Skip to main content

Microbial Treatment of Waste by Culture-Dependent and Culture-Independent Approaches: Opportunities and Challenges

  • Chapter
  • First Online:
Microbial Diversity in Ecosystem Sustainability and Biotechnological Applications

Abstract

Globally, there are concerning trends of waste generation as we progress towards a more developed society. India, the second most populous country, is on the edge of being the fifth largest economy in the world. In the last five decades, its metropolitanization has increased by approximately 10% and would further rise to 20% by 2026. The increasing urbanization and evolving lifestyles, food habits, and standards prompt such exponential expansion of robust waste. It is basically answerable for the introduction of a waste era posing various threats to health and causing ecological deformities. In 1996, the urban number created around 114,576 tonnes of municipal solid waste (MSW) per day, which is predicted to be 440,460 tonnes per day−1 by 2026. A large section of the waste is handled using the concept of 3Rs (reduce, reuse, and recycle), but the biological microbial potential is still underestimated. Less than 1% of the microbial community is culturable due to which the conservative cultural microbiology lags behind in revealing the hidden potential. This limitation is subsided by the introduction of culture-independent techniques. This chapter discusses about the possibilities of waste management using microbes both at culture-dependent and culture-independent levels. Next-generation sequencing is a relatively new and flourishing field and promises to be potent enough to supervise the waste generation. Different techniques and procedures have been discussed. The future challenges for waste management lie in the lack of motivation and public unawareness. Waste management is an immediate need of the hour for a sustainable future tomorrow as covered in the Sustainable Development Goals Agenda 2030.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 189.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 249.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Similar content being viewed by others

References

  • Agarwal SK (1998) Advances in environmental biotechnology, 1st edn. APH Publishing Corporation, New Delhi, pp 267–289

    Google Scholar 

  • Ahsan N (1999) Solid waste management plan for Indian megacities. Indian J Environ Prot 19:90–95

    CAS  Google Scholar 

  • Ali U, Syed JH, Malik RN, Katsoyiannis A, Li J et al (2014) Organochlorine pesticides (OCPs) in South Asian region: a review. Sci Total Environ 476:705–717

    Article  PubMed  CAS  Google Scholar 

  • Ambulkar AR, Shekdar AV (2004) Prospects of biomethanation technology in the Indian context: a pragmatic approach. Resour Conserv Recycl 40(2):111–128

    Article  Google Scholar 

  • Angly FE, Felts B, Breitbart M, Salamon P, Edwards RA et al (2006) The marine viromes of four oceanic regions. PLoS Biol 4:e368

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Annepu RK (2012) Report on sustainable solid waste management in India. Waste-to-Energy Research and Technology Council (WTERT) 1–189. See http://swmindia.blogspot.in/. Accessed 26 June 2015

  • Antizar-Ladislao B, Katerina S, Angus JB, Nicholas JR (2008) Microbial community structure changes during bioremediation of PAHs in an aged coal tar contaminated soil by in-vessel composting. Int Biodeterior Biodegradation 61:357–364

    Article  CAS  Google Scholar 

  • Bank W. O. L. D (2012) What a waste – a global review of solid waste management. New York. No 15

    Google Scholar 

  • Batzoglou S, Jaffe DB, Stanley K, Butler J, Gnerre S et al (2002) ARACHNE: a wholegenome shotgun assembler. Genome Res 12:177–189

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Bouchez T, Patureau D, Dabert P, Juretschko S, Doré J et al (2000) Ecological study of a bioaugmentation failure. Environ Microbiol 2:179–190

    Article  CAS  PubMed  Google Scholar 

  • Bouwer EJ, Zehnder AJ (1993) Bioremediation of organic compounds – putting microbial metabolism to work. Trends Biotechnol 11:360–367

    Article  CAS  PubMed  Google Scholar 

  • Bredberg K, Persson J, Christiansson M, Stenberg B, Holst O (2001) Anaerobic desulfurization of ground rubber with the thermophilicarchaeon Pyrococcus furiosus – a new method for rubber recycling. Appl Microbiol Biotechnol 55(1):43–48

    Article  CAS  PubMed  Google Scholar 

  • Central Pollution Control Board (CPCB) (2000a) Management of municipal solid waste Delhi. See http://www.cpcb.nic.in/divisionsofheadoffice/pcp/MSW_Report.pdf. Accessed 27 June 2015

  • Central Pollution Control Board (CPCB) (2000b) Status of municipal solid waste generation, collection, treatment and disposal in class I cities. Series: ADSORBS/31/1999–2000

    Google Scholar 

  • Central Pollution Control Board (CPCB) (2000c) Status of solid waste generation, collection, treatment and disposal in metrocities. Series: CUPS/46/1999–2000

    Google Scholar 

  • Central Pollution Control Board (CPCB) (2004) Management of municipal solid waste. Ministry of Environment and Forests, New Delhi

    Google Scholar 

  • Central Pollution Control Board (CPCB) (2012) Status of compliance by CPCB with municipal solid wastes (management and Handling) rules, 2000

    Google Scholar 

  • Central Pollution Control Board (CPCB) (2013) Status report on municipal solid waste management

    Google Scholar 

  • Chen WM, Wu CH, James EK, Chang JS (2008) Metal biosorption capability of Cupriavidus taiwanensis and its effects on heavy metal removal by nodulated Mimosa pudica. J Hazard Mater 151(2–3):364–371

    Article  CAS  PubMed  Google Scholar 

  • Dayal G (1994) Solid wastes: sources, implications and management. Indian J Environ Prot 14(9):669–677

    Google Scholar 

  • Enright AJ, Iliopoulos I, Kyrpides NC, Ouzounis CA (1999) Protein interaction maps for complete genomes based on gene fusion events. Nature 402:86–90

    Article  CAS  PubMed  Google Scholar 

  • Fierer N, Bradford MA, Jackson RB (2007) Toward an ecological classification of soil bacteria. Ecology 88(6):1354–1364

    Article  PubMed  Google Scholar 

  • Fierer N, Leff JW, Adams BJ, Nielsen UN, Bates ST et al (2012) Cross-biome metagenomic analyses of soil microbial communities and their functional attributes. Proc Natl Acad Sci U S A 109(52):21390–21395

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Finn RD, Tate J, Mistry J, Coggill PC, Sammut SJ et al (2008) The Pfam protein families database. Nucleic Acids Res 36:D281–D288

    Article  CAS  PubMed  Google Scholar 

  • Gandolla M, Aragno M (1992) The importance of microbiology in waste management. Experientia 48:362

    Article  Google Scholar 

  • Garg P, Gupta A, Satya S (2006) Vermicomposting of different types of waste using Eisenia foetida: a comparative study. Bioresour Technol 97:391–395

    Article  CAS  PubMed  Google Scholar 

  • Ghosh P, Swati, Thakur IS (2014) Enhanced removal of COD and color from landfill leachate in a sequential bioreactor. Bioresour Technol 170:10–19

    Article  CAS  PubMed  Google Scholar 

  • Gielkens MM, Visser J, de Graaff LH (1997) Arabinoxylan degradation by fungi: characterization of the arabinoxylan-arabinofuranohydrolase encoding genes from Aspergillus niger and Aspergillus tubingensis. Curr Genet 31(1):22–29

    Article  CAS  PubMed  Google Scholar 

  • Goldfarb KC, Karaoz U, Hanson CA, Santee CA, Bradford MA et al (2011) Differential growth responses of soil bacterial taxa to carbon substrates of varying chemical recalcitrance. Front Microbiol 2:94

    Article  PubMed  PubMed Central  Google Scholar 

  • Gómez G, Meneses M, Ballinas L, Castells F (2009) Seasonal characterization of municipal solid waste (MSW) in the city of Chihuahua, Mexico. Waste Manag 29:2018–2024

    Article  PubMed  Google Scholar 

  • Gordon DC, Abajian C, Green P (1998) Consed: a graphical tool for sequence finishing. Genome Res 8:195–202

    Article  CAS  PubMed  Google Scholar 

  • Gupta R, Mohapatra H (2003) Microbial biomass: an economical alternative for removal of heavy metals from waste water. Indian J Exp Biol 41:945–966

    CAS  PubMed  Google Scholar 

  • Gupta A, Thakur IS (2016) Study of optimization of wastewater contaminant removal along with extracellular polymeric substances (EPS) production by a thermotolerant Bacillus sp. ISTVK1 isolated from heat shocked sewage sludge. Bioresour Technol 213:21–30

    Article  CAS  PubMed  Google Scholar 

  • Gupta J, Rathour R, Kumar M, Thakur IS (2017) Metagenomic analysis of microbial diversity in landfill lysimeter soil of Ghazipur landfill site, New Delhi, India. Genome Announc 5:e01104–e01117

    Article  PubMed  PubMed Central  Google Scholar 

  • Hallam SJ, Konstantinidis KT, Putnam N, Schleper C, Watanabe Y et al (2006) Genomic analysis of the uncultivated marine crenarchaeote Cenarchaeum symbiosum. Proc Natl Acad Sci U S A 103:18296–18301

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Huson DH, Auch AF, Qi J, Schuster SC (2007) MEGAN analysis of metagenomic data. Genome Res 17:377–386

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Indo-UK Seminar Report (2015) Sustainable solid waste management for cities: opportunities in SAARC countries. See http://www.neeri.res.in/Short%20Report_Indo-UK%20Seminar%20(25-27th%20March%202015.pdf. Accessed 27 June 2015

  • Intergovernmental Panel on Climate Change (IPPC) (2007) Climate change 2007: synthesis report. UNEP, WMO: 2007. http://www.ipcc.ch/pdf/assessmentreport/ar4/syr/ar4_syr.pdf. Accessed 20 Dec 2016

  • ISWA (International Solid Waste Association) (2012) Globalization and waste management final report from the ISWA task force. See http://www.iswa.org/knowledgebase/tfgfinal. Accessed 27 Apr 2018

  • Jaffe DB, Butler J, Gnerre S, Mauceli E, Lindblad-Toh K et al (2003) Whole-genome sequence assembly for mammalian genomes: Arachne 2. Genome Res 13:1–96

    Article  CAS  Google Scholar 

  • Jayashree R, Nithya SE, Rajesh PP, Krishnaraju M (2012) Biodegradation capability of bacterial species isolated from oil contaminated soil. J Academia Indust Res 1:140–143

    Google Scholar 

  • Joshi R, Ahmed S (2016) Status and challenges of municipal solid waste management in India: a review. Cogent Environ Sci 2(1):1139434

    Article  Google Scholar 

  • Kansal A (2002) Solid waste management strategies for India. Indian J Environ Prot 22(4):444–448

    Google Scholar 

  • Karigar CS, Rao SS (2011) Role of microbial enzymes in the bioremediation of pollutants: a review. Enzyme Res 2011:1–11

    Article  CAS  Google Scholar 

  • Kaushal RK, Varghese GK, Chabukdhara M (2012) Municipal solid waste management in India-current state and future challenges: a review. Int J Eng Sci Technol 4(4):1473–1489

    Google Scholar 

  • Keegan KP, Glass EM, Meyer F (2016) MG-RAST, a metagenomics service for analysis of microbial community structure and function. Methods Mol Biol 1399:207–233

    Article  CAS  PubMed  Google Scholar 

  • Khan MA, Burney FA (1989) Forecasting solid waste composition – an important consideration in resource recovery and recycling. Resour Conserv Recycl 3(1):1–17

    Article  Google Scholar 

  • Khosla K, Rathour R, Maurya R, Maheshwari N, Gnansounou E et al (2017) Biodiesel production from lipid of carbon dioxide sequestrating bacterium and lipase of psychrotolerant Pseudomonas sp. ISTPL3 immobilized on biochar. Bioresour Technol 245:743–750

    Article  CAS  PubMed  Google Scholar 

  • Kumar JS (2011) Prediction of municipal solid waste with RBF net work – a case study of Eluru, A.P, India. Int J Innov Manag Technol 2:3

    Google Scholar 

  • Kumar KN, Goel S (2009) Characterization of municipal solid waste (MSW) and a proposed management plan for Kharagpur, West Bengal, India. Resour Conserv Recycl 53(3):166–174

    Article  Google Scholar 

  • Kumar A, Samadder SR (2017) A review on technological options of waste to energy for effective management of municipal solid waste. Waste Manag 69:407–422

    Article  CAS  PubMed  Google Scholar 

  • Kumar S, Bhattacharyya JK, Vaidya AN, Chakrabarti T, Devotta S et al (2009) Assessment of the status of municipal solid waste management in metro cities, state capitals, class I cities, and class II towns in India: An insight. Waste Manag 29(2):883–895

    Article  PubMed  Google Scholar 

  • Kumar M, Ghosh P, Khosla K, Thakur IS (2016) Biodiesel production from municipal secondary sludge. Bioresour Technol 216:165–171. https://doi.org/10.1016/j.biortech.2016.05.078

    Article  CAS  PubMed  Google Scholar 

  • Kumar S, Smith SR, Fowler G, Velis C, Kumar SJ et al (2017) Challenges and opportunities associated with waste management in India. R Soc Open Sci 4(3):160764

    Article  PubMed  PubMed Central  Google Scholar 

  • Kumari M, Ghosh P, Wanwari S, Thakur IS (2014) Microcosmic study of endosulfan degradation by Paenibacillus sp. ISTP10 and its toxicological evaluation using mammalian cell line. Int Biodeterior Biodegradation 96:33–40

    Article  CAS  Google Scholar 

  • Kumari M, Ghosh P, Thakur IS (2016) Landfill leachate treatment using bacto-algal co-culture: an integrated approach using chemical analyses and toxicological assessment. Ecotoxicol Environ Saf 128:44–51

    Article  CAS  PubMed  Google Scholar 

  • Kumari M, Ghosh P, Thakur IS (2017) Application of microbes in remediation of hazardous wastes: a review bioremediation: applications for environmental protection and management. Springer, Singapore, pp 223–241

    Google Scholar 

  • Kunin V, Copeland A, Lapidus A, Mavromatis K, Hugenholtz P (2008) A bioinformatician’s guide to metagenomics. Microbiol Mol Biol Rev 72(4):557–578

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Lombardi L, Carnevale E, Corti A (2015) A review of technologies and performances of thermal treatment systems for energy recovery from waste. Waste Manag 37:26–44

    Article  PubMed  Google Scholar 

  • Makan A, Assobhei O, Mountadar M (2013) Effect of initial moisture content on the in-vessel composting under air pressure of organic fraction of municipal solid waste in Morocco. Iran J Environ Health Sci Eng 10(1):3

    Article  Google Scholar 

  • Marcotte EM, Pellegrini M, Ng HL, Rice DW, Yeates TO et al (1999a) Detecting protein function and protein-protein interactions from genome sequences. Science 285:751–753

    Article  CAS  PubMed  Google Scholar 

  • Marcotte EM, Pellegrini M, Thompson MJ, Yeates TO, Eisenberg D (1999b) A combined algorithm for genome-wide prediction of protein function. Nature 402:83–86

    Article  CAS  PubMed  Google Scholar 

  • Margulies M, Egholm M, Altman WE, Attiya S, Bader JS et al (2005) Genome sequencing in microfabricated high-density picolitre reactors. Nature 437:376–380

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Markowitz VM, Ivanova N, Palaniappan K, Szeto E, Korzeniewski F et al (2006) An experimental metagenome data management and analysis system. Bioinformatics 22:e359–e3567

    Article  CAS  PubMed  Google Scholar 

  • Mathur V (2012) Scope of recycling municipal solid waste in Delhi and NCR- integral review. J Manag 5:27–36

    Google Scholar 

  • Medhi K, Thakur IS (2018) Bioremoval of nutrients from wastewater by a denitrifier Paracoccus denitrificans ISTOD1. Bioresour Technol Rep 1:56–60

    Article  Google Scholar 

  • Medhi K, Mishra A, Thakur IS (2018) Genome sequence of a heterotrophic nitrifier and aerobic denitrifier, Paracoccus denitrificans strain ISTOD1, isolated from wastewater. Microbiol Resour Announc 6:15

    Google Scholar 

  • Menzel P et al (2016) Fast and sensitive taxonomic classification for metagenomics with Kaiju. Nat Commun 7:11257

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Mishra A, Jha G, Thakur IS (2018a) Draft genome sequence of Zhihengliuella sp. Strain ISTPL4, a psychrotolerant and halotolerant bacterium isolated from Pangong Lake, India. Genome Announc 6(5):e01533–e01517

    Article  PubMed  PubMed Central  Google Scholar 

  • Mishra A, Medhi K, Maheshwari N, Srivastava S, Thakur IS (2018b) Biofuel production and phycoremediation by Chlorella sp. ISTLA1 isolated from landfill site. Bioresour Technol 253:121–129

    Article  CAS  PubMed  Google Scholar 

  • Modak P, Jiemian Y, Hongyuan Y, Mohanty CR (2010) Municipal solid waste management: turning waste into resources. In: Shanghai manual: a guide for sustainable urban development in the 21st century. Dong fang chu ban zhong xin, Shanghai Shi, pp 1–36

    Google Scholar 

  • Myers EW, Sutton GG, Delcher AL, Dew IM, Fasulo DP et al (2000) A whole-genome assembly of Drosophila. Science 287:2196–2204

    Article  CAS  PubMed  Google Scholar 

  • Nandan A, Yadav BP, Baksi S, Bose D (2017) Recent scenario of solid waste management in India. World Sci News 66:56–74

    CAS  Google Scholar 

  • Niu GL, Zhang JJ, Zhao S, Liu H, Boon N et al (2009) Bioaugmentation of a 4-chloronitrobenzene contaminated soil with Pseudomonas putida ZWL73. Environ Pollut 157:763–771

    Article  CAS  PubMed  Google Scholar 

  • Pappu A, Saxena M, Asolekar SR (2007) Solid wastes generation in India and their recycling potential in building materials. Build Environ 42(6):2311–2320

    Article  Google Scholar 

  • Phelps HO, Heinke GW, Jonker JF, Ouano EAR, Vandecasteele C (1995) Management of solid waste. UNESCO, Paris

    Google Scholar 

  • Planning Commission, Government of India (2014) Report of the task force on waste to energy (Volume I) in the context of integrated municipal solid waste management. See http://planningcommission.nic.in/reports/genrep/rep_wte1205.pdf. Accessed 1 July 2015

  • Qasim SR, Stinehelfer ML (1982) Effect of a bacterial culture product on biological kinetics. J Water Pollut Control Fed 54(3):255

    CAS  Google Scholar 

  • Rathour R, Gupta J, Kumar M, Hiloidhari M, Mehrotra AK et al (2017) Metagenomic sequencing of microbial communities from brackish water of Pangong Lake of the Northwest Indian Himalayas. Genome Announc 5(40):e01029–e01017

    Article  PubMed  PubMed Central  Google Scholar 

  • Schloss PD, Handelsman J (2003) Biotechnological prospects from metagenomics. Curr Opin Biotechnol 14(3):303–310

    Article  CAS  PubMed  Google Scholar 

  • Selengut JD, Haft DH, Davidsen T, Ganapathy A, Gwinn-Giglio M et al (2007) TIGRFAMs and genome properties: tools for the assignment of molecular function and biological process in prokaryotic genomes. Nucleic Acids Res 35:D260–D264

    Article  CAS  PubMed  Google Scholar 

  • Sethunathan N, Megharaj M, Chen ZL, Williams BD, Lewis G et al (2004) Algal degradation of a known endocrine disrupting insecticide, α-endosulfan, and its metabolite, endosulfansulfate, in liquid medium and soil. J Agric Food Chem 52:3030–3035

    Article  CAS  PubMed  Google Scholar 

  • Sharholy M, Ahmad K, Mahmood G, Trivedi RC (2005) Analysis of municipal solid waste management systems in Delhi–a review. In: Book of proceedings for the second international congress of chemistry and environment, Indore, India, pp 773–777

    Google Scholar 

  • Sharholy M, Ahmad K, Vaishya RC, Gupta RD (2007) Municipal solid waste characteristics and management in Allahabad, India. Waste Manag 27(4):490–496

    Article  PubMed  Google Scholar 

  • Sharholy M, Ahmad K, Mahmood G, Trivedi RC (2008) Municipal solid waste management in Indian cities–a review. Waste Manag 28(2):459–467

    Article  PubMed  Google Scholar 

  • Sharma JK, Gautam RK, Nanekar SV, Weber R, Singh BK et al (2017) Advances and erspective in bioremediation of polychlorinated biphenyl-contaminated soils. Environ Sci Pollut Res 25(17):16355–16375

    Article  CAS  Google Scholar 

  • Singh SK, Singh RS (1998) A study on municipal solid waste and its management practices in Dhanbad-Jharia coalfield. Indian J Environ Prot 18(11):850–852

    CAS  Google Scholar 

  • Singh SP, Tiwari G (2014) Application of bioremediation on solid waste management: a review. J Bioremed Biodegr 5:06

    Article  CAS  Google Scholar 

  • Singh R, Singh P, Sharma R (2014) Microorganism as a tool of bioremediation technology for cleaning environment: a review. Proc Int Acad Ecol Environ Sci 4:1–6

    Google Scholar 

  • Singhal L, Tuli AK, Gautam V (2017) Biomedical waste management guidelines 2016: what’s done and what needs to be done. Indian J Microbol 35(2):194

    Google Scholar 

  • Sjostrom E (1993) Wood chemistry, fundamentals and applications, 2nd edn. Gulf Professional Publishing Houston, Texas, p 293

    Google Scholar 

  • Srivastava R, Krishna V, Sonkar I (2014) Characterization and management of municipal solid waste: a case study of Varanasi city. India Int J Curr Res Acad Rev 2:10–16

    CAS  Google Scholar 

  • Stamps BW, Lyles CN, Suflita JM, Masoner JR, Cozzarelli IM et al (2016) Municipal solid waste landfills harbor distinct microbiomes. Front Microbiol 7:534

    Article  PubMed  PubMed Central  Google Scholar 

  • Stephenson D, Stephenson T (1992) Bioaugmentation for enhancing biological wastewater treatment. Biotechnol Adv 10:549–559

    Article  CAS  PubMed  Google Scholar 

  • Swati, Thakur IS, Vijay VK, Ghosh P (2018) Scenario of landfilling in India: problems, challenges, and recommendations. In: Hussain C (ed) Handbook of environmental materials management. Springer, Cham, pp 1–16

    Google Scholar 

  • Tatusov RL, Koonin EV, Lipman DJ (1997) A genomic perspective on protein families. Science 278:631–637

    Article  CAS  PubMed  Google Scholar 

  • Tchobanoglous G, Eliassen R, Theisen H (1977) Solid wastes; engineering principles and management issues. McGraw-Hill, New York

    Google Scholar 

  • Thomas CA Jr (1971) The genetic organization of chromosomes. Annu Rev Genet 5:237–256

    Article  CAS  PubMed  Google Scholar 

  • Tiwari G, Singh SP (2016) Application of bioremediation on solid waste management: a review. In: Solid waste management: policy and planning for a sustainable society, p 143

    Google Scholar 

  • Tuomela M, Vikman M, Hatakka A, Itavaara M (2000) Biodegradation of lignin in a compost environment: a review. Bioresour Technol 72:169–183

    Article  CAS  Google Scholar 

  • UNEP (United Nations Environment Programme) (2005) Solid waste management volume I: http://www.unep.org/ietc/Portals/136/SWM-Vol1-Part1-Chapters1to3.pdf. Accessed 1 July 2015

  • Venter JC, Remington K, Heidelberg JF, Halpern AL, Rusch D et al (2004) Environmental genome shotgun sequencing of the Sargasso Sea. Science 304:66–74

    Article  CAS  PubMed  Google Scholar 

  • Wobus A, Bleul C, Maassen S, Scheerer C, Schuppler M et al (2003) Microbial diversity and functional characterization of sediments from reservoirs of different trophic state. FEMS Microbiol Ecol 46(3):331–347

    Article  CAS  PubMed  Google Scholar 

  • World Bank (2014) World development indicators 2014. World Development Indicators, Washington, DC. © World Bank. https://openknowledge.worldbank.org/handle/10986/18237. License: CC BY 3.0 IGO

  • Zhang H, Hu C, Jia X, Xu Y, Wu C et al (2012) Characteristics of c-hexachlorocyclohexane biodegradation by a nitrogen-fixing cyanobacterium Anabaena azotica. J Appl Phycol 24:221–225

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Indu Shekhar Thakur .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2019 Springer Nature Singapore Pte Ltd.

About this chapter

Check for updates. Verify currency and authenticity via CrossMark

Cite this chapter

Gupta, J., Tyagi, B., Rathour, R., Thakur, I.S. (2019). Microbial Treatment of Waste by Culture-Dependent and Culture-Independent Approaches: Opportunities and Challenges. In: Satyanarayana, T., Johri, B., Das, S. (eds) Microbial Diversity in Ecosystem Sustainability and Biotechnological Applications. Springer, Singapore. https://doi.org/10.1007/978-981-13-8315-1_14

Download citation

Publish with us

Policies and ethics