Abstract
Flow cytometry is a technique which permits the characterisation of individual cells in populations, in terms of distributions in their properties such as DNA content, protein content, viability, enzyme activities and so on. We review the technique, and some of its recent applications to microbiological problems. It is concluded that cellular heterogeneity, in both batch and continuous axenic cultures, is far greater than is normally assumed. This has important implications for the quantitative analysis of microbial processes.
‘In contrast to standard microbiological, genetic or biochemical techniques, this method provides information on individual cells, and not just average values for the popUlation. This ability to analyze individual cells is invaluable in studying the distribution of cell parameters in a polydisperse population. and gives access to information that cannot be obtained in any other way.’
Boye & Løbner-Olesen 1990
‘Flow cytometry has revolutionized the study of the cell cycle of eukaryotes. It is also possible to apply the flow cytometry principles to bacteria....The importance of the flow cytometry results should not be underestimated. They provide a crucial link in the analysis of the division cycle....While other experiments have substantially supported the initial membrane-elution results. the flow eytometry results determine the pattern of DNA replication without any perturbations of the cell.’
Cooper 1991
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Kell, D.B., Ryder, H.M., Kaprelyants, A.S., Westerhoff, H.V. (1992). Quantifying heterogeneity: flow cytometry of bacterial cultures. In: Stouthamer, A.H. (eds) Quantitative Aspects of Growth and Metabolism of Microorganisms. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-2446-1_3
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