Abstract
Transient gene expression is a rapid and reproducible approach for the production of therapeutics in large scale. Agrobacterium tumefaciens (diarmed tumorigenicity) is employed by introducing recombinant plasmids into plant intracellular spaces in plant cell through syringe or vacuum infiltration that results in ectopic integration into the plant genome. Gene expression usually occurs 3–4 days post-infiltration. Cytoplasmic proteolytic cleavage and posttranscriptional gene silencing (PTGS) are the limiting factors negatively affecting transgene expression. PTGS may be the limiting factor for heterologous protein expression in plant host system. In order to overcome the issues associated with PTGS and other factors that limit protein expression, several strategies are followed. Transient expression studies for high-level expression or modification in viral vectors by incorporating strong promoter or deconstructed virus and viral regulatory elements/co-expression of PTGS suppressor protein are among a few. The advent of modified yield-giving strategies is also regrettably inadequate to meet the expectations; this may be due to cytoplasmic proteolysis of the heterologous protein. Cytoplasmic persistence of transgenic protein may be improved by intracellular compartmentalization. There are many latent shortcomings addressed in this book chapter on strategies to improve overall yield of high-value protein therapeutics in plants.
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The authors thank financial support to Department of Biotechnology, Bharathiar University, under DST- PURSE scheme.
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Iyappan, G., Omosimua, R.O., Sathishkumar, R. (2019). Enhanced Production of Therapeutic Proteins in Plants: Novel Expression Strategies. In: Sathishkumar, R., Kumar, S., Hema, J., Baskar, V. (eds) Advances in Plant Transgenics: Methods and Applications. Springer, Singapore. https://doi.org/10.1007/978-981-13-9624-3_15
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