Skip to main content

A to Z on Banana Micropropagation and Field Practices

  • Chapter
  • First Online:
Plant Tissue Culture: Propagation, Conservation and Crop Improvement

Abstract

In order to stay competitive in the global banana production, it is important to ensure that the planting materials used have consistent superior agronomic traits and are disease-free and grown in farms with good agricultural practice. The use of suckers from field-grown plants as planting materials may increase the risk in the spread of diseases and inconsistent quality. Hence, the adoption of plant tissue culture technique for mass propagation of banana planting materials has been widely used. Micropropagation of bananas has been successfully established through the use of shoot or meristem cultures and inflorescence either through direct plant regeneration or establishment of regenerable cell suspension cultures. From the cell suspension cultures, single-celled protoplasts have also been isolated and regenerated. Not only the in vitro systems developed through tissue culture provide efficient plant production but also a platform for genetic engineering for agronomic traits improvement. In this chapter, we highlight studies on banana micropropagation and field practices of this important crop.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 259.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 329.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 329.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  • Abdallah NA, Shah D, Abbas D, Madkour M (2010) Stable integration and expression of a plant defensin in tomato confers resistance to Fusarium wilt. GM Crops 1:344–350

    Article  PubMed  Google Scholar 

  • Ahirwar MK, Mondal S, Singh MK, Sen C, Singh RP (2012) A high frequency plantlets regeneration protocol for banana (Musa paradisiaca L.) micropropagation. Asian J Hort 7(2):397–401

    Google Scholar 

  • Aoyagi H (2011) Application of plant protoplasts for the production of useful metabolites. Biochem Eng J 56:1–8

    Article  CAS  Google Scholar 

  • Arvanitoyannis IS, Mavromatis A (2009) Banana cultivars, cultivation practices, and physicochemical properties. Crit Rev Food Sci Nutr 49:113–135

    Article  CAS  PubMed  Google Scholar 

  • Arvanitoyannis IS, Mavromatis AG, Grammatikaki-Avgeli G, Sakellariou M (2008) Banana: cultivars, biotechnological approaches and genetic transformation. Int J Food Sci Technol 43:1871–1879

    Article  CAS  Google Scholar 

  • Assani A, Haı¨cour R, Wenzel G, Côte FX, Bakry F, Foroughi-Wehr B, Ducreux G, Aguillar ME, Grapin A (2001) Plant regeneration from protoplasts of dessert banana cv. Grande Naine (Musa spp., Cavendish sub-group AAA) via somatic embryogenesis. Plant Cell Rep 20:482–488

    Article  CAS  Google Scholar 

  • Assani A, Haïcour R, Wenzel G, Foroughi-Wehr B, Bakry F, Côte FX, Ducreux G, Ambroise A, Grapin A (2002) Influence of donor material and genotype on protoplast regeneration in banana and plantain cultivars (Musa spp.). Plant Sci 162:355–362

    Article  CAS  Google Scholar 

  • Assani A, Chabane D, Foroughi-Wehr B, Wenzel G (2006) An improved protocol for microcallus production and whole plant regeneration from recalcitrant banana protoplasts (Musa spp.). Plant Cell Tiss Organ Cult 85:257–264

    Article  Google Scholar 

  • Bakry F (1984) Choix du matériel à utiliser pour I’isolement de protoplastes de bananier (Musa spp.). Friuts 39:449–452

    Google Scholar 

  • Bengochea T, Dodds JH (1986) Plant protoplast: a biotechnological tool for plant improvement. Chapman and Hall Ltd, New York, pp 3–27

    Book  Google Scholar 

  • Beshir I, Sharbasy SE, Safwat G, Diab A (2012) The effect of some natural materials in the development of shoot and root of banana (Musa spp.) using tissue culture technology. New York Sci J 5(1):132–138

    Google Scholar 

  • Cavero PAS, Hanada RE, Gasparotto L, Neto RAC, de Souza JT (2015) Biological control of banana black Sigatoka disease with Trichoderma. Cienc Rural 45(6):951–957

    Article  Google Scholar 

  • Chin WYW, Annuar MSM, Tan BC, Khalid N (2014) Evaluation of a laboratory scale conventional shake flask and a bioreactor on cell growth and regeneration of banana cell suspension cultures. Sci Hortic 172:39–46

    Article  CAS  Google Scholar 

  • Chen WH, Ku ZC (1985) Isolation of mesophyll cells and protoplasts and protoplast fusion and culture in banana. J Agric Assoc China 129:56–67

    CAS  Google Scholar 

  • Čížková J, Hřibová E, Christelová P, Van den Houwe I, Häkkinen M, Roux N, Swennen R, Doležel J (2015) Molecular and cytogenetic characterization of wild Musa species. PLoS One 10:e0134096

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Cronauer S, Krikorian AD (1983) Somatic embryos from cultured tissue of triploid plantains (Musa ABB). Plant Cell Rep 2:289–291

    Article  CAS  PubMed  Google Scholar 

  • Dai XM, Xiao W, Huang X, Zhao JT, Chen YF, Huang XL (2010) Plant regeneration from embryogenic cell suspensions and protoplasts of dessert banana cv. ‘Da Jiao’ (Musa paradisiacal ABB Linn.) via somatic embryogenesis. In Vitro Cell Dev Biol Plant 46:403–410

    Article  Google Scholar 

  • Darvari FD, Sariah M, Puad MP, Maziah M (2010) Micropropagation of some Malaysian banana and plantain (Musa sp.) cultivars using male flowers. Afr J Biotechnol 9(16):2360–2366

    CAS  Google Scholar 

  • Dhanalakshmi S, Stephan R (2014) Low cost media options for the production of banana (Musa paradisiaca L.) through plant tissue culture. J Acad Indus Res 2(9):509–512

    Google Scholar 

  • Divakaran SP, Nair AS (2011) Somatic embryogenesis from bract cultures in diploid Musa acuminata cultivars from South India. Sci Hortic 131:99–102

    Article  Google Scholar 

  • Dong Y, Gao WY, Man S, Zuo B, Wang J, Huang L, Xiao P (2013) Effect of bioreactor angle and aeration rate on growth and hydromechanics parameters in bioreactor culture of ginseng suspension cells. Acta Physiol Plant 35:1497–1501

    Article  CAS  Google Scholar 

  • Eeckhaut T, Lakshmanan PS, Deryckere D, Van Bockstaele E, Van Huylenbroeck J (2013) Progress in plant protoplast research. Planta 238:991–1003

    Article  CAS  PubMed  Google Scholar 

  • Endah R, Beyene G, Kiggundu A, Van den Berg N, Schluter U, Kunert K, Chikwamba R (2008) Elicitor and Fusarium-induced expression of NPR1-like genes in banana. Plant Physiol Biochem 46:1007–1014

    Article  CAS  PubMed  Google Scholar 

  • Epple P, Apel K, Bohlmann H (1997) Overexpression of an endogenous thionin enhances resistance of Arabidopsis against Fusarium oxysporum. Plant Cell 9:509–520

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Escalant JV, Teisson C (1989) Somatic embryogenesis and plants from immature zygotic embryos of species Musa acuminata and Musa balbisiana. Plant Cell Rep 7:665–668

    CAS  PubMed  Google Scholar 

  • Food and Agriculture Organization of the United Nations (FAOSTAT) (2015) Home page at: http://faostat.fao.org/. Accessed 4 Dec 2015

  • George EF, Hall MA, De Klerk GJ (2008) Plant propagation by tissue culture, 3rd edn, vol 1. The background. Springer, Dordrecht

    Google Scholar 

  • Ghag SB, Shekhawat UKS, Ganapathi TR (2014) Transgenic banana plants expressing a Stellaria media defensin gene (Sm-AMP-D1) demonstrate improved resistance to Fusarium oxysporum. Plant Cell Tiss Organ Cult 119:247–255

    Article  CAS  Google Scholar 

  • Ghag SB, Shekhawat UKS, Ganapathi TR (2015) Fusarium wilt of banana: biology, epidemiology and management. Int J Pest Manage 61:250–263

    Article  Google Scholar 

  • Ghosh A, Ganapathi TR, Nath P, Bapat VA (2009) Establishment of embryogenic cell suspension cultures and Agrobacterium-mediated transformation in an important Cavendish banana cv. Robusta (AAA). Plant Cell Tiss Organ Cult 97:131–139

    Article  Google Scholar 

  • Grapin A, Schwendiman J, Teisson C (1996) Somatic embryogenesis in plantain banana. In Vitro Cell Dev Biol Plant 32:66–71

    Article  Google Scholar 

  • Grapin A, Ortíz JL, Lescot T, Ferrière N, Côte FX (2000) Recovery and regeneration of embryogenic cultures from female flowers of False Horn Plantain. Plant Cell Tiss Organ Cult 61:237–244

    Article  CAS  Google Scholar 

  • Haïcour R, Assani A, Trang VB, Guedira A (2009) Protoplast isolation and culture for banana regeneration via somatic embryogenesis. Fruits 64:261–269

    Article  CAS  Google Scholar 

  • Harirah AA, Khalid N (2006) Direct regeneration and RAPD assessment of male inflorescence derived plants of Musa acuminata cv. Berangan. Asia Pacific J Mol Biol Biotechnol 14:11–17

    Google Scholar 

  • Heslop-Harrison JS, Schwarzacher T (2007) Domestication, genomics and the future for banana. Annals Bot 100:1073–1084

    Article  CAS  Google Scholar 

  • Hrahsel L, Basu A, Sahoo L, Thanqiam R (2014) In vitro propagation and assessment of the genetic fidelity of Musa acuminata (AAA) cv. vaibalhla derived from immature male flowers. Appl Biochem Biotechnol 172(3):1530–1539

    Article  CAS  PubMed  Google Scholar 

  • Hui AV, Bhatt A, Keng CL (2012) Micropropagation of Musa acuminata × M. balbisiana cv. Pisang awak (ABB genome) and three other cultivars. Pakistan J Bot 44(2):777–780

    Google Scholar 

  • Husin N, Jalil M, Othman RY, Khalid N (2014) Enhancement of regeneration efficiency in banana (Musa acuminata cv. Berangan) by using proline and glutamine. Sci Hortic 168:33–37

    Article  CAS  Google Scholar 

  • Iqbal MM, Muhammad A, Iqbal H, Bilal H (2013) Optimization of in vitro micropropagation protocol for banana (Musa sapientum L.) under different hormonal concentrations and growth media. Int J Agric Innov Res 2(1):23–27

    Google Scholar 

  • Jafari N, Othman RY, Khalid N (2011) Effect of benzylaminopurine (BAP) pulsing on in vitro shoot multiplication of Musa acuminata (banana) cv. Berangan Afr J Biotechnol 10(13):2446–2450

    CAS  Google Scholar 

  • Jafari N, Othman RY, Tan BC, Khalid N (2015) Morphohistological and molecular profiles during the developmental stages of somatic embryogenesis of Musa acuminata cv. ‘Berangan’ (AAA). Acta Physiol Plant 37:45

    Article  CAS  Google Scholar 

  • Jalil M, Khalid N, Othman RY (2003) Plant regeneration from embryogenic suspension cultures of Musa acuminata cv. Mas (AA). Plant Cell Tiss Organ Cult 75:209–214

    Article  CAS  Google Scholar 

  • Jalil M, Chee WW, Othman RY, Khalid N (2008) Morphohistological examination on somatic embryogenesis of Musa acuminata cv. Mas (AA). Sci Hortic 117:335–340

    Article  CAS  Google Scholar 

  • Kanchanapoom K, Promsom N (2012) Micropropagation and in vitro germplasm conservation of endangered Musa balbisiana ‘Kluai Hin’ (BBB group). Afr J Biotechnol 11(24):6464–6469

    CAS  Google Scholar 

  • Khatri A, Dahot MU, Khan IA, Nizamani GS (2010) An efficient method of protoplast isolation in banana (Musa spp.). Pak J Bot 42(2):1267–1271

    Google Scholar 

  • Klercker JAF (1892) Eine methode zur isolierung lebender protoplasten. Ofvers Vetensk-Akad Ffrh Stokh 9:463–475

    Google Scholar 

  • Kosky RG, Silva MF, Perez LP, Gilliard T, Martinez FB, Vega MR, Milian MC, Mendoza EQ (2002) Somatic embryogenesis of the banana hybrid cultivar FHIA-18 (AAAB) in liquid medium and scaled-up in a bioreactor. Plant Cell Tiss Organ Cult 68:21–26

    Article  CAS  Google Scholar 

  • Kovács G, Sági L, Jacon G, Arinaitwe G, Busogoro JP, Thiry E, Strosse H, Swennen R, Remy S (2013) Expression of a rice chitinase gene in transgenic banana (‘Gros Michel’, AAA genome group) confers resistance to black leaf streak disease. Transgenic Res 22:117–130

    Article  PubMed  CAS  Google Scholar 

  • Krasikov V, Dekker HL, Rep M, Takken FL (2011) The tomato xylem sap protein XSP10 is required for full susceptibility to Fusarium wilt disease. J Exp Bot 62:963–973

    Article  CAS  PubMed  Google Scholar 

  • Kulkarni VM, Bapat VA (2013) Somatic embryogenesis and plant regeneration from cell suspension cultures of Rajeli (AAB), an endangered banana cultivar. J Plant Biochem Biotechnol 22(1):132–137

    Article  CAS  Google Scholar 

  • Kulkarni VM, Suprasanna P, Bapat VA (2006) Plant regeneration through multiple shoot formation and somatic embryogenesis in a commercially important and endangered Indian banana cv. Rajeli. Curr Sci 90:842–846

    Google Scholar 

  • Kulkarni VM, Ganapathi TR, Suprasanna P, Bapat VA (2007) In vitro mutagenesis in banana (Musa spp.) using gamma irradiation. In: Jain SM, Häggman H (eds) Protocols for micropropagation of woody trees and fruits. Springer, Dordrecht, pp 543–559

    Chapter  Google Scholar 

  • Lee SC, Hwang IS, Choi HW, Hwang BK (2008) Involvement of the pepper antimicrobial protein CaAMP1 gene in broad spectrum disease resistance. Plant Physiol 148:1004–1020

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Lee EJ, Moh SH, Paek KY (2011) Influence of inoculum density and aeration volume on biomass and bioactive compound production in bulb-type bubble bioreactor cultures of Eleutherococcus koreanum Nakai. Bioresour Technol 102:7165–7170

    Article  CAS  PubMed  Google Scholar 

  • Lorenc-Kukuła K, Zuk M, Kulma A, Czemplik M, Kostyn K, Skala J, Starzycki M, Szopa J (2009) Engineering flax with the GT family 1 Solanum sogarandinum glycosyltransferase SsGT1 confers increased resistance to Fusarium infection. J Agric Food Chem 57:6698–6705

    Article  PubMed  CAS  Google Scholar 

  • Mahadev SR, Kathithachalam A, Marimuthu M (2011) An efficient protocol for large-scale plantlet production from male floral meristems of Musa spp. cultivars Virupakshi and Sirumalai. In Vitro Cell Develop Biol Plant 47:611–617

    Article  CAS  Google Scholar 

  • Mahdavi F, Sariah M, Maziah M (2012) Expression of rice thaumatin-like protein gene in transgenic banana plants enhances resistance to Fusarium wilt. Appl Biochem Biotechnol 166:1008–1019

    Article  CAS  PubMed  Google Scholar 

  • Mahdi R, Islam MJ, Rahman MA, Biswas A, Azam FMS, Rahmatullah M (2014) In vitro regeneration protocol for anupam and chini champa: Two banana (Musa sapientum) cultivars of Bangladesh. Am-Eurasian J Sustain Agric 8(1):28–33

    Google Scholar 

  • Makara AM, Rubaihayo PR, Magambo MJS (2010) Carry-over effect of thidiazuron on banana in vitro proliferation at different culture cycles and light incubation conditions. Afr J Biotechnol 9:3079–3085

    CAS  Google Scholar 

  • Marín DH, Romero RA, Guzman M, Sutton TB (2003) Black Sigatoka: an increasing threat to banana cultivation. Plant Dis 87:208–222

    Article  Google Scholar 

  • Matsumoto K, Oka S (1998) Plant regeneration from protoplasts of a brazilian dessert banana (Musa spp., AAB GROUP). Acta Hortic 490:455–462

    Article  Google Scholar 

  • Matsumoto K, Crepy L, Teixeira JB, Ferreira FR (1988) Isolation, culture and fusion of banana bract protoplast. In: International Rice Research Institute (IRRI) and Academia Sinica (eds) Genetic manipulation in crops. Cassell Tycooly, Philadelphia, pp 414–415

    Google Scholar 

  • Megia R, Haicour R, Rossignol L, Sihachakr D (1992) Callus formation from cultured protoplasts of banana (Musa spp.). Plant Sci 85:91–98

    Article  Google Scholar 

  • Megia R, Hacour R, Tizroutine S, Bui Trang V, Rossignol L, Sihachakr D, Schwendiman J (1993) Plant regeneration from cultured protoplasts of the cooking banana cv. Bluggoe (Musa spp., ABB group). Plant Cell Rep 13:41–44

    Article  CAS  PubMed  Google Scholar 

  • Mohandas S, Sowmyaa HD, Saxenaa AK, Meenakshia S, Rania RT, Mahmood R (2013) Transgenic banana cv. Rasthali (AAB, Silk gp) harboring Ace-AMP1 gene imparts enhanced resistance to Fusarium oxysporum f.sp. cubense race 1. Sci Hortic 164:392–399

    Article  CAS  Google Scholar 

  • Msogoya TJ, Grout BW, Maerere AP (2011) Performance of micropropagation-induced off-type of East African highland banana (Musa AAA-East Africa). J Anim Plant Sci 10:1334–1338

    Google Scholar 

  • Namanya P, Mutumba G, Magambo SM, Tushemereirwe W (2014) Developing a cell suspension system for Musa-AAA-EAcv. ‘Nakyetengu’: a critical step for genetic improvement of Matooke East African Highland bananas. In Vitro Cell Dev Biol Plant 50:442–450

    Article  Google Scholar 

  • Nasution RE (1991) A taxonomic study of the species Musa acuminata Colla with its intraspecific taxa in Indonesia. Memoirs of Tokyo Univ Agric 23:1–122

    Google Scholar 

  • Ngomuo M, Mneney E, Ndakidemi P (2013) The effects of auxins and cytokinin on growth and development of (Musa sp.) var. “Yangambi” explants in tissue culture. Am J Plant Sci 4(11):2174–2180

    Article  CAS  Google Scholar 

  • Ngomuo M, Mneney E, Ndakidemi PA (2014a) The in vitro propagation techniques for producing banana using shoot tip cultures. Am J Plant Sci 5:1614–1622

    Article  CAS  Google Scholar 

  • Ngomuo M, Mneney E, Ndakidemi P (2014b) Control of lethal browning by using ascorbic acid on shoot tip cultures of a local Musa spp. (Banana) cv. Mzuzu in Tanzania. Afr J Biotechnol 13(16):1721–1725

    Article  CAS  Google Scholar 

  • Novak FJ, Afza R, Van Duren M, Perea-Dallos M, Conger BV, Xiaolang T (1989) Somatic embryogenesis and plant regeneration in suspension cultures of dessert (AA and AAA) and cooking (ABB) bananas (Musa spp.). Nature Biotechnol 7:154–159

    Article  Google Scholar 

  • Ortiz R, Ferris RSB, Vuylsteke DR (1995) Banana and plantain breeding. In: Gowen S (ed) Bananas and plantains. Chapman & Hall, London, pp 110–146

    Chapter  Google Scholar 

  • Osuji JO, Okoli BE, Vuylsteke D, Ortiz R (1997) Multivariate pattern of quantitative trait variation in triploid banana and plantain cultivars. Sci Hortic 71:197–202

    Article  Google Scholar 

  • Panis B, Wauwe AW, Swennen R (1993) Plant regeneration through direct somatic embryogenesis from protoplasts of banana (Musa spp.). Plant Cell Rep 12:403–407

    Article  CAS  PubMed  Google Scholar 

  • Perez BC, Reyes M, Rojas L, Ocana B, Perez B, Kosky RG, Angenon G (2012) Establishment of embryogenic cell suspension cultures and Agrobacterium-mediated transformation in banana cv. ‘Dwarf Cavendish’ (Musa AAA): effect of spermidine on transformation efficiency. Plant Cell Tiss Organ Cult 111:79–90

    Article  CAS  Google Scholar 

  • Pervin R, Saiful Azam FM, Morshed MT, Rahman S, Hero MKA, Asaduzzaman RM (2013) Natural growth substances has effective role in callus culture of banana (Musa spp.) cultivar ‘Anupam’ (AAB Genome, Sapientum Subgroup). Am-Eurasian J Sustain Agric 7(3):149–154

    Google Scholar 

  • Pillay M, Tripathi L (2007) Banana. In: Kole C (ed) Fruits and nuts, vol 4, Genome mapping and molecular breeding in plants. Springer, Berlin, pp 281–301

    Chapter  Google Scholar 

  • Ploetz RC (2015) Management of Fusarium wilt of banana: a review with special reference to tropical race 4. Crop Prot 73:7–15

    Article  CAS  Google Scholar 

  • Ploetz RC, Evans EA (2015) The future of global banana production. In: Janick J (ed) Horticultural reviews, vol 43, 1st edn. Wiley-Blackwell, Wiley, Hoboken, pp 311–352

    Google Scholar 

  • Ploetz RC, Kema GHJ, Ma LJ (2015) Impact of diseases on export and smallholder production of banana. Annu Rev Phytopathol 53:269–288

    Article  CAS  PubMed  Google Scholar 

  • Rahman S, Biswas N, Hassan MM, Ahmed MG, Mamun ANK, Islam MR, Moniruzzaman M, Haque ME (2013) Micropropagation of banana (Musa sp.) cv. Agnishwar by in vitro shoot tip culture. Int Res J Biotechnol 4(4):83–88

    Google Scholar 

  • Rashid K, Nezhadahmadi A, Othman RY, Ismail NA, Azhar S, Efzueni S (2012) Micropropagation of ornamental plant Musa beccarii through tissue culture technique using suckers and male buds as explants. Life Sci J 9(4):2046–2053

    Google Scholar 

  • Remakanthan A, Menon TG, Soniya EV (2014) Somatic embryogenesis in banana (Musa acuminata AAA cv. Grand Naine): effect of explant and culture conditions. In Vitro Cell Dev Biol–Plant 50:127–136

    Article  CAS  Google Scholar 

  • Rezazadeh R, Niedz RP (2015) Protoplast isolation and plant regeneration of guava (Psidium guajava L.) using experiments in mixture-amount design. Plant Cell Tiss Organ Cult 122:585–604

    Article  CAS  Google Scholar 

  • Rezazadeh R, Williams R, Harrison D (2011) Factors affecting mango (Mangifer indica L.) protoplast isolation and culture. Sci Hortic 130:214–221

    Article  Google Scholar 

  • Rhodes PL (1964) A new banana disease in Fiji. Commonw Phytopathol News 10:38–41

    Google Scholar 

  • Robinson JC, Saúco VG (2010) Bananas and plantains. In: Crop production science in horticulture series 19, 2nd edn. Centre for Agricultural Bioscience International, Wallingford, UK

    Google Scholar 

  • Rout GR, Mohapatra A, Jain SM (2006) Tissue culture of ornamental pot plant: a critical review on present scenario and future prospects. Biotechnol Adv 24:531–560

    Article  CAS  PubMed  Google Scholar 

  • Sadik K, Rubaihayo PR, Magambo MJS, Pillay M (2007) Generation of cell suspensions of East African highland bananas through scalps. Afr J Biotechnol 6:1352–1357

    CAS  Google Scholar 

  • Shankar CS, Balaji P, Sekar DS (2014) Mass propagation of banana (Musa sp.) cv. Grand Naine through direct organogenesis by benzyl adenine purine and kinetin. J Acad Indus Res 3(2):92–97

    Google Scholar 

  • Sholi NJY, Chaurasia A, Agrawal A, Sarin NB (2009) ABA enhances plant regeneration of somatic embryos derived from cell suspension cultures of plantain cv. Spambia (Musa sp.). Plant Cell Tiss Organ Cult 99:133–140

    Article  CAS  Google Scholar 

  • Simmonds NW, Shepherd K (1955) The taxonomy and origins of the cultivated bananas. J Linn Soc (Bot) London 55:302–312

    Article  Google Scholar 

  • Sipen P, Davey MR (2012) Effects of N6-benzylaminopurine and indole acetic acid on in vitro shoot multiplication, nodule-like meristem proliferation and plant regeneration of Malaysian bananas (Musa spp.). Trop Life Sci Res 23(2):67–80

    CAS  PubMed  PubMed Central  Google Scholar 

  • Strosse H, Schoofs H, Panis B, Andre E, Reyniers K, Swennen R (2006) Development of embryogenic cell suspensions from shoot meristematic tissue in bananas and plantains (Musa spp.). Plant Sci 170:104–112

    Article  CAS  Google Scholar 

  • Sultan MT, Khan MH, Hakim ML, Mamun ANK, Morshed MA, Islam MR, Islam MR (2011) In vitro plant regeneration from male flowers of banana. Int J Biosci 1:1–11

    CAS  Google Scholar 

  • Tan D, Fu L, Han B, Sun X, Zheng P, Zhang J (2015) Identification of an endophytic antifungal bacterial strain isolated from the rubber tree and its application in the biological control of banana Fusarium wilt. PLoS One 10(7):e0131974

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Torres JL, Kosky RJ, Perez NM, Alvarez DR, Cabrera AR, Jova MC, Pino AS, Vega VM, Perez MB, Roux N (2012) New explant for somatic embryogenesis induction and plant regeneration from diploid banana (‘Calcutta 4’, Musa AA). Biotecnol Veg 12(1):25–31

    Google Scholar 

  • Tripathi L, Tripathi JN, Tushemereirwe WK (2008a) Rapid and efficient production of transgenic East African highland banana (Musa spp.) using intercalary meristematic tissues. Afr J Biotechnol 7(10):1438–1445

    Google Scholar 

  • Tripathi L, Odipio J, Tripathi JN, Tusiime G (2008b) A rapid technique for screening banana cultivars for resistance to Xanthomonas wilt. Eur J Plant Pathol 121:9–19

    Article  Google Scholar 

  • Tripathi JN, Muwonge A, Tripathi L (2012) Efficient regeneration and transformation of plantain cv. “Gonja manjaya” (Musa spp. AAB) using embryogenic cell suspensions. In Vitro Cell Dev Biol Plant 48:216–224

    Article  Google Scholar 

  • Tripathi L, Babirye A, Roderick H, Tripathi JN, Changa C, Urwin PE, Tushemereirwe WK, Coyne D, Atkinson HJ (2015) Field resistance of transgenic plantain to nematodes has potential for future African food security. Sci Rep 5:8127

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Tushemereirwe W, Kangire A, Ssekiwoko F, Offord LC, Crozier J, Boa E et al (2004) First report of Xanthomonas campestris pv. musacearum on banana in Uganda. Plant Pathol 53:802

    Article  Google Scholar 

  • Uma S, Lakshmi, Saraswathi MS, Akbar A, Mustaffa MM (2011) Embryo rescue and plant regeneration in banana (Musa spp.). Plant Cell Tiss Organ Cult 105:105–111

    Article  CAS  Google Scholar 

  • Uma S, Lakshmi S, Saraswathi MS, Akbar A, Mustaffa MM (2012) Plant regeneration through somatic embryogenesis from immature and mature zygotic embryos of Musa acuminata spp. Burmannica. In Vitro Cell Dev Biol Plant 48(5):539–545

    Article  Google Scholar 

  • Vishnevetsky J, White TL Jr, Palmateer AJ, Flaishman M, Cohen Y, Elad Y, Velcheva M, Hanania U, Sahar N, Dqani O, Perl A (2011) Improved tolerance toward fungal diseases in transgenic Cavendish banana (Musa spp. AAA group) cv. Grand Nain. Grand Nain Transgenic Res 20(1):61–72

    Article  CAS  PubMed  Google Scholar 

  • Vuylsteke D, Ortiz R, Pasberg-Gauhl C, Gauhl F, Gold C, Ferris S, Speijer P (1993) Plantain and banana research at the International Institute of Tropical Agriculture. Hortic Sci 28(9):874–971

    Google Scholar 

  • Wilken D, Gonzalez EJ, Gerth A, Kosky RG, Schumann A, Claus D (2014) Effect of immersion systems, lighting, and TIS designs on biomass increase in micropropagating banana (Musa spp. cv. ‘Grande Naine’ AAA). In Vitro Cell Dev Biol Plant 50:582–589

    Article  CAS  Google Scholar 

  • Xiao W, Huang XL, Huang X, Chen YP, Dai XM, Zhao JT (2007) Plant regeneration from protoplasts of Musa acuminata cv. Mas (AA) via somatic embryogenesis. Plant Cell Tiss Organ Cult 90:191–200

    Article  Google Scholar 

  • Yu C, Yang X, Wang Z, Qi J, Wu Y (2013) Research on preparation method of protoplasts from callus of grape. J Fruits Sci 2013-03

    Google Scholar 

  • Zhou X, Wei Y, Zhu H, Wang Z, Lin J, Lu L, Tang K (2008) Protoplast formation, regeneration and transformation from the taxol-producing fungus Ozonium sp. Afr J Biotechnol 7:2017–2024

    Article  CAS  Google Scholar 

Download references

Acknowledgments

We sincerely apologize to the authors whose valuable studies could not be cited due to limited space. We acknowledge the funding from UMRG (RP005A-13BIO) and RU005D-2014 University of Malaya.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Norzulaani Khalid .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2016 Springer Science+Business Media Singapore

About this chapter

Cite this chapter

Khalid, N., Tan, B.C. (2016). A to Z on Banana Micropropagation and Field Practices. In: Anis, M., Ahmad, N. (eds) Plant Tissue Culture: Propagation, Conservation and Crop Improvement. Springer, Singapore. https://doi.org/10.1007/978-981-10-1917-3_6

Download citation

Publish with us

Policies and ethics