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In Vitro Propagation and Assessment of the Genetic Fidelity of Musa acuminata (AAA) cv. Vaibalhla Derived from Immature Male Flowers

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Abstract

An efficient in vitro propagation method has been developed for the first time for Musa acuminata (AAA) cv. Vaibalhla, an economically important banana cultivar of Mizoram, India. Immature male flowers were used as explants. Murashige and Skoog’s (MS) medium supplemented with plant growth regulators (PGRs) were used for the regeneration process. Out of different PGR combinations, MS medium supplemented with 2 mg L−1 6-benzylaminopurine (BAP) + 0.5 mg L−1 α-naphthalene acetic acid (NAA) was optimal for production of white bud-like structures (WBLS). On this medium, explants produced the highest number of buds per explant (4.30). The highest percentage (77.77) and number (3.51) of shoot formation from each explants was observed in MS medium supplemented with 2 mg L−1 kinetin + 0.5 mg L−1 NAA. While MS medium supplemented with a combination of 2 mg L−1 BAP + 0.5 mg L−1 NAA showed the maximum shoot length (14.44 cm). Rooting efficiency of the shoots was highest in the MS basal medium without any PGRs. The plantlets were hardened successfully in the greenhouse with 96 % survival rate. Random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers were employed to assess the genetic stability of in vitro regenerated plantlets of M. acuminata (AAA) cv. Vaibalhla. Eight RAPD and 8 ISSR primers were successfully used for the analysis from the 40 RAPD and 30 ISSR primers screened initially. The amplified products were monomorphic across all the regenerated plants and were similar to the mother plant. The present standardised protocol will find application in mass production, conservation and genetic transformation studies of this commercially important banana.

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Acknowledgments

The authors gratefully acknowledge the University Grants Commission (UGC), New Delhi for providing the Meritorious Fellowship to Lalremsiami Hrahsel and Department of Biotechnology (DBT) and Govt. of India for providing the Bioinformatics Infrastructure Facility (BIF) which was used for the data analysis.

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Authors and Affiliations

  1. Department of Biotechnology, School of Life Sciences, Mizoram University, Aizawl, 796004, India

    Lalremsiami Hrahsel & Robert Thangjam

  2. Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, 781039, India

    Adreeja Basu & Lingaraj Sahoo

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  1. Lalremsiami Hrahsel
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  2. Adreeja Basu
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  3. Lingaraj Sahoo
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  4. Robert Thangjam
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Correspondence to Robert Thangjam.

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Hrahsel, L., Basu, A., Sahoo, L. et al. In Vitro Propagation and Assessment of the Genetic Fidelity of Musa acuminata (AAA) cv. Vaibalhla Derived from Immature Male Flowers. Appl Biochem Biotechnol 172, 1530–1539 (2014). https://doi.org/10.1007/s12010-013-0637-9

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  • DOI: https://doi.org/10.1007/s12010-013-0637-9

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