and Affiliations

  1. Max F. Perutz Laboratories, University of Vienna, Dr Bohrgasse 9/4, Vienna, 1030, Austria

    Alisher Touraev

  2. BioHybrids International Ltd. Earley, Earley, Reading, RG6 5FY, United Kingdom

    Brain P. Forster

  3. Department of Applied BiologyDepartment of Applied Biology, University of Helsinki, Latokartanonkaari 5, Helsinki, FI-00014, Finland

    S. Mohan Jain

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Gil-Humanes, J., Barro, F. (2009). Production of Doubled Haploids in Brassica . In: Touraev, A., Forster, B.P., Jain, S.M. (eds) Advances in Haploid Production in Higher Plants. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-8854-4_4

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